Reviewed, UniProtKB/Swiss-Prot O66884 (RTCA_AQUAE)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	aq_642

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156415
Sites
	Active site	329	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66884-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 889997AFD139F776	FASTA	360	40,583
	10 20 30 40 50 60 MGEFLELDGS YGEGGGQIVR TALFLSTLLQ VPVRIYNIRA KREKPGLKRQ HLNIVKLLKR 70 80 90 100 110 120 FAKAKVKGDE LGSIELEYKP QKLKPGYYEV NFDTAGSIPL FLQTILPLSL FVPGEVVIKV 130 140 150 160 170 180 IGGTDVPKSP TIDWVRFVFL HYIYKVPEFI RLEVLKRGFY PAGGGIVQLS VRNKLEKQPE 190 200 210 220 230 240 NLQEVKEYLK DKVNLYRTER GEIRKIHVFS VAHEELRERK VAERQAKGAI EALKNLGYPE 250 260 270 280 290 300 PEVYRQYANA NSIGTSITIW LEDTKGNVLG ADNLGKKGKL AEVVGAEAVK KLHEDWSSGA 310 320 330 340 350 360 TVDRHLADHL IPLMALVGGE IRVPQFTGHL LTNIWVCEQF LGKIFEVDEK EKIVRTQGIS

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06852.1.
PIR	H70356.
RefSeq	NP_213444.1.
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Genome annotation databases
GeneID	1193946.
GenomeReviews	Gene locus aq_642 in contig AE000657_GR.
KEGG	aae:aq_642.
NMPDR	fig|224324.1.peg.459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66884.
OMA	FNIGTAG.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_642-MON.
BRENDA	6.5.1.4. 189781.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_AQUAE

Accession

Primary (citable) accession number: O66884

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)