Reviewed, UniProtKB/Swiss-Prot O66936 (GLGB_AQUAE)

Names and originHide

Protein names

Recommended name:
1,4-alpha-glucan-branching enzyme
EC=2.4.1.18

Alternative name(s):
Glycogen-branching enzyme
Short name=BE
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	aq_722

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributesHide

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

OntologiesHide

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188673
Sites
	Active site	206	1	By similarity
	Active site	241	1	By similarity
	Active site	246	1	By similarity
	Active site	309	1	By similarity
	Active site	311	1	By similarity
	Active site	362	1	By similarity
	Active site	429	1	By similarity
	Active site	430	1	By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	O66936-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D65FCD8D49724125	FASTA	630	74,168
	10 20 30 40 50 60 MKKFSLISDY DVYLFKEGTH TRLYDKLGSH VIELNGKRYT FFAVWAPHAD YVSLIGDFNE 70 80 90 100 110 120 WDKGSTPMVK REDGSGIWEV LLEGDLTGSK YKYFIKNGNY EVDKSDPFAF FCEQPPGNAS 130 140 150 160 170 180 VVWKLNYRWN DSEYMKKRKR VNSHDSPISI YEVHVGSWRR VPEEGNRFLS YRELAEYLPY 190 200 210 220 230 240 YVKEMGFTHV EFLPVMEHPF YGSWGYQITG YFAPTSRYGT PQDFMYLIDK LHQEGIGVIL 250 260 270 280 290 300 DWVPSHFPTD AHGLAYFDGT HLYEYEDWRK RWHPDWNSFV FDYGKPEVRS FLLSSAHFWL 310 320 330 340 350 360 DKYHADGLRV DAVASMLYLD YSRKEWVPNI YGGKENLEAI EFLRKFNESV YRNFPDVQTI 370 380 390 400 410 420 AEESTAWPMV SRPTYVGGLG FGMKWNMGWM NDTLFYFSKD PIYRKYHHEV LTFSIWYAFS 430 440 450 460 470 480 ENFVLPLSHD EVVHGKGSLI GKMPGDYWQK FANLRALFGY MWAHPGKKLL FMGGEFGQFK 490 500 510 520 530 540 EWDHETSLDW HLLEYPSHRG IQRLVKDLNE VYRREKALHE TDFSPEGFEW VDFHDWEKSV 550 560 570 580 590 600 ISFLRKDKSG KEIILVVCNF TPVPRYDYRV GVPKGGYWRE IMNTDAKEYW GSGMGNLGGK 610 620 630 EADKIPWHGR KFSLSLTLPP LSVIYLKHEG

ReferencesHide

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06895.1.
PIR	D70363.
RefSeq	NP_213496.1.
3D structure databases
SMR	O66936. Positions 19-629.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1193385.
GenomeReviews	Gene locus aq_722 in contig AE000657_GR.
KEGG	aae:aq_722.
NMPDR	fig|224324.1.peg.511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RVYHQNG.
PhylomeDB	O66936.
ProtClustDB	PRK12313.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_722-MONOMER.
BRENDA	2.4.1.18. 189781.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry informationHide

Entry name

GLGB_AQUAE

Accession

Primary (citable) accession number: O66936

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	August 1, 1998
Last modified:	July 13, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)