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Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9UniRule annotation1
Active sitei130UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 98ATPUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.148. 396.
UniPathwayiUPA00056; UER00094.

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:aq_915
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001891831 – 2684-diphosphocytidyl-2-C-methyl-D-erythritol kinaseAdd BLAST268

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_915.

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 19Combined sources18
Beta strandi25 – 46Combined sources22
Beta strandi48 – 53Combined sources6
Turni57 – 59Combined sources3
Helixi61 – 73Combined sources13
Beta strandi79 – 84Combined sources6
Beta strandi91 – 93Combined sources3
Helixi95 – 110Combined sources16
Helixi117 – 127Combined sources11
Turni129 – 131Combined sources3
Helixi132 – 135Combined sources4
Beta strandi138 – 143Combined sources6
Turni144 – 147Combined sources4
Beta strandi148 – 151Combined sources4
Beta strandi158 – 164Combined sources7
Helixi171 – 176Combined sources6
Helixi180 – 182Combined sources3
Helixi186 – 197Combined sources12
Helixi201 – 203Combined sources3
Helixi207 – 215Combined sources9
Helixi217 – 228Combined sources12
Beta strandi242 – 246Combined sources5
Helixi250 – 259Combined sources10
Beta strandi262 – 268Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V2QX-ray2.30A/B1-268[»]
2V2VX-ray2.40A/B1-268[»]
2V2ZX-ray2.25A/B1-268[»]
2V34X-ray2.30A/B1-268[»]
2V8PX-ray2.10A/B1-268[»]
2VF3X-ray2.20A/B1-268[»]
ProteinModelPortaliO67060.
SMRiO67060.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67060.

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTR. Bacteria.
COG1947. LUCA.
HOGENOMiHOG000019600.
InParanoidiO67060.
KOiK00919.
OMAiFFLHGGC.
OrthoDBiPOG091H00XD.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.

Sequencei

Sequence statusi: Complete.

O67060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVLSPAKI NLGLWVLGRL PSGYHEILTL YQEIPFYDEI YIREGVLRVE
60 70 80 90 100
TNIGIPQEEN LVYKGLREFE RITGIEINYS IFIQKNIPPG AGLGGGSSNL
110 120 130 140 150
AVVLKKVNEL LGSPLSEEEL RELVGSISAD APFFLLGKSA IGRGKGEVLE
160 170 180 190 200
PVETEISGKI TLVIPQVSSS TGRVYSSLRE EHFVTPEYAE EKIQRIISGE
210 220 230 240 250
VEEIENVLGD IARELYPEIN EVYRFVEYLG FKPFVSGSGS TVYFFGGASE
260
ELKKAAKMRG WKVVELEL
Length:268
Mass (Da):29,788
Last modified:August 1, 1998 - v1
Checksum:iC905F9B988E0B45D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07027.1.
PIRiA70379.
RefSeqiNP_213622.1. NC_000918.1.
WP_010880560.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07027; AAC07027; aq_915.
GeneIDi1193602.
KEGGiaae:aq_915.
PATRICi20959182. VBIAquAeo85532_0714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07027.1.
PIRiA70379.
RefSeqiNP_213622.1. NC_000918.1.
WP_010880560.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V2QX-ray2.30A/B1-268[»]
2V2VX-ray2.40A/B1-268[»]
2V2ZX-ray2.25A/B1-268[»]
2V34X-ray2.30A/B1-268[»]
2V8PX-ray2.10A/B1-268[»]
2VF3X-ray2.20A/B1-268[»]
ProteinModelPortaliO67060.
SMRiO67060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07027; AAC07027; aq_915.
GeneIDi1193602.
KEGGiaae:aq_915.
PATRICi20959182. VBIAquAeo85532_0714.

Phylogenomic databases

eggNOGiENOG4105CTR. Bacteria.
COG1947. LUCA.
HOGENOMiHOG000019600.
InParanoidiO67060.
KOiK00919.
OMAiFFLHGGC.
OrthoDBiPOG091H00XD.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094.
BRENDAi2.7.1.148. 396.

Miscellaneous databases

EvolutionaryTraceiO67060.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE. 1 hit.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPE_AQUAE
AccessioniPrimary (citable) accession number: O67060
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.