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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103ATPBy similarity1
Active sitei329Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi305 – 309ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-463-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:aq_642
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564151 – 360RNA 3'-terminal phosphate cyclaseAdd BLAST360

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_642

Structurei

3D structure databases

ProteinModelPortaliO66884
SMRiO66884
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CKX Bacteria
COG0430 LUCA
HOGENOMiHOG000015264
InParanoidiO66884
KOiK01974
OMAiANRPKPG
OrthoDBiPOG091H0UB7

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

O66884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEFLELDGS YGEGGGQIVR TALFLSTLLQ VPVRIYNIRA KREKPGLKRQ
60 70 80 90 100
HLNIVKLLKR FAKAKVKGDE LGSIELEYKP QKLKPGYYEV NFDTAGSIPL
110 120 130 140 150
FLQTILPLSL FVPGEVVIKV IGGTDVPKSP TIDWVRFVFL HYIYKVPEFI
160 170 180 190 200
RLEVLKRGFY PAGGGIVQLS VRNKLEKQPE NLQEVKEYLK DKVNLYRTER
210 220 230 240 250
GEIRKIHVFS VAHEELRERK VAERQAKGAI EALKNLGYPE PEVYRQYANA
260 270 280 290 300
NSIGTSITIW LEDTKGNVLG ADNLGKKGKL AEVVGAEAVK KLHEDWSSGA
310 320 330 340 350
TVDRHLADHL IPLMALVGGE IRVPQFTGHL LTNIWVCEQF LGKIFEVDEK
360
EKIVRTQGIS
Length:360
Mass (Da):40,583
Last modified:August 1, 1998 - v1
Checksum:i889997AFD139F776
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06852.1
PIRiH70356
RefSeqiNP_213444.1, NC_000918.1
WP_010880382.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC06852; AAC06852; aq_642
GeneIDi1193946
KEGGiaae:aq_642
PATRICifig|224324.8.peg.523

Similar proteinsi

Entry informationi

Entry nameiRTCA_AQUAE
AccessioniPrimary (citable) accession number: O66884
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health