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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

Miscellaneous

The active site is located at the dimer interface.By similarity

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.By similarity

Cofactori

a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132SubstrateBy similarity1
Metal bindingi161Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi205Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi258Divalent metal cation; shared with dimeric partnerBy similarity1
Binding sitei266SubstrateBy similarity1
Binding sitei275SubstrateBy similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandMetal-binding, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseBy similarity (EC:1.1.1.262By similarity)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseBy similarity
Gene namesi
Name:pdxABy similarity
Ordered Locus Names:aq_852
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887961 – 3204-hydroxythreonine-4-phosphate dehydrogenaseAdd BLAST320

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_852.

Structurei

3D structure databases

ProteinModelPortaliO67019.
SMRiO67019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.Curated

Phylogenomic databases

eggNOGiENOG4105CEZ. Bacteria.
COG1995. LUCA.
HOGENOMiHOG000221591.
InParanoidiO67019.
KOiK00097.
OMAiAPVHKGV.
OrthoDBiPOG091H03XD.

Family and domain databases

InterProiView protein in InterPro
IPR005255. PdxA.
PANTHERiPTHR30004. PTHR30004. 1 hit.
PfamiView protein in Pfam
PF04166. PdxA. 1 hit.
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

O67019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKIGITLG DPAGIGPELI LKISKHFKEK FTYVIYGEEK TLLEASKLTG
60 70 80 90 100
IKLNYKKIEK VEEAKERGVY LIDLNVLKVP VVEPSVSSGK AAVAYLARAV
110 120 130 140 150
ADAIRGNIHG ILTMPINKFW AKKAGFQYEG QTEFLAKASG TKDYAMMMYS
160 170 180 190 200
EKLKVVLLTT HIPLKDVPNY VKKEEILKKV RLIRKEFLEK FKFEPLIKVL
210 220 230 240 250
GLNPHAGEMG ELGREEIEEI IPAVEEAKKE GIKVVGPLVP DVAFINPSEE
260 270 280 290 300
DVFLCMYHDQ GLIPFKMLAF DEGVNFTLGL PFIRTSPDHG TAYDIAWKNK
310 320
ARESSSLHAL RLIEDLLDKI
Length:320
Mass (Da):35,914
Last modified:August 1, 1998 - v1
Checksum:i9A31DCECB3EA0AE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06973.1.
PIRiH70373.
RefSeqiNP_213580.1. NC_000918.1.
WP_010880518.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06973; AAC06973; aq_852.
GeneIDi1193561.
KEGGiaae:aq_852.
PATRICifig|224324.8.peg.667.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPDXA_AQUAE
AccessioniPrimary (citable) accession number: O67019
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: August 1, 1998
Last modified: June 7, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families