Reviewed, UniProtKB/Swiss-Prot O67049 (AROE_AQUAE)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	aq_901

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 269

269

Shikimate dehydrogenase HAMAP MF_00222

PRO_0000135990

Regions

Nucleotide binding

130 – 134

5

NADP By similarity

Sites

Active site

70

1

Proton acceptor Potential

Secondary structure

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269

Helix Strand Turn

Details...

Beta strand

7 – 9

3

Helix

21 – 31

11

Beta strand

36 – 38

3

Helix

45 – 47

3

Helix

48 – 52

5

Turn

56 – 59

4

Turn

70 – 73

4

Helix

74 – 76

3

Helix

82 – 86

5

Helix

111 – 117

7

Helix

121 – 123

3

Beta strand

124 – 129

6

Helix

135 – 144

10

Beta strand

148 – 151

4

Helix

156 – 162

7

Turn

163 – 165

3

Beta strand

168 – 170

3

Helix

174 – 176

3

Helix

178 – 180

3

Beta strand

182 – 186

5

Helix

202 – 204

3

Beta strand

209 – 217

9

Helix

220 – 227

8

Beta strand

231 – 233

3

Helix

237 – 241

5

Helix

245 – 250

6

Helix

257 – 264

8

Turn

265 – 267

3

Sequences

	Sequence		Length	Mass (Da)
	O67049-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 3F2E5E490FB9ADB7	FASTA	269	30,106
	10 20 30 40 50 60 MINAQTQLYG VIGFPVKHSL SPVFQNALIR YAGLNAVYLA FEINPEELKK AFEGFKALKV 70 80 90 100 110 120 KGINVTVPFK EEIIPLLDYV EDTAKEIGAV NTVKFENGKA YGYNTDWIGF LKSLKSLIPE 130 140 150 160 170 180 VKEKSILVLG AGGASRAVIY ALVKEGAKVF LWNRTKEKAI KLAQKFPLEV VNSPEEVIDK 190 200 210 220 230 240 VQVIVNTTSV GLKDKDPEIF NYDLIKKDHV VVDIIYKETK LLKKAKEKGA KLFDGLPMLL 250 260 WQGIEAFKIW NGCEVPYSVA ERSVRDLRG

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000657 Genomic DNA. Translation: AAC07007.1.

PIR

F70377.

RefSeq

NP_213611.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HK7	X-ray	2.50	A/B	1-269	[»]
2HK8	X-ray	2.35	A/B/C/D/E/F/G/H	1-269	[»]
2HK9	X-ray	2.20	A/B/C/D	1-269	[»]

ModBase

Search...

Genome annotation databases

GeneID

1193591.

GenomeReviews

Gene locus aq_901 in contig AE000657_GR.

KEGG

aae:aq_901.

NMPDR

fig|224324.1.peg.626.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O67049.

OMA

DAPGFLE.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_901-MON.

BRENDA

1.1.1.25. 189781.

Family and domain databases

HAMAP

MF_00222.
[Tree]

InterPro

IPR011342. Quinate/shikimate_5-DH.
IPR013708. Shikimate_DH-bd_N.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]

Pfam

PF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00507. aroE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

AROE_AQUAE

Accession

Primary (citable) accession number: O67049

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)