Reviewed, UniProtKB/Swiss-Prot O67085 (PHEA_AQUAE)

Names and origin

Protein names

Recommended name:
    P-protein
Including the following 2 domains:
    1- Recommended name:
            Chorismate mutase
                Short name=CM
              EC=5.4.99.5
    2- Recommended name:
            Prephenate dehydratase
                Short name=PDT
              EC=4.2.1.51

Gene names

Name:	pheA
Ordered Locus Names:	aq_951

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	Chorismate = prephenate. Prephenate = phenylpyruvate + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1. Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 ACT domain. Contains 1 chorismate mutase domain. Contains 1 prephenate dehydratase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro chorismate mutase activity Inferred from electronic annotation. Source: EC prephenate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	P-protein		PRO_0000119181
Regions
	Domain	1 – 92	92	Chorismate mutase
	Domain	93 – 267	175	Prephenate dehydratase
	Domain	278 – 354	77	ACT
	Region	268 – 362	95	Regulatory (Phe-binding)
Sites
	Site	260	1	Essential for prephenate dehydratase activity Potential

Sequences

	Sequence		Length	Mass (Da)
	O67085-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 97FBA945E126436E	FASTA	362	41,187
	10 20 30 40 50 60 MEELKELRKE IDRIDEEILR LLNERAKLAK RIGEIKSKAN LPIHVPERER EIFEKILRLN 70 80 90 100 110 120 KEVYGGVFPQ EALVHIYREI ISACLSLEKK IKVAYLGPKA TFTHQAALEF FGFSAHYTPC 130 140 150 160 170 180 STIRDVFVEV ETKRADYGVV PVENTIEGVV NYTLDMFLES DVKIAGEIVI PITLHLLSAS 190 200 210 220 230 240 DSIENVEKVY SHKMALAQCR SWLEKNLPSV QVIEVESTAK ACEIALEDER AGAVASEVAA 250 260 270 280 290 300 YTYHLNILAR NIQDSGDNFT RFLVIAKRDL KPTGSDKTSI LFGVKDEPGA LYKALEVFYK 310 320 330 340 350 360 HGINLTKIES RPSKKKAWDY VFFVDLEGHK EEERVEKALK ELKEKTQFLK VLGSYPKALL QE

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07041.1.
PIR	B70382.
RefSeq	NP_213648.1.
3D structure databases
HSSP	HSSP built from PDB template 1PHZ based on UniProtKB P04176.
ModBase	Search...
Genome annotation databases
GeneID	1193627.
GenomeReviews	Gene locus aq_951 in contig AE000657_GR.
KEGG	aae:aq_951.
NMPDR	fig|224324.1.peg.663.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67085.
OMA	O67085. TLYSHPQ.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_951-MON.
BRENDA	4.2.1.51. 189781. 5.4.99.5. 189781.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR008242. Chor_mutase/pphenate_deHydtase. IPR002701. Chorismate_mut. IPR010957. CM_P2. IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF01842. ACT. 1 hit. PF01817. CM_2. 1 hit. PF00800. PDT. 1 hit. [Graphical view]
PIRSF	PIRSF001500. Chor_mut_pdt_Ppr. 1 hit.
TIGRFAMs	TIGR01807. CM_P2. 1 hit.
PROSITE	PS51168. CHORISMATE_MUT_2. 1 hit. PS00857. PREPHENATE_DEHYDR_1. 1 hit. PS00858. PREPHENATE_DEHYDR_2. 1 hit. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEA_AQUAE

Accession

Primary (citable) accession number: O67085

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)