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O66874 (PBPA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Penicillin-binding protein 1A
Short name=PBP-1a
Short name=PBP1a

Including the following 2 domains:

  1. Penicillin-insensitive transglycosylase
    EC=2.4.2.-
    Alternative name(s):
    Peptidoglycan TGase
  2. Penicillin-sensitive transpeptidase
    EC=3.4.-.-
    Alternative name(s):
    DD-transpeptidase
Gene names
Name:mrcA
Synonyms:ponA
Ordered Locus Names:aq_624
OrganismAquifex aeolicus (strain VF5)
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length726 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell inner membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 51 family.

In the C-terminal section; belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 726726Penicillin-binding protein 1A
PRO_0000083178

Regions

Topological domain1 – 33Cytoplasmic Potential
Transmembrane4 – 2421Helical; Signal-anchor for type II membrane protein; Potential
Topological domain25 – 726702Periplasmic Potential
Region45 – 213169Transglycosylase
Region379 – 662284Transpeptidase

Sites

Active site4321Acyl-ester intermediate By similarity

Secondary structure

1 .................................. 726
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O66874 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 37F756397C9D7B38

FASTA72681,824
        10         20         30         40         50         60 
MKKLVIGILG IVIALFVGLL VFLIPIYKNL PDPKLLESWT PPQASEVYDA KGRLYGTIGI 

        70         80         90        100        110        120 
QKRFYVSIDK IPEHVINAFV ATEDRNFWHH FGIDPVAIVR AAIVNYRAGR IVQGGSTITQ 

       130        140        150        160        170        180 
QLAKNLFLTR ERTLERKIKE ALLAIKIERT FDKKKIMELY LNQIYLGSGA YGVEAAAQVY 

       190        200        210        220        230        240 
FGKHVWELSL DEAALLAALP KAPAKYNPFY HPERALQRRN LVLKRMLEEG YITPEQYEEA 

       250        260        270        280        290        300 
VNKPLTVKKE NKYKFSDYFL DMVKSYVFNK YGEIAYKGRL KIYTTIDLDY QKIAQKSLEE 

       310        320        330        340        350        360 
GLKRVAKIIG LPFLPKSEED MELAYEKEAQ LKRLKRGKIY VAKILKYDGN FMKVEIHGKK 

       370        380        390        400        410        420 
LKGEIKGLNT EGHKYVFVKY LGGNRAEIIP DLEGSLVSID VKTGEIKAIV GGRSYAYSQF 

       430        440        450        460        470        480 
NRAVKALRQP GSAIKPVIYL SALLKGMTQI STIDASSKPY YDPSKGEDWI PKNYDEKEYG 

       490        500        510        520        530        540 
NVTLRYALAH SINTAAVNLL DKVGFELVLE VGKKVGLDNL KPYYSLALGT VEVTPLQLTA 

       550        560        570        580        590        600 
AYQVFANLGT ECKPFFIKKI VDENGEVLEE NVPECEEVLP KPETRVPVDM LRAVVLEGTA 

       610        620        630        640        650        660 
RRASVLDRIV AGKTGTTDDF QDAWFVGFSP YIVTGVWVGY DVKKSLGKHM SGSRVALPIW 

       670        680        690        700        710        720 
IDYMKVVTRM YPNEDFELPP ENIVVNINPK DLVLADETCE GVPMVFVKGT EPHITCSDLN 


AILGLR

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References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC06835.1.
PIRF70355.
RefSeqNP_213434.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2OQOX-ray2.10A51-243[»]
3D3HX-ray2.31A51-243[»]
3NB6X-ray2.70A51-243[»]
3NB7X-ray2.65A51-243[»]
ProteinModelPortalO66874.
SMRO66874. Positions 57-243.
ModBaseSearch...

Protein family/group databases

CAZyGT51. Glycosyltransferase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1193936.
GenomeReviewsGene locus aq_624 in contig AE000657_GR.
KEGGaae:aq_624.
NMPDRfig|224324.1.peg.449.
PATRIC20958748. VBIAquAeo85532_0507.

Phylogenomic databases

HOGENOMHBG672432.
OMANISECAL.
PhylomeDBO66874.
ProtClustDBCLSK2299450.

Enzyme and pathway databases

BioCycAAEO224324:AQ_624-MONOMER.

Family and domain databases

InterProIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
Gene3DG3DSA:3.40.710.10. G3DSA:3.40.710.10. 2 hits.
KOK05366.
PfamPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56601. PBP_transp_fold. 1 hit.
TIGRFAMsTIGR02074. PBP_1a_fam. 1 hit.
ProtoNetSearch...

Entry information

Entry namePBPA_AQUAE
AccessionPrimary (citable) accession number: O66874
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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