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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. Probable branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi364Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi367Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-668-MONOMER
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:aq_940
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000768541 – 4323-isopropylmalate dehydratase large subunitAdd BLAST432

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_940

Structurei

3D structure databases

ProteinModelPortaliO67078
SMRiO67078
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI Bacteria
COG0065 LUCA
HOGENOMiHOG000226971
InParanoidiO67078
KOiK01703
OMAiIEHCLLP
OrthoDBiPOG091H02GD

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01027 LeuC_type2, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR011826 HAcnase/IPMdehydase_lsu_prok
IPR006251 Homoacnase/IPMdehydase_lsu
IPR033941 IPMI_cat
IPR011823 IsopropMal_deHydtase_lsu_bac
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01343 hacA_fam, 1 hit
TIGR02086 IPMI_arch, 1 hit
TIGR02083 LEU2, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O67078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMTITEKIL AEHAGKKEVH PGELITAKID LAMANDVTAP LAIKILEKYG
60 70 80 90 100
IDKVFDPERI ALVLSHFVPA KDIKSAEQAK IVREFVKKHG IKWFFEEGEG
110 120 130 140 150
IEHAILPEQG LVVPGDLVVG ADSHTCTYGA LGAFATGVGS TDIAYAMATG
160 170 180 190 200
EVWLRVPESM KFIFYGKLKP WVMGKDLILY TIGQIGVDGA LYRAMEFDGE
210 220 230 240 250
TIRNLSMEQR FTIANMAIEA GGKSGIISPD EKTIEYVKQR AKRPWKVYQS
260 270 280 290 300
DPDAEYHSVY EWDASQIEPL VAWPYLPSNV HPVSESTHIT IDQAFIGSCT
310 320 330 340 350
NGRIEDLRVA AKVFEAALKQ GKKVHPYVRC IVIPASKAIY KQALKEGLID
360 370 380 390 400
IFMEAGCVVS TSTCGPCLGG HMGVLAEGER CISTSNRNFP GRMGHPKSES
410 420 430
YLANPAVVAA SAIMGRIAHP DEVVKAETLA GV
Length:432
Mass (Da):47,088
Last modified:August 1, 1998 - v1
Checksum:iB97BB38BF3323844
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07028.1
PIRiC70381
RefSeqiNP_213641.1, NC_000918.1
WP_010880579.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07028; AAC07028; aq_940
GeneIDi1193620
KEGGiaae:aq_940
PATRICifig|224324.8.peg.735

Similar proteinsi

Entry informationi

Entry nameiLEUC_AQUAE
AccessioniPrimary (citable) accession number: O67078
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health