O67036 (DXS_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	aq_881

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189082

Sequences

	Sequence		Length	Mass (Da)
	O67036 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7253218092B81B9A	FASTA	628	69,926
	10 20 30 40 50 60 MLEKYEILKD YKGPFDIKNY DYETLQKLAQ EVRDYIINVT SKNGGHVGPS LGVVELTIAL 70 80 90 100 110 120 LRVFNPPEDV IVWDIGHQGY PWKILTDRKE QFPTLRQYKG ISGFLRREES IYDAFGAGHS 130 140 150 160 170 180 STSISAALGF RIGKDLKGEK EDYVIAVIGD GALTAGMAYE ALNNAGHIRP DRFIVILNDN 190 200 210 220 230 240 EMSISPNVGA ISTYLNRIIS GHFVQETRQK IKNFLQHFGE TPLRIMKLTE EFLKGLISPG 250 260 270 280 290 300 VIFEELGFNY IGPIDGHDIK ALEDTLNNVK DIKGPVLLHV YTKKGKGYKP AEENPVKWHG 310 320 330 340 350 360 VAPYKVESGE IIKKSSPPTW TSVFGKALVE LAERDEKIVA ITPAMREGSG LVEFAKRFPD 370 380 390 400 410 420 RFFDVGIAEQ HACTFAAGLA AEGLRPVAAY YSTFLQRAYD QVIHDVALQN LPVTFAIDRA 430 440 450 460 470 480 GLVGDDGPTH HGVFDLSYLR CVPNMVVCAP KDEQELRDLL YTGIYSGKPF ALRYPRGAAY 490 500 510 520 530 540 GVPTEGFKKI EIGTWEELLE GEDCVILAVG YPVYQALRAA EKLYKEGIRV GVVNARFVKP 550 560 570 580 590 600 MDEKMLRDLA NRYDTFITVE DNTVVGGFGS GVLEFFAREG IMKRVINLGV PDRFIEHGKQ 610 620 DILRNLVGID AEGIEKAVRD ALKGGRLI

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07004.1.
PIR	A70376.
RefSeq	NP_213598.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67036.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193578.
GenomeReviews	Gene locus aq_881 in contig AE000657_GR.
KEGG	aae:aq_881.
NMPDR	fig|224324.1.peg.613.
PATRIC	20959126. VBIAquAeo85532_0687.
Phylogenomic databases
HOGENOM	HBG571647.
OMA	SCERELM.
PhylomeDB	O67036.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_881-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AQUAE

Accession

Primary (citable) accession number: O67036

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program