O67009 (ILVD_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	aq_837

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103426
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	193	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O67009 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9AE9D2E7DC1F9B1A	FASTA	555	59,547
	10 20 30 40 50 60 MKFRSDKVKK GIERAPHRAL LRACGLSDED FDKPLIGIAN SYIDIIPGHV HLREFVEPIK 70 80 90 100 110 120 EEVRKLGGVP IEFNVIGVDD GIAMGHEGMH YSLPSRELIA DSIETVVNAH QLDALICIPN 130 140 150 160 170 180 CDKIVPGMLM GALRVNVPTV FISGGPMLAG EVNGQKVDLI SVFEGIGKVK RGEISEQELK 190 200 210 220 230 240 VIEASACPTC GSCSGMFTAN SMNCLTEVLG LALPGNGTIL AIDPRREILA RNAVKALFEL 250 260 270 280 290 300 LEKDVKPRDI VTEEALDDAF TVDIAMGGSS NTILHLLAIA REAGIEYNLA KINEISKRTP 310 320 330 340 350 360 TICKISPASH YHIEDLDRVG GIPTIMKELS KLGLLHTERK TVSGKTIGEI ISDAPDADGE 370 380 390 400 410 420 VVRTIENPYS KDGGIAILFG NLAPEGAVVK TAGVDPKMLT FKGKAICFDS EEEAIEGILG 430 440 450 460 470 480 GKVKPGHVVV IRYEGPKGGP GMREMLSPTS AIMGMGLGDK VALITDGRFS GGTRGACVGH 490 500 510 520 530 540 ISPEAAAGGP IGIVKDGDEI LIDIPNRRIE LLISEEEFNE RMKNFKPKQK EIKSSWLRRY 550 AKLVTSASKG AILEA

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06971.1.
PIR	F70372.
RefSeq	NP_213570.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67009.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193459.
GenomeReviews	Gene locus aq_837 in contig AE000657_GR.
KEGG	aae:aq_837.
NMPDR	fig|224324.1.peg.585.
PATRIC	20959060. VBIAquAeo85532_0655.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	O67009.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_837-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AQUAE

Accession

Primary (citable) accession number: O67009

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program