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Protein

DNA topoisomerase 1

Gene

topA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi79Magnesium 1; catalyticUniRule annotation1
Metal bindingi79Magnesium 2UniRule annotation1
Metal bindingi81Magnesium 2UniRule annotation1
Active sitei281O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:aq_657
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451381 – 540DNA topoisomerase 1Add BLAST540

Proteomic databases

PRIDEiO66893.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31Interaction with DNAUniRule annotation1
Sitei136Interaction with DNAUniRule annotation1
Sitei137Interaction with DNAUniRule annotation1
Sitei140Interaction with DNAUniRule annotation1
Sitei145Interaction with DNAUniRule annotation1
Sitei152Interaction with DNAUniRule annotation1
Sitei283Interaction with DNAUniRule annotation1
Sitei467Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi224324.aq_657.

Structurei

3D structure databases

ProteinModelPortaliO66893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 110ToprimUniRule annotationAdd BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 166Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000004018.
InParanoidiO66893.
KOiK03168.
OMAiYNWVIDY.
OrthoDBiPOG091H02BN.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELFIVESPT KAKTIQKFLG KGFLVKATLG HVKDLPEKEL GVDLRTLKAK
60 70 80 90 100
YVYKRGKKKL VEQLKKLSRR SSIVYLGTDP DREGEAIAYF LKKDLEKVNK
110 120 130 140 150
NIKRAVFYEI TPEAIRESIR NAGDVNMNLV YAQFARRILD RLIGYLISPI
160 170 180 190 200
LWKEFKNYKL SAGRVQSPAL RLIVEREREI QNFKVKKYYY VKALLRKGSE
210 220 230 240 250
EFWAIYDYRY ENPSDAKIIA KKLEKGYFSV YKVEKKKEKV SPPKPFITSD
260 270 280 290 300
LQSEANAKFG FSSERTQKLA QELYEKGYIT YPRTDSYRMN EKKAKEFMNY
310 320 330 340 350
IEKKYGKEYV GRLRRFREKA TAQGAHECIR PTSLREEIPE REELRLLYDL
360 370 380 390 400
IFRRTIASLM KEMLLEREKV TVEAITPELK HPVYLVAKGL KIVFDGWSRV
410 420 430 440 450
YPSEITEEKL PELYEGDLLD LVKTTLEERK TQPPPRYTEG TLIKTLEKLG
460 470 480 490 500
IGRPSTYATI VKTLKERGYV EVKKKALVPT EIAFQVVEFL MERFPTLMDY
510 520 530 540
KFTAQMEEKL DLVEEGKLNW KSVVYEFMEK IFGKELEMVR
Length:540
Mass (Da):63,427
Last modified:August 1, 1998 - v1
Checksum:i89C0604DFF5B042E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06848.1.
PIRiA70358.
RefSeqiNP_213453.1. NC_000918.1.
WP_010880391.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06848; AAC06848; aq_657.
GeneIDi1193955.
KEGGiaae:aq_657.
PATRICi20958804. VBIAquAeo85532_0534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06848.1.
PIRiA70358.
RefSeqiNP_213453.1. NC_000918.1.
WP_010880391.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66893.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_657.

Proteomic databases

PRIDEiO66893.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06848; AAC06848; aq_657.
GeneIDi1193955.
KEGGiaae:aq_657.
PATRICi20958804. VBIAquAeo85532_0534.

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000004018.
InParanoidiO66893.
KOiK03168.
OMAiYNWVIDY.
OrthoDBiPOG091H02BN.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP1_AQUAE
AccessioniPrimary (citable) accession number: O66893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.