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Protein

Ribonuclease PH

Gene

rph

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates.By similarity

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-663-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PH (EC:2.7.7.56)
Short name:
RNase PH
Alternative name(s):
tRNA nucleotidyltransferase
Gene namesi
Name:rph
Synonyms:rnpH
Ordered Locus Names:aq_924
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Ribonuclease PHPRO_0000139861Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_924.

Structurei

Secondary structure

1
255
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 185Combined sources
Beta strandi21 – 3212Combined sources
Beta strandi35 – 4511Combined sources
Helixi49 – 513Combined sources
Beta strandi58 – 658Combined sources
Beta strandi67 – 693Combined sources
Turni78 – 803Combined sources
Helixi85 – 10117Combined sources
Helixi104 – 1074Combined sources
Beta strandi111 – 12010Combined sources
Helixi125 – 14622Combined sources
Beta strandi149 – 1524Combined sources
Beta strandi159 – 1679Combined sources
Beta strandi170 – 1745Combined sources
Helixi177 – 1815Combined sources
Beta strandi184 – 1929Combined sources
Beta strandi197 – 20610Combined sources
Helixi211 – 23323Combined sources
Beta strandi236 – 2394Combined sources
Beta strandi242 – 2454Combined sources
Beta strandi249 – 2524Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UDNX-ray2.30A1-255[»]
1UDOX-ray2.30A1-255[»]
1UDQX-ray2.30A1-255[»]
1UDSX-ray2.30A1-255[»]
ProteinModelPortaliO67069.
SMRiO67069. Positions 2-255.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67069.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.Curated

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
InParanoidiO67069.
KOiK00989.
OMAiKGKGQGW.
OrthoDBiEOG6CZQQP.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSDGRKEDQ LRPVSIQRDF LEYPEGSCLI SFGKTKVICT ASVIENVPNW
60 70 80 90 100
LKGKGQGWIT AEYSMLPRAT QQRTIRESVQ GRIGGRTHEI QRMIGRAMRT
110 120 130 140 150
AVELTKIGER TIWVDCDVIQ ADGGTRTAAI TGAFVAVADA IIKLHKEGII
160 170 180 190 200
EETPIKDFVA AVSVGIVNDR ILLDLNFEED SAAQVDMNVV GTGSGRLSEV
210 220 230 240 250
HTMGEEYSFT KDELIKMLDL AQKGINELIE LQKKLYVIQD GKWERSELKE

VSSTT
Length:255
Mass (Da):28,372
Last modified:August 1, 1998 - v1
Checksum:i7131CCD8AEDC3917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07032.1.
PIRiB70380.
RefSeqiNP_213631.1. NC_000918.1.
WP_010880569.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07032; AAC07032; aq_924.
GeneIDi1193611.
KEGGiaae:aq_924.
PATRICi20959202. VBIAquAeo85532_0724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07032.1.
PIRiB70380.
RefSeqiNP_213631.1. NC_000918.1.
WP_010880569.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UDNX-ray2.30A1-255[»]
1UDOX-ray2.30A1-255[»]
1UDQX-ray2.30A1-255[»]
1UDSX-ray2.30A1-255[»]
ProteinModelPortaliO67069.
SMRiO67069. Positions 2-255.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_924.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07032; AAC07032; aq_924.
GeneIDi1193611.
KEGGiaae:aq_924.
PATRICi20959202. VBIAquAeo85532_0724.

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
InParanoidiO67069.
KOiK00989.
OMAiKGKGQGW.
OrthoDBiEOG6CZQQP.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-663-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO67069.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiRNPH_AQUAE
AccessioniPrimary (citable) accession number: O67069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.