Reviewed, UniProtKB/Swiss-Prot O67069 (RNPH_AQUAE)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Synonyms:	rnpH
Ordered Locus Names:	aq_924

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 255

255

Ribonuclease PH HAMAP MF_00564

PRO_0000139861

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

255

Helix Strand Turn

Details...

Beta strand

14 – 18

5

Beta strand

21 – 32

12

Beta strand

35 – 45

11

Helix

49 – 51

3

Beta strand

58 – 65

8

Helix

85 – 101

17

Helix

104 – 107

4

Beta strand

111 – 120

10

Helix

125 – 146

22

Beta strand

149 – 152

4

Beta strand

159 – 167

9

Beta strand

170 – 174

5

Helix

177 – 181

5

Beta strand

184 – 192

9

Beta strand

197 – 206

10

Helix

211 – 233

23

Beta strand

236 – 239

4

Beta strand

242 – 245

4

Beta strand

249 – 252

4

Sequences

	Sequence		Length	Mass (Da)
	O67069-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7131CCD8AEDC3917	FASTA	255	28,372
	10 20 30 40 50 60 MRSDGRKEDQ LRPVSIQRDF LEYPEGSCLI SFGKTKVICT ASVIENVPNW LKGKGQGWIT 70 80 90 100 110 120 AEYSMLPRAT QQRTIRESVQ GRIGGRTHEI QRMIGRAMRT AVELTKIGER TIWVDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI TGAFVAVADA IIKLHKEGII EETPIKDFVA AVSVGIVNDR ILLDLNFEED 190 200 210 220 230 240 SAAQVDMNVV GTGSGRLSEV HTMGEEYSFT KDELIKMLDL AQKGINELIE LQKKLYVIQD 250 GKWERSELKE VSSTT

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000657 Genomic DNA. Translation: AAC07032.1.

PIR

B70380.

RefSeq

NP_213631.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UDN	X-ray	2.30	A	1-255	[»]
1UDO	X-ray	2.30	A	1-255	[»]
1UDQ	X-ray	2.30	A	1-255	[»]
1UDS	X-ray	2.30	A	1-255	[»]

ModBase

Search...

Genome annotation databases

GeneID

1193611.

GenomeReviews

Gene locus aq_924 in contig AE000657_GR.

KEGG

aae:aq_924.

NMPDR

fig|224324.1.peg.646.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O67069.

OMA

YAMLPRA.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_924-MON.

BRENDA

2.7.7.56. 189781.

Family and domain databases

HAMAP

MF_00564.
[Tree]

InterPro

IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR018336. Ribonuclease-PH_CS.
IPR002381. RNase_PH_bac-type.
[Graphical view]

Pfam

PF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01966. RNasePH. 1 hit.

PROSITE

PS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RNPH_AQUAE

Accession

Primary (citable) accession number: O67069

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)