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O66893

- TOP1_AQUAE

UniProt

O66893 - TOP1_AQUAE

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Protein

DNA topoisomerase 1

Gene
topA, aq_657
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium 1; catalytic By similarity
Sitei31 – 311Interaction with DNA By similarity
Metal bindingi79 – 791Magnesium 1; catalytic By similarity
Metal bindingi79 – 791Magnesium 2 By similarity
Metal bindingi81 – 811Magnesium 2 By similarity
Sitei136 – 1361Interaction with DNA By similarity
Sitei137 – 1371Interaction with DNA By similarity
Sitei140 – 1401Interaction with DNA By similarity
Sitei145 – 1451Interaction with DNA By similarity
Sitei152 – 1521Interaction with DNA By similarity
Active sitei281 – 2811O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei283 – 2831Interaction with DNA By similarity
Sitei467 – 4671Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-476-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:aq_657
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540DNA topoisomerase 1UniRule annotationPRO_0000145138Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_657.

Structurei

3D structure databases

ProteinModelPortaliO66893.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 110110ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1666Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000004018.
KOiK03168.
OMAiWINKAKK.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66893-1 [UniParc]FASTAAdd to Basket

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MELFIVESPT KAKTIQKFLG KGFLVKATLG HVKDLPEKEL GVDLRTLKAK    50
YVYKRGKKKL VEQLKKLSRR SSIVYLGTDP DREGEAIAYF LKKDLEKVNK 100
NIKRAVFYEI TPEAIRESIR NAGDVNMNLV YAQFARRILD RLIGYLISPI 150
LWKEFKNYKL SAGRVQSPAL RLIVEREREI QNFKVKKYYY VKALLRKGSE 200
EFWAIYDYRY ENPSDAKIIA KKLEKGYFSV YKVEKKKEKV SPPKPFITSD 250
LQSEANAKFG FSSERTQKLA QELYEKGYIT YPRTDSYRMN EKKAKEFMNY 300
IEKKYGKEYV GRLRRFREKA TAQGAHECIR PTSLREEIPE REELRLLYDL 350
IFRRTIASLM KEMLLEREKV TVEAITPELK HPVYLVAKGL KIVFDGWSRV 400
YPSEITEEKL PELYEGDLLD LVKTTLEERK TQPPPRYTEG TLIKTLEKLG 450
IGRPSTYATI VKTLKERGYV EVKKKALVPT EIAFQVVEFL MERFPTLMDY 500
KFTAQMEEKL DLVEEGKLNW KSVVYEFMEK IFGKELEMVR 540
Length:540
Mass (Da):63,427
Last modified:August 1, 1998 - v1
Checksum:i89C0604DFF5B042E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06848.1.
PIRiA70358.
RefSeqiNP_213453.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06848; AAC06848; aq_657.
GeneIDi1193955.
KEGGiaae:aq_657.
PATRICi20958804. VBIAquAeo85532_0534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06848.1 .
PIRi A70358.
RefSeqi NP_213453.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66893.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06848 ; AAC06848 ; aq_657 .
GeneIDi 1193955.
KEGGi aae:aq_657.
PATRICi 20958804. VBIAquAeo85532_0534.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000004018.
KOi K03168.
OMAi WINKAKK.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-476-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiTOP1_AQUAE
AccessioniPrimary (citable) accession number: O66893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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