Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O67012 (SPOT_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

EC=3.1.7.2
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name=(ppGpp)ase
Gene names
Name:spoT
Ordered Locus Names:aq_844
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length696 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp By similarity.

Catalytic activity

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactor

Manganese By similarity.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 696696Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
PRO_0000166567

Regions

Domain49 – 14597HD
Domain623 – 69674ACT

Sequences

Sequence LengthMass (Da)Tools
O67012 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 825F3FE6A556FF15

FASTA69680,570
        10         20         30         40         50         60 
MSKLGEVSLE EDLEKLLSHY PQHAEEIQRA YEFAKEKHGE QKRKTGEPYI IHPLNVALKL 

        70         80         90        100        110        120 
AELGMDHETI IAALLHDTLE DTDTTYEEIK ERFGERVAKL VEGVTKIGKI KYKSEQAENY 

       130        140        150        160        170        180 
RKLILATAED PRVILLKLSD RLDNVKTLWV FREEKRKKIA KETMEIYAPL AHRLGVWSIK 

       190        200        210        220        230        240 
NELEDWAFKY LYPEEYEKVR NFVKESRKNL EEYLRKYVIP KVRKELEKYG IEAEIKYRSK 

       250        260        270        280        290        300 
HYYSIWEKTR RKGIRLEDVH DILGVRIIVN TVPECYTVLG IIHSLFRPVP GKFKDYISLP 

       310        320        330        340        350        360 
KPNLYQSLHT TVIADKGKLV EFQIRTWEMH ERAEKGIASH WAYKEGKNPS DAGVYSWLRE 

       370        380        390        400        410        420 
LVESIQGSTN PSEVLENLKS NLFFEEVFVF TPKGDLVVLP KGSTPVDLAY KIHTEVGNHC 

       430        440        450        460        470        480 
AGAKSNGRIV PLNYELKSGD VVEIITNPNK SPSYEWLSFV KTSRARNKIK QFLKKQERER 

       490        500        510        520        530        540 
YLSEGKRILE RIREKLGLSH EDLINKIRER VRFDTEEELL LALGKRKISS ANLIKLIFPK 

       550        560        570        580        590        600 
KKEEKEERRG SSTVFLEDLS NIKHEVAKCC KPIPGDEILG VITRTKGLVL HEKSCSNLKN 

       610        620        630        640        650        660 
VLRLNPEKVK EVQLQASGYF QTDIRVVASD RIGLLSDITK VISESGSNIV SSMTNTREGK 

       670        680        690 
AVMDFTVEVK NKEHLEKIMK KIKSVEGVKI CKRLYH 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC06975.1.
PIRA70373.
RefSeqNP_213573.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67012.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_844.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC06975; AAC06975; aq_844.
GeneID1193554.
KEGGaae:aq_844.
PATRIC20959066. VBIAquAeo85532_0658.

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018299.
KOK01139.
OMALDWLNFV.
OrthoDBEOG6SV551.
ProtClustDBCLSK2299520.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-603-MONOMER.
UniPathwayUPA00908; UER00886.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPOT_AQUAE
AccessionPrimary (citable) accession number: O67012
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 13, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways