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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111IronUniRule annotation1
Metal bindingi115IronUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Binding sitei179Substrate; via amide nitrogenUniRule annotation1
Binding sitei183SubstrateUniRule annotation1
Binding sitei268SubstrateUniRule annotation1
Metal bindingi296IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-567-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:aq_801
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969561 – 335tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST335

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_801

Structurei

3D structure databases

ProteinModelPortaliO66986
SMRiO66986
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 137Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM Bacteria
COG0533 LUCA
HOGENOMiHOG000109568
InParanoidiO66986
KOiK01409
OMAiHLEGHIY
OrthoDBiPOG091H010B

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR017860 Peptidase_M22_CS
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

O66986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLAVETSC DETALAIYDD QKGVLGNVIL SQAVVHSPFG GVVPELSARE
60 70 80 90 100
HTRNILPIFD RLLKESRINL EEIDFISFTL TPGLILSLVV GVAFAKALAY
110 120 130 140 150
EYRKPLVPVH HLEGHIYSVF LEKKVEYPFL ALIISGGHTD LYLVRDFGRY
160 170 180 190 200
DFLGGTLDDA VGEAYDKVAK MLGLGYPGGP IIDRLAKEGK KLYPLPKPLM
210 220 230 240 250
EEGNLNFSFS GLKTAILNLL KKEKNVRKED IAYSFQETVV EILLEKSLWA
260 270 280 290 300
MKKTGIKRLV VVGGVSANSR LREVFKKASQ EYGFELYIPH PSLSTDNALM
310 320 330
IAYAGMERFK RGVVAPLDVN PQPNIPLEEF GRIWT
Length:335
Mass (Da):37,370
Last modified:August 1, 1998 - v1
Checksum:iD3B1606646908EC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06951.1
PIRiG70369
RefSeqiNP_213547.1, NC_000918.1
WP_010880485.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC06951; AAC06951; aq_801
GeneIDi1193436
KEGGiaae:aq_801
PATRICifig|224324.8.peg.632

Similar proteinsi

Entry informationi

Entry nameiTSAD_AQUAE
AccessioniPrimary (citable) accession number: O66986
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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