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O66949

- PUR2_AQUAE

UniProt

O66949 - PUR2_AQUAE

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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Magnesium or manganeseUniRule annotation
Metal bindingi285 – 2851Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 19462ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-534-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:aq_742
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Phosphoribosylamine--glycine ligasePRO_0000151434Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_742.

Structurei

Secondary structure

1
424
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98Combined sources
Helixi10 – 1910Combined sources
Beta strandi26 – 327Combined sources
Helixi37 – 393Combined sources
Beta strandi41 – 444Combined sources
Helixi51 – 6111Combined sources
Beta strandi64 – 685Combined sources
Helixi71 – 755Combined sources
Helixi78 – 847Combined sources
Beta strandi89 – 913Combined sources
Turni94 – 974Combined sources
Helixi98 – 1014Combined sources
Helixi103 – 11210Combined sources
Beta strandi120 – 1245Combined sources
Helixi126 – 13611Combined sources
Beta strandi138 – 1469Combined sources
Beta strandi152 – 1587Combined sources
Helixi159 – 17012Combined sources
Helixi176 – 1794Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi190 – 20011Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi210 – 2123Combined sources
Beta strandi215 – 2173Combined sources
Turni218 – 2203Combined sources
Beta strandi221 – 2244Combined sources
Beta strandi228 – 2336Combined sources
Helixi239 – 24810Combined sources
Helixi250 – 26011Combined sources
Beta strandi265 – 27511Combined sources
Beta strandi278 – 28710Combined sources
Turni290 – 2923Combined sources
Helixi293 – 2986Combined sources
Helixi304 – 3129Combined sources
Beta strandi323 – 33210Combined sources
Turni334 – 3374Combined sources
Helixi349 – 3535Combined sources
Beta strandi358 – 36811Combined sources
Beta strandi371 – 3744Combined sources
Beta strandi376 – 38813Combined sources
Helixi389 – 40012Combined sources
Turni414 – 4185Combined sources
Helixi419 – 4213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YW2X-ray1.80A/B1-424[»]
2YYAX-ray2.40A/B1-424[»]
ProteinModelPortaliO66949.
SMRiO66949. Positions 1-423.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66949.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 313207ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
InParanoidiO66949.
KOiK01945.
OMAiYKGFLYA.
OrthoDBiEOG69SKD1.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66949-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLVVGNGG REHAIAWKVA QSPLVKELYV AKGNAGIWEI AKRVDISPTD
60 70 80 90 100
VEKLAEFAKN EGVDFTIVGP EAPLVEGIVD EFEKRGLKIF GPNKEAAKLE
110 120 130 140 150
GSKAFAKTFM KKYGIPTARY EVFTDFEKAK EYVEKVGAPI VVKADGLAAG
160 170 180 190 200
KGAVVCETVE KAIETLDRFL NKKIFGKSSE RVVIEEFLEG EEASYIVMIN
210 220 230 240 250
GDRYVPLPTS QDHKRLLDED KGPNTGGMGA YSPTPVINEE VEKRIREEIV
260 270 280 290 300
ERVIKGLKEE GIYYRGFLYA GLMITKEGPK VLEFNVRLGD PEAQPILMRV
310 320 330 340 350
KNDFLETLLN FYEGKDVHIK EDERYALDVV LASRGYPEKP ETGKIIHGLD
360 370 380 390 400
YLKSMEDVVV FHAGTKKEGN FTVTSGGRVL NVCAYGKTLK EAKERAYEAI
410 420
RYVCFEGMHY RKDIGDKAFK YLSE
Length:424
Mass (Da):47,430
Last modified:August 1, 1998 - v1
Checksum:i06EB10D23A68E2FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06907.1.
PIRiB70365.
RefSeqiNP_213510.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06907; AAC06907; aq_742.
GeneIDi1193399.
KEGGiaae:aq_742.
PATRICi20958924. VBIAquAeo85532_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06907.1 .
PIRi B70365.
RefSeqi NP_213510.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YW2 X-ray 1.80 A/B 1-424 [» ]
2YYA X-ray 2.40 A/B 1-424 [» ]
ProteinModelPortali O66949.
SMRi O66949. Positions 1-423.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_742.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06907 ; AAC06907 ; aq_742 .
GeneIDi 1193399.
KEGGi aae:aq_742.
PATRICi 20958924. VBIAquAeo85532_0593.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033463.
InParanoidi O66949.
KOi K01945.
OMAi YKGFLYA.
OrthoDBi EOG69SKD1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci AAEO224324:GJBH-534-MONOMER.

Miscellaneous databases

EvolutionaryTracei O66949.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPUR2_AQUAE
AccessioniPrimary (citable) accession number: O66949
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3