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O66936 (GLGB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:aq_722
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6306301,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188673

Sites

Active site3111Nucleophile By similarity
Active site3621Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
O66936 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: D65FCD8D49724125

FASTA63074,168
        10         20         30         40         50         60 
MKKFSLISDY DVYLFKEGTH TRLYDKLGSH VIELNGKRYT FFAVWAPHAD YVSLIGDFNE 

        70         80         90        100        110        120 
WDKGSTPMVK REDGSGIWEV LLEGDLTGSK YKYFIKNGNY EVDKSDPFAF FCEQPPGNAS 

       130        140        150        160        170        180 
VVWKLNYRWN DSEYMKKRKR VNSHDSPISI YEVHVGSWRR VPEEGNRFLS YRELAEYLPY 

       190        200        210        220        230        240 
YVKEMGFTHV EFLPVMEHPF YGSWGYQITG YFAPTSRYGT PQDFMYLIDK LHQEGIGVIL 

       250        260        270        280        290        300 
DWVPSHFPTD AHGLAYFDGT HLYEYEDWRK RWHPDWNSFV FDYGKPEVRS FLLSSAHFWL 

       310        320        330        340        350        360 
DKYHADGLRV DAVASMLYLD YSRKEWVPNI YGGKENLEAI EFLRKFNESV YRNFPDVQTI 

       370        380        390        400        410        420 
AEESTAWPMV SRPTYVGGLG FGMKWNMGWM NDTLFYFSKD PIYRKYHHEV LTFSIWYAFS 

       430        440        450        460        470        480 
ENFVLPLSHD EVVHGKGSLI GKMPGDYWQK FANLRALFGY MWAHPGKKLL FMGGEFGQFK 

       490        500        510        520        530        540 
EWDHETSLDW HLLEYPSHRG IQRLVKDLNE VYRREKALHE TDFSPEGFEW VDFHDWEKSV 

       550        560        570        580        590        600 
ISFLRKDKSG KEIILVVCNF TPVPRYDYRV GVPKGGYWRE IMNTDAKEYW GSGMGNLGGK 

       610        620        630 
EADKIPWHGR KFSLSLTLPP LSVIYLKHEG 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC06895.1.
PIRD70363.
RefSeqNP_213496.1. NC_000918.1.

3D structure databases

ProteinModelPortalO66936.
SMRO66936. Positions 19-629.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_722.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC06895; AAC06895; aq_722.
GeneID1193385.
KEGGaae:aq_722.
PATRIC20958896. VBIAquAeo85532_0579.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-520-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_AQUAE
AccessionPrimary (citable) accession number: O66936
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries