O67216 (DAPA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	aq_1143

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 294

294

Dihydrodipicolinate synthase HAMAP MF_00418

PRO_0000103081

Regions

Region

47 – 48

2

Pyruvate binding By similarity

Sites

Active site

161

1

Schiff-base intermediate with substrate By similarity

Binding site

105

1

Pyruvate By similarity

Site

132

1

Involved in proton transfer during cleavage By similarity

Secondary structure

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294

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

20 – 31

12

Turn

32 – 34

3

Beta strand

37 – 42

6

Turn

43 – 46

4

Helix

47 – 49

3

Helix

52 – 66

15

Beta strand

69 – 75

7

Helix

81 – 93

13

Beta strand

97 – 102

6

Helix

111 – 124

14

Beta strand

129 – 133

5

Helix

135 – 138

4

Helix

144 – 153

10

Beta strand

157 – 162

6

Helix

167 – 177

11

Beta strand

181 – 187

7

Helix

188 – 190

3

Helix

191 – 196

6

Beta strand

201 – 205

5

Helix

206 – 208

3

Helix

211 – 223

13

Helix

226 – 242

17

Beta strand

245 – 247

3

Helix

250 – 258

9

Helix

275 – 287

13

Sequences

	Sequence		Length	Mass (Da)
	O67216 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 0778B824E6619E31	FASTA	294	32,670
	10 20 30 40 50 60 MFQGSIVALI TPFKEGEVDY EALGNLIEFH VDNGTDAILV CGTTGESPTL TFEEHEKVIE 70 80 90 100 110 120 FAVKRAAGRI KVIAGTGGNA THEAVHLTAH AKEVGADGAL VVVPYYNKPT QRGLYEHFKT 130 140 150 160 170 180 VAQEVDIPII IYNIPSRTCV EISVDTMFKL ASECENIVAS KESTPNMDRI SEIVKRLGES 190 200 210 220 230 240 FSVLSGDDSL TLPMMALGAK GVISVANNVM PREVKELIRA ALEGDFRRAR EIHYYLHDLF 250 260 270 280 290 KVLFIETNPI PVKTACWMLG MCEKEFRLPL TEMSPENENK LREVLKKYNL PLKN

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC07169.1.

PIR

E70398.

RefSeq

NP_213780.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EHH	X-ray	1.90	A/C/D/E	1-294	[»]

ProteinModelPortal

O67216.

SMR

O67216. Positions 1-294.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1192562.

GenomeReviews

Gene locus aq_1143 in contig AE000657_GR.

KEGG

aae:aq_1143.

NMPDR

fig|224324.1.peg.795.

PATRIC

20959542. VBIAquAeo85532_0891.

Phylogenomic databases

HOGENOM

HBG358848.

OMA

FMLCGGH.

PhylomeDB

O67216.

ProtClustDB

PRK03170.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_1143-MONOMER.

Family and domain databases

HAMAP

MF_00418. DapA.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K01714.

PANTHER

PTHR12128. DHDPS. 1 hit.

Pfam

PF00701. DHDPS. 1 hit.
[Graphical view]

PIRSF

PIRSF001365. DHDPS. 1 hit.

PRINTS

PR00146. DHPICSNTHASE.

TIGRFAMs

TIGR00674. DapA. 1 hit.

PROSITE

PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DAPA_AQUAE

Accession

Primary (citable) accession number: O67216

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program