Reviewed, UniProtKB/Swiss-Prot O67209 (PROB_AQUAE)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	aq_1134

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109630
Regions
	Domain	265 – 342	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	O67209-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 8AA1893E8B72CC0F	FASTA	356	39,054
	10 20 30 40 50 60 MRIVFKIGSN LLETDEGDID LSFLSKLAEG IKKLHSFGDK VLIVSSGAVL SGAKKLGIKE 70 80 90 100 110 120 KPKDLTLKQA LASVGQSYLM HLYDTIFSNY GLKVGQVLLT KDVFSKEKEE RFKNAKSTLE 130 140 150 160 170 180 KLLELGVVPV INENDAVAVE ELVFGDNDFL AVYVSFMVNA DLLVIFSSAG GLKDDKDRII 190 200 210 220 230 240 PVVEDIEKVF KYVRGTGTSF GTGGMRSKLE ATRLATTLNI PVIITSKEEN ILDLRNLKTK 250 260 270 280 290 300 GTLFKPSKRK LRNALKVIAT LEEPKGIIVV DRGAQEALKA GKSLLPAGVV SVQGNFKRGD 310 320 330 340 350 VVSIQNEEGL IIGKGKVNFS SEEIEKIKGL KTSEVRKLLN TTKDEVIHRD NMVIFL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07171.1.
PIR	F70397.
RefSeq	NP_213773.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1192555.
GenomeReviews	Gene locus aq_1134 in contig AE000657_GR.
KEGG	aae:aq_1134.
NMPDR	fig|224324.1.peg.788.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67209.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1134-MON.
BRENDA	2.7.2.11. 189781.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR004521. Unchar_dom_2. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit. TIGR00451. unchar_dom_2. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_AQUAE

Accession

Primary (citable) accession number: O67209

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)