Reviewed, UniProtKB/Swiss-Prot O66893 (TOP1_AQUAE)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	aq_657

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	DNA topoisomerase 1		PRO_0000145138
Sites
	Active site	281	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66893-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 89C0604DFF5B042E	FASTA	540	63,427
	10 20 30 40 50 60 MELFIVESPT KAKTIQKFLG KGFLVKATLG HVKDLPEKEL GVDLRTLKAK YVYKRGKKKL 70 80 90 100 110 120 VEQLKKLSRR SSIVYLGTDP DREGEAIAYF LKKDLEKVNK NIKRAVFYEI TPEAIRESIR 130 140 150 160 170 180 NAGDVNMNLV YAQFARRILD RLIGYLISPI LWKEFKNYKL SAGRVQSPAL RLIVEREREI 190 200 210 220 230 240 QNFKVKKYYY VKALLRKGSE EFWAIYDYRY ENPSDAKIIA KKLEKGYFSV YKVEKKKEKV 250 260 270 280 290 300 SPPKPFITSD LQSEANAKFG FSSERTQKLA QELYEKGYIT YPRTDSYRMN EKKAKEFMNY 310 320 330 340 350 360 IEKKYGKEYV GRLRRFREKA TAQGAHECIR PTSLREEIPE REELRLLYDL IFRRTIASLM 370 380 390 400 410 420 KEMLLEREKV TVEAITPELK HPVYLVAKGL KIVFDGWSRV YPSEITEEKL PELYEGDLLD 430 440 450 460 470 480 LVKTTLEERK TQPPPRYTEG TLIKTLEKLG IGRPSTYATI VKTLKERGYV EVKKKALVPT 490 500 510 520 530 540 EIAFQVVEFL MERFPTLMDY KFTAQMEEKL DLVEEGKLNW KSVVYEFMEK IFGKELEMVR

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06848.1.
PIR	A70358.
RefSeq	NP_213453.1.
3D structure databases
HSSP	HSSP built from PDB template 1GL9 based on UniProtKB O29238.
ModBase	Search...
Genome annotation databases
GeneID	1193955.
GenomeReviews	Gene locus aq_657 in contig AE000657_GR.
KEGG	aae:aq_657.
NMPDR	fig|224324.1.peg.468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66893.
OMA	MEAKLDD.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_657-MON.
BRENDA	5.99.1.2. 189781.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_AQUAE

Accession

Primary (citable) accession number: O66893

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)