Reviewed, UniProtKB/Swiss-Prot O67166 (PROA_AQUAE)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	aq_1071

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Gamma-glutamyl phosphate reductase		PRO_0000189684

Sequences

	Sequence		Length	Mass (Da)
	O67166-1 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: BE6500C58A2B2D61	FASTA	435	49,119
	10 20 30 40 50 60 MNYIEEKVNR ARSVLRELTS LKTSVKNETL LKVAELIDKN RDYIKEENRK DVEKAKEMGL 70 80 90 100 110 120 RPAVVDRLVL NDKRIDGMVK VLKDVASLPD PVGEIIKMWN LPNGLKVGRM RVPLGVIFIV 130 140 150 160 170 180 YESRPNVTIE ASSLCMKSSN AVILRGGKEA INSNKALVNL IKEACRETGF PEDAVQFIDR 190 200 210 220 230 240 SEREIVWEIL KMEGKIDVAI PRGGESLIRA VVEHAKVPVI KHYKGVCNIY VDDEADLEKA 250 260 270 280 290 300 YHIVYNAKVQ RPSVCNAVEN LVINRKILKE FFPKMAYYLG KAGVELRCDE ESLQVIKENP 310 320 330 340 350 360 KLSFVNAKPA TEEDYYEEFL DLILAVKVVD DVDEAIAFIE KYGSKHSDAI ITENYTKAMK 370 380 390 400 410 420 FLREVDSAAV YVNASTRFTD GNEFGLGAEM GISTDKIHAR GPMALEELTI PKFVILGEGQ 430 VRDNFGVPEE WMKEF

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07119.1. Different initiation.
PIR	C70392.
RefSeq	NP_213729.1.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GeneID	1193800.
GenomeReviews	Gene locus aq_1071 in contig AE000657_GR.
KEGG	aae:aq_1071.
NMPDR	fig|224324.1.peg.744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67166.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1071-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_AQUAE

Accession

Primary (citable) accession number: O67166

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 20, 2001
Last modified:	November 25, 2008
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)