O67012 (SPOT_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
EC=3.1.7.2

Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name=(ppGpp)ase

Gene names

Name:	spoT
Ordered Locus Names:	aq_844

Organism

Aquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Aquifex › Aquifex aeolicus

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp By similarity.
Catalytic activity	Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.
Cofactor	Manganese By similarity.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	Manganese
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	amino acid binding Inferred from electronic annotation. Source: InterPro guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 696	696	Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase		PRO_0000166567
Regions
	Domain	49 – 145	97	HD
	Domain	622 – 694	73	ACT

Sequences

	Sequence		Length	Mass (Da)
	O67012 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 825F3FE6A556FF15	FASTA	696	80,570
	10 20 30 40 50 60 MSKLGEVSLE EDLEKLLSHY PQHAEEIQRA YEFAKEKHGE QKRKTGEPYI IHPLNVALKL 70 80 90 100 110 120 AELGMDHETI IAALLHDTLE DTDTTYEEIK ERFGERVAKL VEGVTKIGKI KYKSEQAENY 130 140 150 160 170 180 RKLILATAED PRVILLKLSD RLDNVKTLWV FREEKRKKIA KETMEIYAPL AHRLGVWSIK 190 200 210 220 230 240 NELEDWAFKY LYPEEYEKVR NFVKESRKNL EEYLRKYVIP KVRKELEKYG IEAEIKYRSK 250 260 270 280 290 300 HYYSIWEKTR RKGIRLEDVH DILGVRIIVN TVPECYTVLG IIHSLFRPVP GKFKDYISLP 310 320 330 340 350 360 KPNLYQSLHT TVIADKGKLV EFQIRTWEMH ERAEKGIASH WAYKEGKNPS DAGVYSWLRE 370 380 390 400 410 420 LVESIQGSTN PSEVLENLKS NLFFEEVFVF TPKGDLVVLP KGSTPVDLAY KIHTEVGNHC 430 440 450 460 470 480 AGAKSNGRIV PLNYELKSGD VVEIITNPNK SPSYEWLSFV KTSRARNKIK QFLKKQERER 490 500 510 520 530 540 YLSEGKRILE RIREKLGLSH EDLINKIRER VRFDTEEELL LALGKRKISS ANLIKLIFPK 550 560 570 580 590 600 KKEEKEERRG SSTVFLEDLS NIKHEVAKCC KPIPGDEILG VITRTKGLVL HEKSCSNLKN 610 620 630 640 650 660 VLRLNPEKVK EVQLQASGYF QTDIRVVASD RIGLLSDITK VISESGSNIV SSMTNTREGK 670 680 690 AVMDFTVEVK NKEHLEKIMK KIKSVEGVKI CKRLYH

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06975.1.
PIR	A70373.
RefSeq	NP_213573.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67012.
ModBase	Search...
Protein-protein interaction databases
STRING	224324.aq_844.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC06975; AAC06975; aq_844.
GeneID	1193554.
KEGG	aae:aq_844.
PATRIC	20959066. VBIAquAeo85532_0658.
Phylogenomic databases
eggNOG	COG0317.
HOGENOM	HOG000018299.
KO	K01139.
OMA	CVGLESY.
ProtClustDB	CLSK2299520.
Enzyme and pathway databases
BioCyc	AAEO224324:GJBH-603-MONOMER.
UniPathway	UPA00908; UER00886.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR002912. ACT_dom. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF01966. HD. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPOT_AQUAE

Accession

Primary (citable) accession number: O67012

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program