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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation1 Publication

Miscellaneous

AroE is dependent on the hydrogen ion concentration of the environment. A low pH environment favors the formation of SA and NADP, whereas a high pH condition favors the formation of DHSA and NADPH.1 Publication

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation1 Publication

Kineticsi

Kcat is 55.5 sec(-1) for dehydrogenase activity (at pH 9 and 25 degrees Celsius).1 Publication
  1. KM=42.4 µM for shikimate (at pH 9 and 25 degrees Celsius)1 Publication
  2. KM=42.4 µM for NADP (at pH 9 and 25 degrees Celsius)1 Publication

    Pathwayi: chorismate biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
    Proteins known to be involved in the 7 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. no protein annotated in this organism
    3. 3-dehydroquinate dehydratase (aroD)
    4. Shikimate dehydrogenase (NADP(+)) (aroE)
    5. Shikimate kinase (aroK)
    6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
    7. Chorismate synthase (aroC)
    This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei66ShikimateUniRule annotation1
    Active sitei70Proton acceptorUniRule annotation1 Publication1
    Binding sitei82NADPUniRule annotation1 Publication1
    Binding sitei91ShikimateUniRule annotation1 Publication1
    Binding sitei106ShikimateUniRule annotation1 Publication1
    Binding sitei214NADP; via carbonyl oxygenUniRule annotation1 Publication1
    Binding sitei216ShikimateUniRule annotation1 Publication1
    Binding sitei235NADP; via carbonyl oxygenUniRule annotation1 Publication1
    Binding sitei242ShikimateUniRule annotation1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi130 – 134NADPUniRule annotation1 Publication5
    Nucleotide bindingi153 – 158NADPUniRule annotation1 Publication6

    GO - Molecular functioni

    • NADP binding Source: UniProtKB
    • shikimate 3-dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    • aromatic amino acid family biosynthetic process Source: UniProtKB-KW
    • chorismate biosynthetic process Source: UniProtKB
    • shikimate metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
    LigandNADP

    Enzyme and pathway databases

    BioCyciAAEO224324:G1G15-637-MONOMER
    BRENDAi1.1.1.25 396
    UniPathwayiUPA00053; UER00087

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shikimate dehydrogenase (NADP(+))1 PublicationUniRule annotation (EC:1.1.1.25UniRule annotation1 Publication)
    Short name:
    SD1 Publication
    Short name:
    SDH1 PublicationUniRule annotation
    Gene namesi
    Name:aroE1 PublicationUniRule annotation
    Ordered Locus Names:aq_901
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    Proteomesi
    • UP000000798 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001359901 – 269Shikimate dehydrogenase (NADP(+))Add BLAST269

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi224324.aq_901

    Structurei

    Secondary structure

    1269
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 15Combined sources9
    Helixi21 – 32Combined sources12
    Beta strandi35 – 42Combined sources8
    Helixi45 – 47Combined sources3
    Helixi48 – 58Combined sources11
    Beta strandi62 – 65Combined sources4
    Turni70 – 73Combined sources4
    Helixi74 – 76Combined sources3
    Beta strandi78 – 80Combined sources3
    Helixi82 – 87Combined sources6
    Beta strandi92 – 96Combined sources5
    Beta strandi99 – 103Combined sources5
    Helixi106 – 117Combined sources12
    Helixi121 – 123Combined sources3
    Beta strandi124 – 129Combined sources6
    Helixi133 – 145Combined sources13
    Beta strandi148 – 152Combined sources5
    Helixi156 – 162Combined sources7
    Turni163 – 165Combined sources3
    Beta strandi168 – 170Combined sources3
    Helixi174 – 176Combined sources3
    Helixi178 – 180Combined sources3
    Beta strandi182 – 186Combined sources5
    Helixi202 – 204Combined sources3
    Beta strandi209 – 217Combined sources9
    Helixi220 – 227Combined sources8
    Beta strandi231 – 233Combined sources3
    Helixi236 – 251Combined sources16
    Helixi257 – 268Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HK7X-ray2.50A/B1-269[»]
    2HK8X-ray2.35A/B/C/D/E/F/G/H1-269[»]
    2HK9X-ray2.20A/B/C/D1-269[»]
    ProteinModelPortaliO67049
    SMRiO67049
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO67049

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni19 – 21Shikimate bindingUniRule annotation1 Publication3

    Sequence similaritiesi

    Belongs to the shikimate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105E2X Bacteria
    COG0169 LUCA
    HOGENOMiHOG000237875
    InParanoidiO67049
    KOiK00014
    OMAiFGNPIKH
    OrthoDBiPOG091H02DY

    Family and domain databases

    HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR011342 Shikimate_DH
    IPR013708 Shikimate_DH-bd_N
    IPR022893 Shikimate_DH_fam
    IPR006151 Shikm_DH/Glu-tRNA_Rdtase
    PfamiView protein in Pfam
    PF01488 Shikimate_DH, 1 hit
    PF08501 Shikimate_dh_N, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR00507 aroE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O67049-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MINAQTQLYG VIGFPVKHSL SPVFQNALIR YAGLNAVYLA FEINPEELKK
    60 70 80 90 100
    AFEGFKALKV KGINVTVPFK EEIIPLLDYV EDTAKEIGAV NTVKFENGKA
    110 120 130 140 150
    YGYNTDWIGF LKSLKSLIPE VKEKSILVLG AGGASRAVIY ALVKEGAKVF
    160 170 180 190 200
    LWNRTKEKAI KLAQKFPLEV VNSPEEVIDK VQVIVNTTSV GLKDKDPEIF
    210 220 230 240 250
    NYDLIKKDHV VVDIIYKETK LLKKAKEKGA KLFDGLPMLL WQGIEAFKIW
    260
    NGCEVPYSVA ERSVRDLRG
    Length:269
    Mass (Da):30,106
    Last modified:August 1, 1998 - v1
    Checksum:i3F2E5E490FB9ADB7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA Translation: AAC07007.1
    PIRiF70377
    RefSeqiNP_213611.1, NC_000918.1
    WP_010880549.1, NC_000918.1

    Genome annotation databases

    EnsemblBacteriaiAAC07007; AAC07007; aq_901
    GeneIDi1193591
    KEGGiaae:aq_901
    PATRICifig|224324.8.peg.703

    Entry informationi

    Entry nameiAROE_AQUAE
    AccessioniPrimary (citable) accession number: O67049
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: March 28, 2018
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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