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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.By similarity

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NAD1 Publication1
Binding sitei78NAD; via carbonyl oxygen1 Publication1
Binding sitei120NAD1 Publication1
Active sitei152NucleophileBy similarity1
Sitei179Activates thiol group during catalysisBy similarity1
Binding sitei182Glyceraldehyde 3-phosphateBy similarity1
Binding sitei183NADBy similarity1
Binding sitei197Glyceraldehyde 3-phosphateBy similarity1
Binding sitei233Glyceraldehyde 3-phosphateBy similarity1
Binding sitei322NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NAD1 Publication2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseBy similarity (EC:1.2.1.12By similarity)
Short name:
GAPDHBy similarity
Alternative name(s):
NAD-dependent glyceraldehyde-3-phosphate dehydrogenaseBy similarity
Gene namesi
Name:gap
Ordered Locus Names:aq_1065
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001456291 – 342Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST342

Proteomic databases

PRIDEiO67161.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224324.aq_1065.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi12 – 21Combined sources10
Beta strandi28 – 33Combined sources6
Helixi38 – 46Combined sources9
Turni49 – 51Combined sources3
Beta strandi58 – 60Combined sources3
Beta strandi62 – 67Combined sources6
Beta strandi70 – 75Combined sources6
Helixi80 – 82Combined sources3
Helixi86 – 88Combined sources3
Beta strandi91 – 95Combined sources5
Beta strandi97 – 99Combined sources3
Helixi103 – 106Combined sources4
Turni107 – 109Combined sources3
Helixi110 – 112Combined sources3
Beta strandi115 – 121Combined sources7
Beta strandi127 – 129Combined sources3
Turni132 – 134Combined sources3
Helixi136 – 138Combined sources3
Turni141 – 143Combined sources3
Beta strandi146 – 148Combined sources3
Helixi152 – 168Combined sources17
Beta strandi170 – 180Combined sources11
Beta strandi185 – 189Combined sources5
Turni195 – 198Combined sources4
Helixi201 – 203Combined sources3
Beta strandi206 – 208Combined sources3
Helixi214 – 218Combined sources5
Helixi221 – 223Combined sources3
Turni224 – 226Combined sources3
Beta strandi227 – 235Combined sources9
Beta strandi240 – 250Combined sources11
Helixi255 – 270Combined sources16
Helixi275 – 277Combined sources3
Beta strandi280 – 284Combined sources5
Helixi290 – 293Combined sources4
Beta strandi299 – 303Combined sources5
Helixi304 – 306Combined sources3
Beta strandi308 – 310Combined sources3
Beta strandi313 – 320Combined sources8
Helixi324 – 339Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EP7X-ray2.30A/B1-342[»]
ProteinModelPortaliO67161.
SMRiO67161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67161.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 153Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni210 – 211Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiO67161.
KOiK00134.
OMAiFVRVLSW.
OrthoDBiPOG091H02BL.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKVGINGF GRIGRSFFRA SWGREEIEIV AINDLTDAKH LAHLLKYDSV
60 70 80 90 100
HGIFKGSVEA KDDSIVVDGK EIKVFAQKDP SQIPWGDLGV DVVIEATGVF
110 120 130 140 150
RDRENASKHL QGGAKKVIIT APAKNPDITV VLGVNEEKYN PKEHNIISNA
160 170 180 190 200
SCTTNCLAPC VKVLNEAFGV EKGYMVTVHA YTNDQRLLDL PHKDFRRARA
210 220 230 240 250
AAINIVPTTT GAAKAIGEVI PELKGKLDGT ARRVPVPDGS LIDLTVVVNK
260 270 280 290 300
APSSVEEVNE KFREAAQKYR ESGKVYLKEI LQYCEDPIVS TDIVGNPHSA
310 320 330 340
IFDAPLTQVI DNLVHIAAWY DNEWGYSCRL RDLVIYLAER GL
Length:342
Mass (Da):37,613
Last modified:August 1, 1998 - v1
Checksum:i4A6E070088985DAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07122.1.
PIRiF70391.
RefSeqiNP_213724.1. NC_000918.1.
WP_010880662.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07122; AAC07122; aq_1065.
GeneIDi1193795.
KEGGiaae:aq_1065.
PATRICi20959410. VBIAquAeo85532_0825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07122.1.
PIRiF70391.
RefSeqiNP_213724.1. NC_000918.1.
WP_010880662.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EP7X-ray2.30A/B1-342[»]
ProteinModelPortaliO67161.
SMRiO67161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1065.

Proteomic databases

PRIDEiO67161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07122; AAC07122; aq_1065.
GeneIDi1193795.
KEGGiaae:aq_1065.
PATRICi20959410. VBIAquAeo85532_0825.

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiO67161.
KOiK00134.
OMAiFVRVLSW.
OrthoDBiPOG091H02BL.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Miscellaneous databases

EvolutionaryTraceiO67161.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_AQUAE
AccessioniPrimary (citable) accession number: O67161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.