Reviewed, UniProtKB/Swiss-Prot O67137 (GYRB_AQUAE)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:	gyrB
Ordered Locus Names:	aq_1026

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 792	792	DNA gyrase subunit B		PRO_0000145289

Sequences

	Sequence		Length	Mass (Da)
	O67137-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 07DC02DF293FF9F3	FASTA	792	90,530
	10 20 30 40 50 60 MKKRQSQTPQ EYTAEAIKAV SGLEHVRLRP AMYIGDIGER GLHHLIWEIL DNAVDEAVAG 70 80 90 100 110 120 YARNISVTIH RDNSVTVEDD GRGIPVDIHP ETGKPAVEMV FTMLGAGGKF EKKVYTYSGG 130 140 150 160 170 180 LHGVGASVVN ALSEWLIVEV YRDGKIYRMA FKRGEVVEPL HVVGETKKRG TKVSFKPDPE 190 200 210 220 230 240 IFETTEIKFD IVEKRVRELA YLNPEVKFEL TDERLGKHLI YKFDRGIEEL VKYLNEGKEP 250 260 270 280 290 300 LFKDIIRIQG EKEGVIVDIA FQYVKDYKER IESFVNNIKT VEGGTHVTGF RSGLSKAVIR 310 320 330 340 350 360 MAQGLKLAKE LKKSFTGEDV REGLTAVVAC KVPNPQFEGQ TKTKLGNQNV KQIVESITYD 370 380 390 400 410 420 FLTSYFEKKR DVLKAIVEKA IEAALAREAA KKAKELVRRK SPLEEGVLPG KLADCSETDP 430 440 450 460 470 480 SKCEIFLVEG DSAGGSAKQA RDRRYQAILP LRGKIINVEK ARIDKVLSND EIKAIVSALG 490 500 510 520 530 540 CGIGEDLDLK KLRYHKIILM TDADVDGSHI RTLLLTFFYR FMPKLVEEGY VYIAEPPLYR 550 560 570 580 590 600 VKKGKKEIYI KDDKEFEHFL LNEIREKGRL VDAREKEFKG EELVRLLIDL KDYEDAYRAL 610 620 630 640 650 660 VKSKGENLVN FLLTHRVREE DLRNPARVKE ITHLMEEELG DYRVDTKYNE LEGAYDIIFY 670 680 690 700 710 720 DDKLGTKTII DVNFLSSLSY REVLEGIHLH LPVQVFFENK KVEINSLGEI YDKFMDFARS 730 740 750 760 770 780 GMEVQRYKGL GEMNPEQLWE TTMNPKTRRL KKVKIEDAAE ADRIFTILMG EQVEPRREFI 790 EAYAKEVKHL DV

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07098.1.
PIR	F70388.
RefSeq	NP_213700.1.
3D structure databases
HSSP	HSSP built from PDB template 1KZN based on UniProtKB P06982.
ModBase	Search...
Genome annotation databases
GeneID	1193771.
GenomeReviews	Gene locus aq_1026 in contig AE000657_GR.
KEGG	aae:aq_1026.
NMPDR	fig|224324.1.peg.715.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67137.
OMA	GHLYIAQ.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1026-MON.
BRENDA	5.99.1.3. 189781.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR011557. GyrB. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR013506. Topo_IIA_B_2. IPR002288. Topo_IIA_B_C. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
ProDom	PD149633. DNA_gyrase_B. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01059. gyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_AQUAE

Accession

Primary (citable) accession number: O67137

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)