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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Mn2+By similarity

Pathway: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GDP.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT)
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GDP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-603-MONOMER.
UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:aq_844
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 696696Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000166567Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_844.

Structurei

3D structure databases

ProteinModelPortaliO67012.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 14597HDAdd
BLAST
Domaini623 – 69674ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
InParanoidiO67012.
KOiK01139.
OMAiLDWLNFV.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLGEVSLE EDLEKLLSHY PQHAEEIQRA YEFAKEKHGE QKRKTGEPYI
60 70 80 90 100
IHPLNVALKL AELGMDHETI IAALLHDTLE DTDTTYEEIK ERFGERVAKL
110 120 130 140 150
VEGVTKIGKI KYKSEQAENY RKLILATAED PRVILLKLSD RLDNVKTLWV
160 170 180 190 200
FREEKRKKIA KETMEIYAPL AHRLGVWSIK NELEDWAFKY LYPEEYEKVR
210 220 230 240 250
NFVKESRKNL EEYLRKYVIP KVRKELEKYG IEAEIKYRSK HYYSIWEKTR
260 270 280 290 300
RKGIRLEDVH DILGVRIIVN TVPECYTVLG IIHSLFRPVP GKFKDYISLP
310 320 330 340 350
KPNLYQSLHT TVIADKGKLV EFQIRTWEMH ERAEKGIASH WAYKEGKNPS
360 370 380 390 400
DAGVYSWLRE LVESIQGSTN PSEVLENLKS NLFFEEVFVF TPKGDLVVLP
410 420 430 440 450
KGSTPVDLAY KIHTEVGNHC AGAKSNGRIV PLNYELKSGD VVEIITNPNK
460 470 480 490 500
SPSYEWLSFV KTSRARNKIK QFLKKQERER YLSEGKRILE RIREKLGLSH
510 520 530 540 550
EDLINKIRER VRFDTEEELL LALGKRKISS ANLIKLIFPK KKEEKEERRG
560 570 580 590 600
SSTVFLEDLS NIKHEVAKCC KPIPGDEILG VITRTKGLVL HEKSCSNLKN
610 620 630 640 650
VLRLNPEKVK EVQLQASGYF QTDIRVVASD RIGLLSDITK VISESGSNIV
660 670 680 690
SSMTNTREGK AVMDFTVEVK NKEHLEKIMK KIKSVEGVKI CKRLYH
Length:696
Mass (Da):80,570
Last modified:August 1, 1998 - v1
Checksum:i825F3FE6A556FF15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06975.1.
PIRiA70373.
RefSeqiNP_213573.1. NC_000918.1.
WP_010880511.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06975; AAC06975; aq_844.
GeneIDi1193554.
KEGGiaae:aq_844.
PATRICi20959066. VBIAquAeo85532_0658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06975.1.
PIRiA70373.
RefSeqiNP_213573.1. NC_000918.1.
WP_010880511.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_844.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06975; AAC06975; aq_844.
GeneIDi1193554.
KEGGiaae:aq_844.
PATRICi20959066. VBIAquAeo85532_0658.

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
InParanoidiO67012.
KOiK01139.
OMAiLDWLNFV.
OrthoDBiEOG6SV551.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.
BioCyciAAEO224324:GJBH-603-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiSPOT_AQUAE
AccessioniPrimary (citable) accession number: O67012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 27, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.