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Protein

3-deoxy-manno-octulosonate cytidylyltransferase

Gene

kdsB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.UniRule annotation

Catalytic activityi

CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate.UniRule annotation

Pathwayi: CMP-3-deoxy-D-manno-octulosonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB)
This subpathway is part of the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP, the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BRENDAi2.7.7.38. 396.
UniPathwayiUPA00030.
UPA00358; UER00476.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferaseUniRule annotation (EC:2.7.7.38UniRule annotation)
Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthaseUniRule annotation
Short name:
CKSUniRule annotation
Short name:
CMP-KDO synthaseUniRule annotation
Gene namesi
Name:kdsBUniRule annotation
Ordered Locus Names:aq_692
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001884961 – 2343-deoxy-manno-octulosonate cytidylyltransferaseAdd BLAST234

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_692.

Structurei

Secondary structure

1234
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Turni14 – 18Combined sources5
Helixi19 – 21Combined sources3
Helixi29 – 38Combined sources10
Turni39 – 41Combined sources3
Beta strandi44 – 49Combined sources6
Helixi51 – 57Combined sources7
Turni58 – 60Combined sources3
Beta strandi61 – 65Combined sources5
Helixi73 – 81Combined sources9
Beta strandi87 – 91Combined sources5
Helixi101 – 113Combined sources13
Beta strandi116 – 123Combined sources8
Helixi126 – 129Combined sources4
Beta strandi135 – 138Combined sources4
Beta strandi142 – 150Combined sources9
Beta strandi164 – 173Combined sources10
Helixi174 – 182Combined sources9
Helixi187 – 192Combined sources6
Helixi196 – 201Combined sources6
Beta strandi207 – 210Combined sources4
Helixi221 – 230Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y6PX-ray2.10A/B/C1-234[»]
ProteinModelPortaliO66914.
SMRiO66914.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KdsB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CET. Bacteria.
COG1212. LUCA.
HOGENOMiHOG000007602.
InParanoidiO66914.
KOiK00979.
OMAiNSGTERC.
OrthoDBiPOG091H00UN.

Family and domain databases

CDDicd02517. CMP-KDO-Synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00057. KdsB. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR004528. KdsB.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR21485:SF4. PTHR21485:SF4. 1 hit.
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00466. kdsB. 1 hit.

Sequencei

Sequence statusi: Complete.

O66914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRAVIIPAR LGSTRLKEKP LKNLLGKPLI RWVVEGLVKT GERVILATDS
60 70 80 90 100
ERVKEVVEDL CEVFLTPSDL PSGSDRVLYV VRDLDVDLII NYQGDEPFVY
110 120 130 140 150
EEDIKLIFRE LEKGERVVTL ARKDKEAYER PEDVKVVLDR EGYALYFSRS
160 170 180 190 200
PIPYFRKNDT FYPLKHVGIY GFRKETLMEF GAMPPSKLEQ IEGLEQLRLL
210 220 230
ENGIKIKVLI TENYYHGVDT EEDLKIVEEK LKNL
Length:234
Mass (Da):27,173
Last modified:August 1, 1998 - v1
Checksum:iC838A2B7B84437C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06870.1.
PIRiF70360.
RefSeqiNP_213474.1. NC_000918.1.
WP_010880412.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06870; AAC06870; aq_692.
GeneIDi1193976.
KEGGiaae:aq_692.
PATRICi20958850. VBIAquAeo85532_0556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06870.1.
PIRiF70360.
RefSeqiNP_213474.1. NC_000918.1.
WP_010880412.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y6PX-ray2.10A/B/C1-234[»]
ProteinModelPortaliO66914.
SMRiO66914.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_692.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06870; AAC06870; aq_692.
GeneIDi1193976.
KEGGiaae:aq_692.
PATRICi20958850. VBIAquAeo85532_0556.

Phylogenomic databases

eggNOGiENOG4105CET. Bacteria.
COG1212. LUCA.
HOGENOMiHOG000007602.
InParanoidiO66914.
KOiK00979.
OMAiNSGTERC.
OrthoDBiPOG091H00UN.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00358; UER00476.
BRENDAi2.7.7.38. 396.

Family and domain databases

CDDicd02517. CMP-KDO-Synthetase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00057. KdsB. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR004528. KdsB.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR21485:SF4. PTHR21485:SF4. 1 hit.
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00466. kdsB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKDSB_AQUAE
AccessioniPrimary (citable) accession number: O66914
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.