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O66893

- TOP1_AQUAE

UniProt

O66893 - TOP1_AQUAE

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg(2+) per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium 1; catalyticUniRule annotation
Sitei31 – 311Interaction with DNAUniRule annotation
Metal bindingi79 – 791Magnesium 1; catalyticUniRule annotation
Metal bindingi79 – 791Magnesium 2UniRule annotation
Metal bindingi81 – 811Magnesium 2UniRule annotation
Sitei136 – 1361Interaction with DNAUniRule annotation
Sitei137 – 1371Interaction with DNAUniRule annotation
Sitei140 – 1401Interaction with DNAUniRule annotation
Sitei145 – 1451Interaction with DNAUniRule annotation
Sitei152 – 1521Interaction with DNAUniRule annotation
Active sitei281 – 2811O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei283 – 2831Interaction with DNAUniRule annotation
Sitei467 – 4671Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-476-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:aq_657
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540DNA topoisomerase 1PRO_0000145138Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_657.

Structurei

3D structure databases

ProteinModelPortaliO66893.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 110110ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1666Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000004018.
InParanoidiO66893.
KOiK03168.
OMAiWINKAKK.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66893-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELFIVESPT KAKTIQKFLG KGFLVKATLG HVKDLPEKEL GVDLRTLKAK
60 70 80 90 100
YVYKRGKKKL VEQLKKLSRR SSIVYLGTDP DREGEAIAYF LKKDLEKVNK
110 120 130 140 150
NIKRAVFYEI TPEAIRESIR NAGDVNMNLV YAQFARRILD RLIGYLISPI
160 170 180 190 200
LWKEFKNYKL SAGRVQSPAL RLIVEREREI QNFKVKKYYY VKALLRKGSE
210 220 230 240 250
EFWAIYDYRY ENPSDAKIIA KKLEKGYFSV YKVEKKKEKV SPPKPFITSD
260 270 280 290 300
LQSEANAKFG FSSERTQKLA QELYEKGYIT YPRTDSYRMN EKKAKEFMNY
310 320 330 340 350
IEKKYGKEYV GRLRRFREKA TAQGAHECIR PTSLREEIPE REELRLLYDL
360 370 380 390 400
IFRRTIASLM KEMLLEREKV TVEAITPELK HPVYLVAKGL KIVFDGWSRV
410 420 430 440 450
YPSEITEEKL PELYEGDLLD LVKTTLEERK TQPPPRYTEG TLIKTLEKLG
460 470 480 490 500
IGRPSTYATI VKTLKERGYV EVKKKALVPT EIAFQVVEFL MERFPTLMDY
510 520 530 540
KFTAQMEEKL DLVEEGKLNW KSVVYEFMEK IFGKELEMVR
Length:540
Mass (Da):63,427
Last modified:August 1, 1998 - v1
Checksum:i89C0604DFF5B042E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06848.1.
PIRiA70358.
RefSeqiNP_213453.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06848; AAC06848; aq_657.
GeneIDi1193955.
KEGGiaae:aq_657.
PATRICi20958804. VBIAquAeo85532_0534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06848.1 .
PIRi A70358.
RefSeqi NP_213453.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66893.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06848 ; AAC06848 ; aq_657 .
GeneIDi 1193955.
KEGGi aae:aq_657.
PATRICi 20958804. VBIAquAeo85532_0534.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000004018.
InParanoidi O66893.
KOi K03168.
OMAi WINKAKK.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-476-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiTOP1_AQUAE
AccessioniPrimary (citable) accession number: O66893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3