Penicillin-binding protein 1A

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Reviewed, UniProtKB/Swiss-Prot O66874 (PBPA_AQUAE)

Last modified June 16, 2009. Version 68. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Penicillin-binding protein 1A
      Short name=PBP-1a
      Short name=PBP1a
Including the following 2 domains:
    1- Recommended name:
            Penicillin-insensitive transglycosylase
              EC=2.4.2.-
        Alternative name(s):
            Peptidoglycan TGase
    2- Recommended name:
            Penicillin-sensitive transpeptidase
              EC=3.4.-.-
        Alternative name(s):
            DD-transpeptidase

Gene names

Name:	mrcA
Synonyms:	ponA
Ordered Locus Names:	aq_624

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

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Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell inner membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 51 family.

In the C-terminal section; belongs to the transpeptidase family.

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Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Hydrolase Transferase
Technical term	3D-structure Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan-based cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	penicillin binding Inferred from electronic annotation. Source: InterPro peptidase activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 726

726

Penicillin-binding protein 1A

PRO_0000083178

Regions

Topological domain

1 – 3

Cytoplasmic Potential

Transmembrane

4 – 24

Signal-anchor for type II membrane protein Potential

Topological domain

25 – 726

702

Periplasmic Potential

Region

45 – 213

169

Transglycosylase

Region

379 – 662

284

Transpeptidase

Sites

Active site

432

Acyl-ester intermediate By similarity

Secondary structure

726

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

O66874-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 37F756397C9D7B38
Show »

FASTA

726

81,824

        10         20         30         40         50         60 
MKKLVIGILG IVIALFVGLL VFLIPIYKNL PDPKLLESWT PPQASEVYDA KGRLYGTIGI 

        70         80         90        100        110        120 
QKRFYVSIDK IPEHVINAFV ATEDRNFWHH FGIDPVAIVR AAIVNYRAGR IVQGGSTITQ 

       130        140        150        160        170        180 
QLAKNLFLTR ERTLERKIKE ALLAIKIERT FDKKKIMELY LNQIYLGSGA YGVEAAAQVY 

       190        200        210        220        230        240 
FGKHVWELSL DEAALLAALP KAPAKYNPFY HPERALQRRN LVLKRMLEEG YITPEQYEEA 

       250        260        270        280        290        300 
VNKPLTVKKE NKYKFSDYFL DMVKSYVFNK YGEIAYKGRL KIYTTIDLDY QKIAQKSLEE 

       310        320        330        340        350        360 
GLKRVAKIIG LPFLPKSEED MELAYEKEAQ LKRLKRGKIY VAKILKYDGN FMKVEIHGKK 

       370        380        390        400        410        420 
LKGEIKGLNT EGHKYVFVKY LGGNRAEIIP DLEGSLVSID VKTGEIKAIV GGRSYAYSQF 

       430        440        450        460        470        480 
NRAVKALRQP GSAIKPVIYL SALLKGMTQI STIDASSKPY YDPSKGEDWI PKNYDEKEYG 

       490        500        510        520        530        540 
NVTLRYALAH SINTAAVNLL DKVGFELVLE VGKKVGLDNL KPYYSLALGT VEVTPLQLTA 

       550        560        570        580        590        600 
AYQVFANLGT ECKPFFIKKI VDENGEVLEE NVPECEEVLP KPETRVPVDM LRAVVLEGTA 

       610        620        630        640        650        660 
RRASVLDRIV AGKTGTTDDF QDAWFVGFSP YIVTGVWVGY DVKKSLGKHM SGSRVALPIW 

       670        680        690        700        710        720 
IDYMKVVTRM YPNEDFELPP ENIVVNINPK DLVLADETCE GVPMVFVKGT EPHITCSDLN 


AILGLR

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References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

AE000657 Genomic DNA. Translation: AAC06835.1.

PIR

F70355.

RefSeq

NP_213434.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OQO	X-ray	2.10	A	51-243	[»]
3D3H	X-ray	2.31	A	51-243	[»]

ModBase

Search...

Protein family/group databases

CAZy

GT51. Glycosyltransferase Family 51.

Genome annotation databases

GeneID

1193936.

GenomeReviews

Gene locus aq_624 in contig AE000657_GR.

KEGG

aae:aq_624.

NMPDR

fig|224324.1.peg.449.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O66874.

OMA

O66874. GTDEVTL.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_624-MON.

Family and domain databases

InterPro

IPR001264. Glyco_trans_51.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]

Pfam

PF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]

ProDom

PD001895. Glyco_trans_51. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR02074. PBP_1a_fam. 1 hit.

ProtoNet

Search...

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Entry information

Entry name

PBPA_AQUAE

Accession

Primary (citable) accession number: O66874

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families