Reviewed, UniProtKB/Swiss-Prot O67004 (SURE_AQUAE)

Names and origin

Protein names

Recommended name:
    5'-nucleotidase surE
    EC=3.1.3.5
Alternative name(s):
    Nucleoside 5'-monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	aq_832

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the surE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 251

251

5'-nucleotidase surE HAMAP MF_00060

PRO_0000111788

Sites

Metal binding

9

1

Divalent metal cation By similarity

Metal binding

10

1

Divalent metal cation By similarity

Metal binding

40

1

Divalent metal cation By similarity

Metal binding

94

1

Divalent metal cation By similarity

Secondary structure

1

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251

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Helix

15 – 24

10

Turn

25 – 27

3

Beta strand

28 – 37

10

Beta strand

52 – 58

7

Beta strand

61 – 64

4

Helix

69 – 78

10

Turn

79 – 83

5

Beta strand

87 – 96

10

Helix

100 – 105

6

Helix

107 – 117

11

Beta strand

122 – 128

7

Beta strand

130 – 132

3

Helix

135 – 152

18

Beta strand

158 – 167

10

Helix

169 – 171

3

Beta strand

174 – 177

4

Beta strand

184 – 193

10

Beta strand

199 – 207

9

Turn

208 – 211

4

Helix

217 – 222

6

Beta strand

225 – 233

9

Helix

238 – 248

11

Sequences

	Sequence		Length	Mass (Da)
	O67004-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7D36DDD8BEB1C55B	FASTA	251	28,165
	10 20 30 40 50 60 MPTFLLVNDD GYFSPGINAL REALKSLGRV VVVAPDRNLS GVGHSLTFTE PLKMRKIDTD 70 80 90 100 110 120 FYTVIDGTPA DCVHLGYRVI LEEKKPDLVL SGINEGPNLG EDITYSGTVS GAMEGRILGI 130 140 150 160 170 180 PSIAFSAFGR ENIMFEEIAK VCVDIVKKVL NEGIPEDTYL NVNIPNLRYE EIKGIKVTRQ 190 200 210 220 230 240 GKRAYKERVF KYIDPYGKPF YWIAAEEFGW HAEEGTDYWA VLNGYVSVTP LHLDLTNYKV 250 MKSIKYLEDS P

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000657 Genomic DNA. Translation: AAC06967.1.

PIR

A70372.

RefSeq

NP_213565.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PHJ	X-ray	1.50	A/B	1-251	[»]

ModBase

Search...

Genome annotation databases

GeneID

1193454.

GenomeReviews

Gene locus aq_832 in contig AE000657_GR.

KEGG

aae:aq_832.

NMPDR

fig|224324.1.peg.580.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O67004.

OMA

NLNIPPC.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_832-MON.

BRENDA

3.1.3.5. 189781.

Family and domain databases

HAMAP

MF_00060.
[Tree]

InterPro

IPR002828. SurE-like_Pase/nucleotidase.
[Graphical view]

Gene3D

G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.

Pfam

PF01975. SurE. 1 hit.
[Graphical view]

ProDom

PD005378. SurE. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00087. surE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

SURE_AQUAE

Accession

Primary (citable) accession number: O67004

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)