Reviewed, UniProtKB/Swiss-Prot O66954 (PGMI_AQUAE)

Names and origin

Protein names

Recommended name:
    Bifunctional phosphoglucose/phosphomannose isomerase
Alternative name(s):
    Glucose-6-phosphate isomerase
      Short name=GPI
    EC=5.3.1.9
    Phosphoglucose isomerase
      Short name=PGI
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
    Phosphomannose isomerase
      Short name=PMI

Gene names

Ordered Locus Names:

aq_750

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency By similarity.
Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. D-mannose 6-phosphate = D-fructose 6-phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the PGI/PMI family. Contains 1 SIS domain.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Bifunctional phosphoglucose/phosphomannose isomerase		PRO_0000227793
Regions
	Domain	20 – 153	134	SIS
Sites
	Active site	204	1	Proton acceptor By similarity
	Active site	220	1	Proton donor By similarity
	Active site	313	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66954-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 732BD143902F5AC7	FASTA	320	36,847
	10 20 30 40 50 60 MEEVYKVVEG FYNQFEWEDI AKDLTPYKGI VFCGMGGSGI IGSFASKWLE HRSFNKPTFV 70 80 90 100 110 120 VKDYTLPPFV DRDYLVFCIS YSGNTEETLS NFEEAIGRGI KPLCITSNGK LMERAKEEGC 130 140 150 160 170 180 EIYEVPKGFQ PRYSLGFMLS KVLNLLGVDK DELEDAKENL KENLESLKQK GKEIANRIYG 190 200 210 220 230 240 YIPVVYSTPL TAHIAERWKG QINENSKSPA YFTILPEMHH NEVMGWSNPE LRNKFVYLLM 250 260 270 280 290 300 FDEKDHHRVK LRVDITKKIL EDFGVVPIML KGEGNSYLAR SLYLVHLADW VSVFLAELYG 310 320 YDPVPVKTIE RIKEELKKHA

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06906.1.
PIR	G70365.
RefSeq	NP_213515.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1193404.
GenomeReviews	Gene locus aq_750 in contig AE000657_GR.
KEGG	aae:aq_750.
NMPDR	fig|224324.1.peg.530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507538.
OMA	GNTEETL.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_750-MONOMER.
BRENDA	5.3.1.8. 189781. 5.3.1.9. 189781.
Family and domain databases
InterPro	IPR011857. Glu6P/Mann6P_isomerase. IPR019490. Glu6P/Mann6P_isomerase_C. IPR001347. SIS. [Graphical view]
Pfam	PF10432. bact-PGI_C. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
TIGRFAMs	TIGR02128. G6PI_arch. 1 hit.
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGMI_AQUAE

Accession

Primary (citable) accession number: O66954

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)