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O66893

- TOP1_AQUAE

UniProt

O66893 - TOP1_AQUAE

Protein

DNA topoisomerase 1

Gene

topA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71Magnesium 1; catalyticUniRule annotation
    Sitei31 – 311Interaction with DNAUniRule annotation
    Metal bindingi79 – 791Magnesium 1; catalyticUniRule annotation
    Metal bindingi79 – 791Magnesium 2UniRule annotation
    Metal bindingi81 – 811Magnesium 2UniRule annotation
    Sitei136 – 1361Interaction with DNAUniRule annotation
    Sitei137 – 1371Interaction with DNAUniRule annotation
    Sitei140 – 1401Interaction with DNAUniRule annotation
    Sitei145 – 1451Interaction with DNAUniRule annotation
    Sitei152 – 1521Interaction with DNAUniRule annotation
    Active sitei281 – 2811O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei283 – 2831Interaction with DNAUniRule annotation
    Sitei467 – 4671Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-476-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:aq_657
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 540540DNA topoisomerase 1PRO_0000145138Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224324.aq_657.

    Structurei

    3D structure databases

    ProteinModelPortaliO66893.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 110110ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni161 – 1666Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0550.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiWINKAKK.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66893-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELFIVESPT KAKTIQKFLG KGFLVKATLG HVKDLPEKEL GVDLRTLKAK    50
    YVYKRGKKKL VEQLKKLSRR SSIVYLGTDP DREGEAIAYF LKKDLEKVNK 100
    NIKRAVFYEI TPEAIRESIR NAGDVNMNLV YAQFARRILD RLIGYLISPI 150
    LWKEFKNYKL SAGRVQSPAL RLIVEREREI QNFKVKKYYY VKALLRKGSE 200
    EFWAIYDYRY ENPSDAKIIA KKLEKGYFSV YKVEKKKEKV SPPKPFITSD 250
    LQSEANAKFG FSSERTQKLA QELYEKGYIT YPRTDSYRMN EKKAKEFMNY 300
    IEKKYGKEYV GRLRRFREKA TAQGAHECIR PTSLREEIPE REELRLLYDL 350
    IFRRTIASLM KEMLLEREKV TVEAITPELK HPVYLVAKGL KIVFDGWSRV 400
    YPSEITEEKL PELYEGDLLD LVKTTLEERK TQPPPRYTEG TLIKTLEKLG 450
    IGRPSTYATI VKTLKERGYV EVKKKALVPT EIAFQVVEFL MERFPTLMDY 500
    KFTAQMEEKL DLVEEGKLNW KSVVYEFMEK IFGKELEMVR 540
    Length:540
    Mass (Da):63,427
    Last modified:August 1, 1998 - v1
    Checksum:i89C0604DFF5B042E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06848.1.
    PIRiA70358.
    RefSeqiNP_213453.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC06848; AAC06848; aq_657.
    GeneIDi1193955.
    KEGGiaae:aq_657.
    PATRICi20958804. VBIAquAeo85532_0534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06848.1 .
    PIRi A70358.
    RefSeqi NP_213453.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O66893.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC06848 ; AAC06848 ; aq_657 .
    GeneIDi 1193955.
    KEGGi aae:aq_657.
    PATRICi 20958804. VBIAquAeo85532_0534.

    Phylogenomic databases

    eggNOGi COG0550.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi WINKAKK.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-476-MONOMER.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiTOP1_AQUAE
    AccessioniPrimary (citable) accession number: O66893
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3