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Protein

tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferase

Gene

trm1

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Dimethylates the guanine residues at position 26 and 27 of one or more tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.UniRule annotation1 Publication

Catalytic activityi

4 S-adenosyl-L-methionine + guanine(26)/guanine(27) in tRNA = 4 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26)/N(2)-dimethylguanine(27) in tRNA.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16631.
BRENDAi2.1.1.215. 396.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferaseUniRule annotation (EC:2.1.1.215UniRule annotation)
Gene namesi
Name:trm1UniRule annotation
Ordered Locus Names:aq_841
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476781 – 392tRNA (guanine(26)-N(2)/guanine(27)-N(2))-dimethyltransferaseAdd BLAST392

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_841.

Structurei

Secondary structure

1392
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Beta strandi9 – 13Combined sources5
Helixi30 – 32Combined sources3
Helixi33 – 50Combined sources18
Beta strandi54 – 60Combined sources7
Helixi65 – 73Combined sources9
Beta strandi77 – 83Combined sources7
Helixi87 – 99Combined sources13
Helixi104 – 106Combined sources3
Beta strandi107 – 110Combined sources4
Helixi114 – 119Combined sources6
Beta strandi126 – 131Combined sources6
Helixi138 – 140Combined sources3
Helixi141 – 147Combined sources7
Beta strandi148 – 158Combined sources11
Helixi161 – 164Combined sources4
Helixi169 – 176Combined sources8
Beta strandi177 – 179Combined sources3
Helixi186 – 203Combined sources18
Turni204 – 206Combined sources3
Beta strandi207 – 217Combined sources11
Beta strandi220 – 229Combined sources10
Helixi231 – 238Combined sources8
Beta strandi241 – 246Combined sources6
Turni248 – 250Combined sources3
Beta strandi253 – 256Combined sources4
Helixi259 – 261Combined sources3
Turni267 – 269Combined sources3
Beta strandi274 – 280Combined sources7
Helixi287 – 298Combined sources12
Helixi305 – 318Combined sources14
Helixi328 – 335Combined sources8
Helixi343 – 349Combined sources7
Beta strandi356 – 358Combined sources3
Beta strandi361 – 363Combined sources3
Helixi368 – 384Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AXSX-ray2.16A1-392[»]
3AXTX-ray2.49A1-392[»]
ProteinModelPortaliO67010.
SMRiO67010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 375Trm1 methyltransferaseUniRule annotationAdd BLAST374

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.UniRule annotation
Contains 1 Trm1 methyltransferase domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108K3F. Bacteria.
COG1867. LUCA.
HOGENOMiHOG000229931.
InParanoidiO67010.
KOiK00555.
OMAiFYNPRMA.
OrthoDBiPOG091H15VU.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00290. tRNA_dimethyltr_TRM1. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
IPR022923. TRM1_arc_bac.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIVQEGIAK IIVPEIPKTV SSDMPVFYNP RMRVNRDLAV LGLEYLCKKL
60 70 80 90 100
GRPVKVADPL SASGIRAIRF LLETSCVEKA YANDISSKAI EIMKENFKLN
110 120 130 140 150
NIPEDRYEIH GMEANFFLRK EWGFGFDYVD LDPFGTPVPF IESVALSMKR
160 170 180 190 200
GGILSLTATD TAPLSGTYPK TCMRRYMARP LRNEFKHEVG IRILIKKVIE
210 220 230 240 250
LAAQYDIAMI PIFAYSHLHY FKLFFVKERG VEKVDKLIEQ FGYIQYCFNC
260 270 280 290 300
MNREVVTDLY KFKEKCPHCG SKFHIGGPLW IGKLWDEEFT NFLYEEAQKR
310 320 330 340 350
EEIEKETKRI LKLIKEESQL QTVGFYVLSK LAEKVKLPAQ PPIRIAVKFF
360 370 380 390
NGVRTHFVGD GFRTNLSFEE VMKKMEELKE KQKEFLEKKK QG
Length:392
Mass (Da):45,479
Last modified:August 1, 1998 - v1
Checksum:iD2441A3754566043
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06976.1.
PIRiG70372.
RefSeqiNP_213571.1. NC_000918.1.
WP_010880509.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06976; AAC06976; aq_841.
GeneIDi1193460.
KEGGiaae:aq_841.
PATRICi20959062. VBIAquAeo85532_0656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06976.1.
PIRiG70372.
RefSeqiNP_213571.1. NC_000918.1.
WP_010880509.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AXSX-ray2.16A1-392[»]
3AXTX-ray2.49A1-392[»]
ProteinModelPortaliO67010.
SMRiO67010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06976; AAC06976; aq_841.
GeneIDi1193460.
KEGGiaae:aq_841.
PATRICi20959062. VBIAquAeo85532_0656.

Phylogenomic databases

eggNOGiENOG4108K3F. Bacteria.
COG1867. LUCA.
HOGENOMiHOG000229931.
InParanoidiO67010.
KOiK00555.
OMAiFYNPRMA.
OrthoDBiPOG091H15VU.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16631.
BRENDAi2.1.1.215. 396.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00290. tRNA_dimethyltr_TRM1. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
IPR022923. TRM1_arc_bac.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM1_AQUAE
AccessioniPrimary (citable) accession number: O67010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.