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Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91UniRule annotation
Active sitei130 – 1301UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 9811ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-654-MONOMER.
BRENDAi2.7.1.148. 396.
UniPathwayiUPA00056; UER00094.

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:aq_915
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2682684-diphosphocytidyl-2-C-methyl-D-erythritol kinasePRO_0000189183Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_915.

Structurei

Secondary structure

1
268
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1918Combined sources
Beta strandi25 – 4622Combined sources
Beta strandi48 – 536Combined sources
Turni57 – 593Combined sources
Helixi61 – 7313Combined sources
Beta strandi79 – 846Combined sources
Beta strandi91 – 933Combined sources
Helixi95 – 11016Combined sources
Helixi117 – 12711Combined sources
Turni129 – 1313Combined sources
Helixi132 – 1354Combined sources
Beta strandi138 – 1436Combined sources
Turni144 – 1474Combined sources
Beta strandi148 – 1514Combined sources
Beta strandi158 – 1647Combined sources
Helixi171 – 1766Combined sources
Helixi180 – 1823Combined sources
Helixi186 – 19712Combined sources
Helixi201 – 2033Combined sources
Helixi207 – 2159Combined sources
Helixi217 – 22812Combined sources
Beta strandi242 – 2465Combined sources
Helixi250 – 25910Combined sources
Beta strandi262 – 2687Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V2QX-ray2.30A/B1-268[»]
2V2VX-ray2.40A/B1-268[»]
2V2ZX-ray2.25A/B1-268[»]
2V34X-ray2.30A/B1-268[»]
2V8PX-ray2.10A/B1-268[»]
2VF3X-ray2.20A/B1-268[»]
ProteinModelPortaliO67060.
SMRiO67060. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67060.

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTR. Bacteria.
COG1947. LUCA.
HOGENOMiHOG000019600.
InParanoidiO67060.
KOiK00919.
OMAiRAHISIG.
OrthoDBiEOG62VNQ2.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.

Sequencei

Sequence statusi: Complete.

O67060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVLSPAKI NLGLWVLGRL PSGYHEILTL YQEIPFYDEI YIREGVLRVE
60 70 80 90 100
TNIGIPQEEN LVYKGLREFE RITGIEINYS IFIQKNIPPG AGLGGGSSNL
110 120 130 140 150
AVVLKKVNEL LGSPLSEEEL RELVGSISAD APFFLLGKSA IGRGKGEVLE
160 170 180 190 200
PVETEISGKI TLVIPQVSSS TGRVYSSLRE EHFVTPEYAE EKIQRIISGE
210 220 230 240 250
VEEIENVLGD IARELYPEIN EVYRFVEYLG FKPFVSGSGS TVYFFGGASE
260
ELKKAAKMRG WKVVELEL
Length:268
Mass (Da):29,788
Last modified:August 1, 1998 - v1
Checksum:iC905F9B988E0B45D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07027.1.
PIRiA70379.
RefSeqiNP_213622.1. NC_000918.1.
WP_010880560.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07027; AAC07027; aq_915.
GeneIDi1193602.
KEGGiaae:aq_915.
PATRICi20959182. VBIAquAeo85532_0714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07027.1.
PIRiA70379.
RefSeqiNP_213622.1. NC_000918.1.
WP_010880560.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V2QX-ray2.30A/B1-268[»]
2V2VX-ray2.40A/B1-268[»]
2V2ZX-ray2.25A/B1-268[»]
2V34X-ray2.30A/B1-268[»]
2V8PX-ray2.10A/B1-268[»]
2VF3X-ray2.20A/B1-268[»]
ProteinModelPortaliO67060.
SMRiO67060. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07027; AAC07027; aq_915.
GeneIDi1193602.
KEGGiaae:aq_915.
PATRICi20959182. VBIAquAeo85532_0714.

Phylogenomic databases

eggNOGiENOG4105CTR. Bacteria.
COG1947. LUCA.
HOGENOMiHOG000019600.
InParanoidiO67060.
KOiK00919.
OMAiRAHISIG.
OrthoDBiEOG62VNQ2.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00094.
BioCyciAAEO224324:GJBH-654-MONOMER.
BRENDAi2.7.1.148. 396.

Miscellaneous databases

EvolutionaryTraceiO67060.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00061. IspE.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF010376. IspE. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00154. ispE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiISPE_AQUAE
AccessioniPrimary (citable) accession number: O67060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.