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Protein

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Gene

mnmG

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126FAD; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei181FAD1 Publication1
Binding sitei377FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19FAD1 Publication6
Nucleotide bindingi280 – 294NADSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene namesi
Name:mnmG
Synonyms:gidA
Ordered Locus Names:aq_761
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170461 – 617tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGAdd BLAST617

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-48311N.
STRINGi224324.aq_761.

Structurei

Secondary structure

1617
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi9 – 13Combined sources5
Helixi17 – 28Combined sources12
Beta strandi33 – 38Combined sources6
Helixi40 – 42Combined sources3
Beta strandi50 – 53Combined sources4
Helixi57 – 67Combined sources11
Helixi71 – 78Combined sources8
Beta strandi79 – 86Combined sources8
Helixi91 – 93Combined sources3
Beta strandi95 – 100Combined sources6
Helixi102 – 114Combined sources13
Beta strandi119 – 124Combined sources6
Beta strandi126 – 141Combined sources16
Beta strandi146 – 148Combined sources3
Beta strandi150 – 154Combined sources5
Beta strandi163 – 166Combined sources4
Beta strandi169 – 172Combined sources4
Helixi184 – 190Combined sources7
Beta strandi196 – 202Combined sources7
Beta strandi205 – 207Combined sources3
Helixi208 – 210Combined sources3
Beta strandi216 – 219Combined sources4
Beta strandi230 – 234Combined sources5
Beta strandi249 – 253Combined sources5
Helixi256 – 264Combined sources9
Helixi265 – 268Combined sources4
Turni281 – 283Combined sources3
Helixi287 – 293Combined sources7
Beta strandi301 – 306Combined sources6
Beta strandi313 – 317Combined sources5
Helixi325 – 332Combined sources8
Beta strandi343 – 345Combined sources3
Beta strandi348 – 354Combined sources7
Helixi357 – 359Combined sources3
Beta strandi364 – 370Combined sources7
Beta strandi372 – 374Combined sources3
Helixi376 – 379Combined sources4
Helixi384 – 402Combined sources19
Turni412 – 414Combined sources3
Helixi416 – 427Combined sources12
Helixi435 – 437Combined sources3
Turni441 – 445Combined sources5
Helixi451 – 461Combined sources11
Beta strandi463 – 465Combined sources3
Helixi467 – 487Combined sources21
Beta strandi491 – 496Combined sources6
Beta strandi499 – 504Combined sources6
Helixi505 – 508Combined sources4
Turni509 – 512Combined sources4
Helixi515 – 522Combined sources8
Helixi530 – 552Combined sources23
Helixi554 – 560Combined sources7
Helixi570 – 572Combined sources3
Helixi578 – 587Combined sources10
Helixi592 – 595Combined sources4
Helixi603 – 612Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZXHX-ray3.20A/B1-617[»]
2ZXIX-ray2.30A/B/C/D1-617[»]
ProteinModelPortaliO66962.
SMRiO66962.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66962.

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.Curated

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201059.
InParanoidiO66962.
KOiK03495.
OMAiFRPGYAI.
OrthoDBiPOG091H01O0.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWVVDEFDV VVIGGGHAGI EAALAAARMG AKTAMFVLNA DTIGQMSCNP
60 70 80 90 100
AIGGIAKGIV VREIDALGGE MGKAIDQTGI QFKMLNTRKG KAVQSPRAQA
110 120 130 140 150
DKKRYREYMK KVCENQENLY IKQEEVVDII VKNNQVVGVR TNLGVEYKTK
160 170 180 190 200
AVVVTTGTFL NGVIYIGDKM IPGGRLGEPR SEGLSDFYRR FDFPLIRFKT
210 220 230 240 250
GTPARLDKRT IDFSALEVAP GDDPPPKFSF WTEPVGSYWF PKGKEQVNCW
260 270 280 290 300
ITYTTPKTHE IIRKNLHRTA LYGGLIKGIG PRYCPSIEDK IVKFPDKERH
310 320 330 340 350
QIFLEPEGLD TIEIYPNGLS TSLPEEVQWE MYRSIPGLEN VVLIRPAYAI
360 370 380 390 400
EYDVVPPTEL YPTLETKKIR GLFHAGNFNG TTGYEEAAGQ GIVAGINAAL
410 420 430 440 450
RAFGKEPIYL RRDESYIGVM IDDLTTKGVT EPYRLFTSRS EYRLYIRQDN
460 470 480 490 500
AILRLAKLGR ELGLLSEEQY KLVKELEREI EKWKEFYKSE RVSVAVGGDT
510 520 530 540 550
RSYSVATLMT MNYTLDDVKE KFGYEVPQHP YVKEEVEIQL KYEPYIERER
560 570 580 590 600
KLNEKLKKLE DTKIPPDIDY DKIPGLTKEA REKLKKFKPI TVGQASRIDG
610
ITPAAITALL VYLGKLD
Length:617
Mass (Da):69,644
Last modified:August 1, 1998 - v1
Checksum:i1DAB1BDFEFCBA6DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06917.1.
PIRiG70366.
RefSeqiNP_213523.1. NC_000918.1.
WP_010880461.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06917; AAC06917; aq_761.
GeneIDi1193412.
KEGGiaae:aq_761.
PATRICi20958950. VBIAquAeo85532_0606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06917.1.
PIRiG70366.
RefSeqiNP_213523.1. NC_000918.1.
WP_010880461.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZXHX-ray3.20A/B1-617[»]
2ZXIX-ray2.30A/B/C/D1-617[»]
ProteinModelPortaliO66962.
SMRiO66962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48311N.
STRINGi224324.aq_761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06917; AAC06917; aq_761.
GeneIDi1193412.
KEGGiaae:aq_761.
PATRICi20958950. VBIAquAeo85532_0606.

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201059.
InParanoidiO66962.
KOiK03495.
OMAiFRPGYAI.
OrthoDBiPOG091H01O0.

Miscellaneous databases

EvolutionaryTraceiO66962.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNMG_AQUAE
AccessioniPrimary (citable) accession number: O66962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.