Reviewed, UniProtKB/Swiss-Prot O67108 (GYRA_AQUAE)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	aq_980

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	DNA gyrase subunit A		PRO_0000145219
Sites
	Active site	121	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67108-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: BB574AEC1C1AEEA4	FASTA	744	84,081
	10 20 30 40 50 60 MENRENLVEI PIEEEVKQAY IDYAMSVIVG RAIPDVRDGL KPVQRRILYS MYEMGLLPDK 70 80 90 100 110 120 PFKKSARIVG ETLGKYHPHG DQAVYEALVR MAQDFTMRYP LIIGQGNFGS IDGDPAAQMR 130 140 150 160 170 180 YTEAKLSPLA VEMLTDIDKD TVDFQPNFDD TLMEPEVLPS KFPNLLCNGT SGIAVGLATS 190 200 210 220 230 240 IPPHNLTEVG NALVKLAQNP QISVDEIMEI LKGPDFPTGG VIENFAQVKE IYKTGRGIIK 250 260 270 280 290 300 VKGKAHVEKV QGGRERIVIT EIPYQVNKAE LIKKIADNVR NGKIKEISDI RDETDKEGIR 310 320 330 340 350 360 IVVELKRDAK GEEVLKKLYK YTPLEKGFPV NLVVLIDKEP KLVDIKTLLR EFIKHRLEVI 370 380 390 400 410 420 LRRSKYFLKK VQDRLHIVEG LLKAINFIDD IIERIRRSKD ASEARNYLME EFGLSEKQAQ 430 440 450 460 470 480 AVLDLRLQRL TSLEREKLLE EEKELREKIE YYKKLVASEG ERIKVFIEET EELVKKYGDK 490 500 510 520 530 540 RRTFIGGVKE VKEGSITVAV LQDGSIIPVE ELPLEKAPVV NILRVPFTEG LFLVSNRGRV 550 560 570 580 590 600 YWIAGSQALQ GSKVSLKSRE EKIVGAFIRE KFGNRLLLAT KKGYVKKIPL AEFEYKAQGM 610 620 630 640 650 660 PIIKLTEGDE VVSIASSVDE THILLFTKKG RVARFSVREV PPSTPGARGV QGIKLEKNDE 670 680 690 700 710 720 TSGLRIWNGE PYLLVITAKG RVKKISHEEI PKTNRGVKGT EVSGTKDTLV DLIPIKEEVE 730 740 LLITTKNGKA FYGQNQPERH TTFY

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07064.1.
PIR	A70385.
RefSeq	NP_213671.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	1193742.
GenomeReviews	Gene locus aq_980 in contig AE000657_GR.
KEGG	aae:aq_980.
NMPDR	fig|224324.1.peg.686.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67108.
OMA	IKVFIEE.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_980-MON.
BRENDA	5.99.1.3. 189781.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 4 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_AQUAE

Accession

Primary (citable) accession number: O67108

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)