Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O67213 (ASSY_AQUAE)

Last modified February 9, 2010. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase
Gene names
Name: argG
Ordered Locus Names: aq_1140
OrganismAquifex aeolicus [Complete proteome] [HAMAP]
Taxonomic identifier63363 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Hide | Top

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005

Subunit structure

Homotetramer By similarity. HAMAP MF_00005

Subcellular location

Cytoplasm Probable HAMAP MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Hide | Top

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Argininosuccinate synthase HAMAP MF_00005
PRO_0000148562

Regions

Nucleotide binding9 – 179ATP By similarity

Sites

Binding site351ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site861Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1751Citrulline By similarity
Binding site1841Citrulline By similarity
Binding site2611Citrulline By similarity
Binding site2731Citrulline By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
O67213-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 2BCBA9D027CE33CF

FASTA40145,848
        10         20         30         40         50         60 
MKKKRVILAY SGGLDTSIIV RWLTEKGYEV ITYTADVGQG EELSEIPEKA RRAGAIEAIV 

        70         80         90        100        110        120 
EDLKETFAEN YCLPTLRALA LYEGKYPLTA ALSRPLIAER LVYYAEKFNA DYVAHGSTGK 

       130        140        150        160        170        180 
GNDQVRFELS VWALNPDIEV LAPVREWEFK SREEQVEYAQ RFNIPVKATK EKPYSIDRNL 

       190        200        210        220        230        240 
WGVSIECGPL EDPWQEPPQD AYQITQSPEE APDEPEYVTV GFEKGKPVYL NGERYEEQWK 

       250        260        270        280        290        300 
LIANLNEIAG RHGVGRIDMV ENRLVGIKSR EIYEAPGAMV LYEAYRDLLS LVLDRFTFHY 

       310        320        330        340        350        360 
FLTHIPHEYA KLVYEGLWFT PLREALDAFT NKIAEFATGE VRLKLYKGSV SVVGRRSPNS 

       370        380        390        400 
LYVEELATYS EKDQFDQIAG KHFTKVWGLP LKVLGRVRKG K

« Hide

Hide | Top

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC07170.1.
PIRB70398.
RefSeqNP_213777.1.

3D structure databases

SMRO67213. Positions 5-399.
ModBaseSearch...

Genome annotation databases

GeneID1192559.
GenomeReviewsGene locus aq_1140 in contig AE000657_GR.
KEGGaae:aq_1140.
NMPDRfig|224324.1.peg.792.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG335267.
OMAKRVILAY.
PhylomeDBO67213.

Enzyme and pathway databases

BioCycAAEO224324:AQ_1140-MONOMER.
BRENDA6.3.4.5. 189781.

Family and domain databases

HAMAPMF_00005. Arg_succ_synth_type1.
[Tree]
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR11587. Arginosuc_synth. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameASSY_AQUAE
AccessionPrimary (citable) accession number: O67213
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: February 9, 2010
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents