Reviewed, UniProtKB/Swiss-Prot O66973 (NADB_AQUAE)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	aq_777

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	L-aspartate oxidase		PRO_0000184378
Regions
	Nucleotide binding	20 – 34	15	FAD Potential
Sites
	Active site	234	1	By similarity
	Active site	250	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66973-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B6D5AD83438D0C42	FASTA	510	57,793
	10 20 30 40 50 60 MNYFLRFDTS LLPEEEAKVL ICGSGIGGLA TAISLKELGI EPLILTRGIG NTYYSQGGIA 70 80 90 100 110 120 AAVLPDDSPY LHYCDTLRAG RYLNHELHTK LLTYEGIFRI LDLERWGVDF DKKNGFYETT 130 140 150 160 170 180 LEGGHSKPRV LKVKDYTGRE IYTKLLKKTE ELGIKILHGE LQEIIIEENG VQGVIYSEEG 190 200 210 220 230 240 SLKFIRTPLL ILATGGAASM YYYTSNPKKV RGDAIGIAFR MGATVKNPEF VQFHPTVLKG 250 260 270 280 290 300 TNLLISEAVR GEGAILVNDR GERFVNELLP RDEVARAIYN QIKRGREVYL DLRPVAQKGI 310 320 330 340 350 360 KIEERFPTIY SFLRERGYDP YREPVPIIPA AHYYIGGIEV DDRGRTSIQG LYAVGECSCT 370 380 390 400 410 420 GIHGANRLAS NSLLEGVVFG HRTAYQVYLD LKILKPSKVN FRSERAGSDK PNLGIEELKK 430 440 450 460 470 480 LMWNYVGLER DEEGLTYVKK RILSVLSQAK HWEPTPQNRQ LYDILLVALC TVEGALMRKE 490 500 510 SRGVHYRKDY PTEREVYRRD TIITRDLFEL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06932.1.
PIR	B70368.
RefSeq	NP_213534.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1193423.
GenomeReviews	Gene locus aq_777 in contig AE000657_GR.
KEGG	aae:aq_777.
NMPDR	fig|224324.1.peg.549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66973.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_777-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR005288. NadB. IPR001100. Pyr_nuc-diS_OxRdtase. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_AQUAE

Accession

Primary (citable) accession number: O66973

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)