O67213 (ASSY_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate synthase
EC=6.3.4.5

Alternative name(s):
Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	aq_1140

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm By similarity HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW argininosuccinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148562
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity
Sites
	Binding site	35	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	86	1	Citrulline By similarity
	Binding site	116	1	ATP; via amide nitrogen By similarity
	Binding site	118	1	Aspartate By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	122	1	Citrulline By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	175	1	Citrulline By similarity
	Binding site	184	1	Citrulline By similarity
	Binding site	261	1	Citrulline By similarity
	Binding site	273	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67213 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2BCBA9D027CE33CF	FASTA	401	45,848
	10 20 30 40 50 60 MKKKRVILAY SGGLDTSIIV RWLTEKGYEV ITYTADVGQG EELSEIPEKA RRAGAIEAIV 70 80 90 100 110 120 EDLKETFAEN YCLPTLRALA LYEGKYPLTA ALSRPLIAER LVYYAEKFNA DYVAHGSTGK 130 140 150 160 170 180 GNDQVRFELS VWALNPDIEV LAPVREWEFK SREEQVEYAQ RFNIPVKATK EKPYSIDRNL 190 200 210 220 230 240 WGVSIECGPL EDPWQEPPQD AYQITQSPEE APDEPEYVTV GFEKGKPVYL NGERYEEQWK 250 260 270 280 290 300 LIANLNEIAG RHGVGRIDMV ENRLVGIKSR EIYEAPGAMV LYEAYRDLLS LVLDRFTFHY 310 320 330 340 350 360 FLTHIPHEYA KLVYEGLWFT PLREALDAFT NKIAEFATGE VRLKLYKGSV SVVGRRSPNS 370 380 390 400 LYVEELATYS EKDQFDQIAG KHFTKVWGLP LKVLGRVRKG K

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07170.1.
PIR	B70398.
RefSeq	NP_213777.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67213.
SMR	O67213. Positions 5-399.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192559.
GenomeReviews	Gene locus aq_1140 in contig AE000657_GR.
KEGG	aae:aq_1140.
NMPDR	fig|224324.1.peg.792.
PATRIC	20959536. VBIAquAeo85532_0888.
Phylogenomic databases
HOGENOM	HBG335267.
OMA	DHIEDRV.
PhylomeDB	O67213.
ProtClustDB	PRK00509.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1140-MONOMER.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR023434. Arginosuc_synth_type_1_subfam. IPR024074. AS_cat/multimer_dom_body. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.90.1260.10. G3DSA:3.90.1260.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01940.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. ArgG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_AQUAE

Accession

Primary (citable) accession number: O67213

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program