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O67012

- SPOT_AQUAE

UniProt

O67012 - SPOT_AQUAE

Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp By similarity.By similarity

    Catalytic activityi

    Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

    Cofactori

    Manganese.By similarity

    Pathwayi

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: UniProtKB-EC
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: InterPro

    GO - Biological processi

    1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-603-MONOMER.
    UniPathwayiUPA00908; UER00886.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
    Alternative name(s):
    Penta-phosphate guanosine-3'-pyrophosphohydrolase
    Short name:
    (ppGpp)ase
    Gene namesi
    Name:spoT
    Ordered Locus Names:aq_844
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 696696Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000166567Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224324.aq_844.

    Structurei

    3D structure databases

    ProteinModelPortaliO67012.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 14597HDAdd
    BLAST
    Domaini623 – 69674ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RelA/SpoT family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG0317.
    HOGENOMiHOG000018299.
    KOiK01139.
    OMAiIGYITRG.
    OrthoDBiEOG6SV551.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF01966. HD. 1 hit.
    PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67012-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKLGEVSLE EDLEKLLSHY PQHAEEIQRA YEFAKEKHGE QKRKTGEPYI    50
    IHPLNVALKL AELGMDHETI IAALLHDTLE DTDTTYEEIK ERFGERVAKL 100
    VEGVTKIGKI KYKSEQAENY RKLILATAED PRVILLKLSD RLDNVKTLWV 150
    FREEKRKKIA KETMEIYAPL AHRLGVWSIK NELEDWAFKY LYPEEYEKVR 200
    NFVKESRKNL EEYLRKYVIP KVRKELEKYG IEAEIKYRSK HYYSIWEKTR 250
    RKGIRLEDVH DILGVRIIVN TVPECYTVLG IIHSLFRPVP GKFKDYISLP 300
    KPNLYQSLHT TVIADKGKLV EFQIRTWEMH ERAEKGIASH WAYKEGKNPS 350
    DAGVYSWLRE LVESIQGSTN PSEVLENLKS NLFFEEVFVF TPKGDLVVLP 400
    KGSTPVDLAY KIHTEVGNHC AGAKSNGRIV PLNYELKSGD VVEIITNPNK 450
    SPSYEWLSFV KTSRARNKIK QFLKKQERER YLSEGKRILE RIREKLGLSH 500
    EDLINKIRER VRFDTEEELL LALGKRKISS ANLIKLIFPK KKEEKEERRG 550
    SSTVFLEDLS NIKHEVAKCC KPIPGDEILG VITRTKGLVL HEKSCSNLKN 600
    VLRLNPEKVK EVQLQASGYF QTDIRVVASD RIGLLSDITK VISESGSNIV 650
    SSMTNTREGK AVMDFTVEVK NKEHLEKIMK KIKSVEGVKI CKRLYH 696
    Length:696
    Mass (Da):80,570
    Last modified:August 1, 1998 - v1
    Checksum:i825F3FE6A556FF15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06975.1.
    PIRiA70373.
    RefSeqiNP_213573.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC06975; AAC06975; aq_844.
    GeneIDi1193554.
    KEGGiaae:aq_844.
    PATRICi20959066. VBIAquAeo85532_0658.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06975.1 .
    PIRi A70373.
    RefSeqi NP_213573.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67012.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_844.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC06975 ; AAC06975 ; aq_844 .
    GeneIDi 1193554.
    KEGGi aae:aq_844.
    PATRICi 20959066. VBIAquAeo85532_0658.

    Phylogenomic databases

    eggNOGi COG0317.
    HOGENOMi HOG000018299.
    KOi K01139.
    OMAi IGYITRG.
    OrthoDBi EOG6SV551.

    Enzyme and pathway databases

    UniPathwayi UPA00908 ; UER00886 .
    BioCyci AAEO224324:GJBH-603-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF01966. HD. 1 hit.
    PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiSPOT_AQUAE
    AccessioniPrimary (citable) accession number: O67012
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3