O67083 (HEMH_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	aq_948

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Ferrochelatase HAMAP MF_00323		PRO_0000175101
Sites
	Metal binding	185	1	Iron By similarity
	Metal binding	264	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67083 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: BC8C9295F07951E5	FASTA	309	36,037
	10 20 30 40 50 60 MKKGVILINL GGPDSLEAVE PFLYNLFSDP DIFSLPFQKV LAKIIAKLRA KKTRHYYELM 70 80 90 100 110 120 GGKSPQYEQT LEQAKALQER LGEDYKVVVG MRYWKPYIKD ALSELLKEGI NEVILLPLYP 130 140 150 160 170 180 QYSKTTTGSA FNEFERSKKA LKADHIKVKK IEHFYDHPLY IKAWAEQIKQ SVEKPEEYHF 190 200 210 220 230 240 LFSAHSLPKK LIEEGDPYQE QTEKTVKLIM ENFPEVEYTL AYQSKVGFGK WLEPSTDEVI 250 260 270 280 290 300 RNLIKKEVKK LLVIPISFVS EHSETLYELD KQYRELAQEL GYEEFVRVPT LRTNPYFISA LEDLVKNEV

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07043.1.
PIR	H70381.
RefSeq	NP_213646.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67083.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193625.
GenomeReviews	Gene locus aq_948 in contig AE000657_GR.
KEGG	aae:aq_948.
NMPDR	fig|224324.1.peg.661.
PATRIC	20959244. VBIAquAeo85532_0744.
Phylogenomic databases
HOGENOM	HBG697135.
OMA	LLQMGGP.
PhylomeDB	O67083.
ProtClustDB	CLSK2299560.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_948-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_AQUAE

Accession

Primary (citable) accession number: O67083

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program