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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi364Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi367Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:aq_940
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768541 – 4323-isopropylmalate dehydratase large subunitAdd BLAST432

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_940.

Structurei

3D structure databases

ProteinModelPortaliO67078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI. Bacteria.
COG0065. LUCA.
HOGENOMiHOG000226971.
InParanoidiO67078.
KOiK01703.
OMAiKIEPQVA.
OrthoDBiPOG091H02GD.

Family and domain databases

CDDicd01583. IPMI. 1 hit.
Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01027. LeuC_type2. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR011826. HAcnase/IPMdehydase_lsu_prok.
IPR006251. Homoacnase/IPMdehydase_lsu.
IPR033941. IPMI_cat.
IPR011823. IsopropMal_deHydtase_lsu_bac.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01343. hacA_fam. 1 hit.
TIGR02086. IPMI_arch. 1 hit.
TIGR02083. LEU2. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMTITEKIL AEHAGKKEVH PGELITAKID LAMANDVTAP LAIKILEKYG
60 70 80 90 100
IDKVFDPERI ALVLSHFVPA KDIKSAEQAK IVREFVKKHG IKWFFEEGEG
110 120 130 140 150
IEHAILPEQG LVVPGDLVVG ADSHTCTYGA LGAFATGVGS TDIAYAMATG
160 170 180 190 200
EVWLRVPESM KFIFYGKLKP WVMGKDLILY TIGQIGVDGA LYRAMEFDGE
210 220 230 240 250
TIRNLSMEQR FTIANMAIEA GGKSGIISPD EKTIEYVKQR AKRPWKVYQS
260 270 280 290 300
DPDAEYHSVY EWDASQIEPL VAWPYLPSNV HPVSESTHIT IDQAFIGSCT
310 320 330 340 350
NGRIEDLRVA AKVFEAALKQ GKKVHPYVRC IVIPASKAIY KQALKEGLID
360 370 380 390 400
IFMEAGCVVS TSTCGPCLGG HMGVLAEGER CISTSNRNFP GRMGHPKSES
410 420 430
YLANPAVVAA SAIMGRIAHP DEVVKAETLA GV
Length:432
Mass (Da):47,088
Last modified:August 1, 1998 - v1
Checksum:iB97BB38BF3323844
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07028.1.
PIRiC70381.
RefSeqiNP_213641.1. NC_000918.1.
WP_010880579.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07028; AAC07028; aq_940.
GeneIDi1193620.
KEGGiaae:aq_940.
PATRICi20959226. VBIAquAeo85532_0735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07028.1.
PIRiC70381.
RefSeqiNP_213641.1. NC_000918.1.
WP_010880579.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07028; AAC07028; aq_940.
GeneIDi1193620.
KEGGiaae:aq_940.
PATRICi20959226. VBIAquAeo85532_0735.

Phylogenomic databases

eggNOGiENOG4105CQI. Bacteria.
COG0065. LUCA.
HOGENOMiHOG000226971.
InParanoidiO67078.
KOiK01703.
OMAiKIEPQVA.
OrthoDBiPOG091H02GD.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.

Family and domain databases

CDDicd01583. IPMI. 1 hit.
Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01027. LeuC_type2. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR011826. HAcnase/IPMdehydase_lsu_prok.
IPR006251. Homoacnase/IPMdehydase_lsu.
IPR033941. IPMI_cat.
IPR011823. IsopropMal_deHydtase_lsu_bac.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01343. hacA_fam. 1 hit.
TIGR02086. IPMI_arch. 1 hit.
TIGR02083. LEU2. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEUC_AQUAE
AccessioniPrimary (citable) accession number: O67078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.