O66954 (PGMI_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional phosphoglucose/phosphomannose isomerase

Alternative name(s):
Glucose-6-phosphate isomerase
Short name=GPI
EC=5.3.1.9
Mannose-6-phosphate isomerase
EC=5.3.1.8
Phosphoglucose isomerase
Short name=PGI
Phosphomannose isomerase
Short name=PMI

Gene names

Ordered Locus Names:

aq_750

Organism

Aquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Aquifex › Aquifex aeolicus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency By similarity.
Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. D-mannose 6-phosphate = D-fructose 6-phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the PGI/PMI family. Contains 1 SIS domain.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Bifunctional phosphoglucose/phosphomannose isomerase		PRO_0000227793
Regions
	Domain	20 – 153	134	SIS
Sites
	Active site	204	1	Proton acceptor By similarity
	Active site	220	1	Proton donor By similarity
	Active site	313	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66954 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 732BD143902F5AC7	FASTA	320	36,847
	10 20 30 40 50 60 MEEVYKVVEG FYNQFEWEDI AKDLTPYKGI VFCGMGGSGI IGSFASKWLE HRSFNKPTFV 70 80 90 100 110 120 VKDYTLPPFV DRDYLVFCIS YSGNTEETLS NFEEAIGRGI KPLCITSNGK LMERAKEEGC 130 140 150 160 170 180 EIYEVPKGFQ PRYSLGFMLS KVLNLLGVDK DELEDAKENL KENLESLKQK GKEIANRIYG 190 200 210 220 230 240 YIPVVYSTPL TAHIAERWKG QINENSKSPA YFTILPEMHH NEVMGWSNPE LRNKFVYLLM 250 260 270 280 290 300 FDEKDHHRVK LRVDITKKIL EDFGVVPIML KGEGNSYLAR SLYLVHLADW VSVFLAELYG 310 320 YDPVPVKTIE RIKEELKKHA

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06906.1.
PIR	G70365.
RefSeq	NP_213515.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66954.
ModBase	Search...
Protein-protein interaction databases
STRING	224324.aq_750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC06906; AAC06906; aq_750.
GeneID	1193404.
KEGG	aae:aq_750.
PATRIC	20958934. VBIAquAeo85532_0598.
Phylogenomic databases
eggNOG	COG0166.
HOGENOM	HOG000020037.
KO	K15916.
OMA	PELNHNE.
ProtClustDB	PRK08674.
Enzyme and pathway databases
BioCyc	AAEO224324:GJBH-539-MONOMER.
Family and domain databases
InterPro	IPR011857. Glu6P/Mann6P_isomerase. IPR019490. Glu6P/Mann6P_isomerase_C. IPR001347. SIS. [Graphical view]
Pfam	PF10432. bact-PGI_C. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
TIGRFAMs	TIGR02128. G6PI_arch. 1 hit.
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGMI_AQUAE

Accession

Primary (citable) accession number: O66954

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program