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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29ZincBy similarity1
Metal bindingi209ZincBy similarity1
Metal bindingi234ZincBy similarity1
Metal bindingi238ZincBy similarity1
Binding sitei269ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:aq_1068
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001593401 – 495Cysteine--tRNA ligaseAdd BLAST495

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_1068.

Structurei

3D structure databases

ProteinModelPortaliO67163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi31 – 41"HIGH" regionAdd BLAST11
Motifi266 – 270"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245250.
InParanoidiO67163.
KOiK01883.
OMAiYQIDIHA.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

O67163-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRIYNTLS GKVEEFVPIN PPKVLIYTCG VTVYDDSHVG HGRSLIVFDV
60 70 80 90 100
FRRFLEHLGY QVKFVRNFTD VDDKIINRAK QECTDFMTIA DRYIARYYVD
110 120 130 140 150
MENIRVRPAD VEPRVTEHIP EIIEVIQKLV EKGYAYVVEG DVYFSVKKFK
160 170 180 190 200
DYGKLSKRDI EELIAGARVE PSEKKRDPLD FALWKASKAG EPAWDSPWGK
210 220 230 240 250
GRPGWHTECV AMVFKHLGET IDIHGGGLDL VFPHHENEIA QAEAITGKPF
260 270 280 290 300
ARYWMHNGLV TVGGQKMSKS LGNYVTLREV YTKYHPDVLR LLVLFTHYRS
310 320 330 340 350
PLDFSWEKME ETLKAYERLK NAIEDLELLK KLQVVESKEG GTHPLYEQVK
360 370 380 390 400
EFEENFYASL SDDFNTPEAL SHVYKLVGEL NKVKNKAYSE GKITDRELSA
410 420 430 440 450
YEFASKSLLN TMKKIFGLLE DLYPECKVER VVERELEAGQ VFDEKLIQIL
460 470 480 490
IEARNIARKE KVFKVADYIR DKLKELGIVL EDTPAGTKWK KREGA
Length:495
Mass (Da):57,136
Last modified:August 1, 1998 - v1
Checksum:iA454658B2BBAE8A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07125.1.
PIRiH70391.
RefSeqiNP_213726.1. NC_000918.1.
WP_010880664.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07125; AAC07125; aq_1068.
GeneIDi1193797.
KEGGiaae:aq_1068.
PATRICi20959420. VBIAquAeo85532_0830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07125.1.
PIRiH70391.
RefSeqiNP_213726.1. NC_000918.1.
WP_010880664.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07125; AAC07125; aq_1068.
GeneIDi1193797.
KEGGiaae:aq_1068.
PATRICi20959420. VBIAquAeo85532_0830.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245250.
InParanoidiO67163.
KOiK01883.
OMAiYQIDIHA.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_AQUAE
AccessioniPrimary (citable) accession number: O67163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.