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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111IronUniRule annotation
Metal bindingi115 – 1151IronUniRule annotation
Binding sitei166 – 1661SubstrateUniRule annotation
Binding sitei179 – 1791Substrate; via amide nitrogenUniRule annotation
Binding sitei183 – 1831SubstrateUniRule annotation
Binding sitei268 – 2681SubstrateUniRule annotation
Metal bindingi296 – 2961IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-571-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:aq_801
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096956Add
BLAST

Proteomic databases

PRIDEiO66986.

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_801.

Structurei

3D structure databases

ProteinModelPortaliO66986.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1375Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
InParanoidiO66986.
KOiK01409.
OMAiYTKEPGL.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLAVETSC DETALAIYDD QKGVLGNVIL SQAVVHSPFG GVVPELSARE
60 70 80 90 100
HTRNILPIFD RLLKESRINL EEIDFISFTL TPGLILSLVV GVAFAKALAY
110 120 130 140 150
EYRKPLVPVH HLEGHIYSVF LEKKVEYPFL ALIISGGHTD LYLVRDFGRY
160 170 180 190 200
DFLGGTLDDA VGEAYDKVAK MLGLGYPGGP IIDRLAKEGK KLYPLPKPLM
210 220 230 240 250
EEGNLNFSFS GLKTAILNLL KKEKNVRKED IAYSFQETVV EILLEKSLWA
260 270 280 290 300
MKKTGIKRLV VVGGVSANSR LREVFKKASQ EYGFELYIPH PSLSTDNALM
310 320 330
IAYAGMERFK RGVVAPLDVN PQPNIPLEEF GRIWT
Length:335
Mass (Da):37,370
Last modified:August 1, 1998 - v1
Checksum:iD3B1606646908EC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06951.1.
PIRiG70369.
RefSeqiNP_213547.1. NC_000918.1.
WP_010880485.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06951; AAC06951; aq_801.
GeneIDi1193436.
KEGGiaae:aq_801.
PATRICi20959002. VBIAquAeo85532_0632.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06951.1.
PIRiG70369.
RefSeqiNP_213547.1. NC_000918.1.
WP_010880485.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66986.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_801.

Proteomic databases

PRIDEiO66986.

Protocols and materials databases

DNASUi1193436.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06951; AAC06951; aq_801.
GeneIDi1193436.
KEGGiaae:aq_801.
PATRICi20959002. VBIAquAeo85532_0632.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
InParanoidiO66986.
KOiK01409.
OMAiYTKEPGL.
OrthoDBiEOG6K402S.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-571-MONOMER.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiTSAD_AQUAE
AccessioniPrimary (citable) accession number: O66986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.