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Protein

Probable DNA double-strand break repair Rad50 ATPase

Gene

rad50

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in DNA double-strand break repair (DSBR). The Rad50/Mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific 3'-5' exonuclease activity. Rad50 provides an ATP-dependent control of Mre11 by unwinding and/or repositioning DNA ends into the Mre11 active site (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per homodimer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861ZincPROSITE-ProRule annotation
Metal bindingi489 – 4891ZincPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 398ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA double-strand break repair Rad50 ATPase
Gene namesi
Name:rad50
Ordered Locus Names:aq_1006
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 978978Probable DNA double-strand break repair Rad50 ATPasePRO_0000138669Add
BLAST

Interactioni

Subunit structurei

Homodimer. Forms a complex with Mre11 (By similarity).By similarity

Protein-protein interaction databases

STRINGi224324.aq_1006.

Structurei

3D structure databases

ProteinModelPortaliO67124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini440 – 542103Zinc-hookPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili160 – 421262Sequence analysisAdd
BLAST
Coiled coili440 – 47839Sequence analysisAdd
BLAST
Coiled coili510 – 54233Sequence analysisAdd
BLAST
Coiled coili578 – 826249Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi886 – 92439Ala/Asp-rich (DA-box)Add
BLAST

Domaini

The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer (By similarity).By similarity

Sequence similaritiesi

Belongs to the SMC family. RAD50 subfamily.Curated
Contains 1 zinc-hook domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105EYH. Bacteria.
COG0419. LUCA.
HOGENOMiHOG000154064.
InParanoidiO67124.
KOiK03546.
OMAiVISHIES.
OrthoDBiEOG6V7BNM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR027050. SbcC.
IPR004592. SbcC_gammaproteobac_type.
IPR013134. Zn_hook_RAD50.
[Graphical view]
PANTHERiPTHR32114. PTHR32114. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00618. sbcc. 1 hit.
PROSITEiPS51131. ZN_HOOK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLKLEVKG FTVYKKPQVI DFTPLKFFVI QGKTGAGKTS IIDAITYALY
60 70 80 90 100
GKVPRYGASV ATKYVLSRGE KELKVALDFS LRGRNYRVER YYREFPEDSQ
110 120 130 140 150
VRVYEEGRRL NIKANEVEKW LFKISGLDYK TFTKVILLPQ GEFDRFLKES
160 170 180 190 200
SERKKILINL LGLEELEKVR QLASETFKNL EGKREALKKE YELLKDYTPT
210 220 230 240 250
KKEVLEKTLK NLEEELKELK ETEEKLRQEL KKAEEKDSLE RELSQVVTKL
260 270 280 290 300
KELENLEKEV EKLREKLEFS RKVAPYVPIA KRIEEIDKKL TELKVRKNKL
310 320 330 340 350
TKELAVLKDE LSFAQEELNR IEAEKEKFKE EKEREKELEH RLKKLQEIKE
360 370 380 390 400
ILKELSQLSS SLKEKEREYE QAKQEFEDLS ERVEKGKKLV AETEEKLEKI
410 420 430 440 450
KELFSEEEYT SLKMKERLLV ELQRKLKELK EKEGQLENLT QKYKEKKKVH
460 470 480 490 500
EKVLNELKEL ERELKERELH YHAHMVASYL SPGDTCPVCG GIYRGKALEN
510 520 530 540 550
VDAEGISELK HAKELKEKEE REIDTTLKLY AQKINSLKEE MEKLRNEVEE
560 570 580 590 600
LRKEIPENLK ERIKKLEELR IEKEKLEHKL NKYRKALEDR QKQKEEAQAK
610 620 630 640 650
LHKAQTELEL LKEKIREKSR LVKEFKELYR VERLEDYEES LKEEINYINS
660 670 680 690 700
KLQEIEEKEK KLRKHFEELS SRKSKLEGEL SALNESINSL EEERKEKLKE
710 720 730 740 750
LANIYEVAKS PREVVELYLG DKEAELERKI KEFEESFQSL KLKKSEIEEK
760 770 780 790 800
LKEYEGIREL SDIKGEYESV KTQLEEKHKK LGEVKRELEH LGERLKRKEE
810 820 830 840 850
LQKEISELEK KLEVYRVISN DFRGDRFQKY VSEIMLQKVV DRASEYFYKF
860 870 880 890 900
TGNYFFELER ATKGRDKDIV VVESSTSQRR PVSSLSGGET FLASLSFAFA
910 920 930 940 950
VSDLLSGSAN LESLFIDEGF GSLDQDMRER VSEILEAIKT NVNKMIGIVS
960 970
HIPDFAERFT ERIVVEKKGD YSEVRVIY
Length:978
Mass (Da):115,898
Last modified:August 1, 1998 - v1
Checksum:i9B0F2BF51ADD1151
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07092.1.
PIRiA70387.
RefSeqiNP_213687.1. NC_000918.1.
WP_010880625.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07092; AAC07092; aq_1006.
GeneIDi1193758.
KEGGiaae:aq_1006.
PATRICi20959330. VBIAquAeo85532_0787.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07092.1.
PIRiA70387.
RefSeqiNP_213687.1. NC_000918.1.
WP_010880625.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07092; AAC07092; aq_1006.
GeneIDi1193758.
KEGGiaae:aq_1006.
PATRICi20959330. VBIAquAeo85532_0787.

Phylogenomic databases

eggNOGiENOG4105EYH. Bacteria.
COG0419. LUCA.
HOGENOMiHOG000154064.
InParanoidiO67124.
KOiK03546.
OMAiVISHIES.
OrthoDBiEOG6V7BNM.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-720-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR027050. SbcC.
IPR004592. SbcC_gammaproteobac_type.
IPR013134. Zn_hook_RAD50.
[Graphical view]
PANTHERiPTHR32114. PTHR32114. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00618. sbcc. 1 hit.
PROSITEiPS51131. ZN_HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiRAD50_AQUAE
AccessioniPrimary (citable) accession number: O67124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.