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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.By similarity
Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83Proton donor; for transglycosylase activityBy similarity1
Active sitei432Acyl-ester intermediate; for transpeptidase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase
Biological processAntibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-452-MONOMER
BRENDAi2.4.1.129 396
UniPathwayiUPA00219

Protein family/group databases

CAZyiGT51 Glycosyltransferase Family 51

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129By similarity)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.16.4By similarity)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:aq_624
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini25 – 726PeriplasmicSequence analysisAdd BLAST702

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831781 – 726Penicillin-binding protein 1AAdd BLAST726

Proteomic databases

PRIDEiO66874

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15625175,EBI-15625175

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-60897N
STRINGi224324.aq_624

Structurei

Secondary structure

1726
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi60 – 62Combined sources3
Helixi68 – 70Combined sources3
Helixi73 – 83Combined sources11
Turni85 – 89Combined sources5
Beta strandi90 – 93Combined sources4
Helixi95 – 102Combined sources8
Helixi118 – 124Combined sources7
Beta strandi127 – 129Combined sources3
Helixi134 – 150Combined sources17
Helixi153 – 163Combined sources11
Helixi173 – 181Combined sources9
Helixi185 – 187Combined sources3
Helixi190 – 198Combined sources9
Helixi203 – 206Combined sources4
Turni208 – 210Combined sources3
Helixi212 – 228Combined sources17
Helixi234 – 241Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQOX-ray2.10A51-243[»]
3D3HX-ray2.31A51-243[»]
3NB6X-ray2.70A51-243[»]
3NB7X-ray2.65A51-243[»]
ProteinModelPortaliO66874
SMRiO66874
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66874

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 213TransglycosylaseAdd BLAST169
Regioni379 – 662TranspeptidaseAdd BLAST284

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JQC Bacteria
COG5009 LUCA
HOGENOMiHOG000041137
InParanoidiO66874
KOiK05366
OMAiLAQMAMI
OrthoDBiPOG091H01NC

Family and domain databases

Gene3Di1.10.3810.10, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR001264 Glyco_trans_51
IPR023346 Lysozyme-like_dom_sf
IPR036950 PBP_transglycosylase
IPR001460 PCN-bd_Tpept
PfamiView protein in Pfam
PF00912 Transgly, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF56601 SSF56601, 3 hits

Sequencei

Sequence statusi: Complete.

O66874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLVIGILG IVIALFVGLL VFLIPIYKNL PDPKLLESWT PPQASEVYDA
60 70 80 90 100
KGRLYGTIGI QKRFYVSIDK IPEHVINAFV ATEDRNFWHH FGIDPVAIVR
110 120 130 140 150
AAIVNYRAGR IVQGGSTITQ QLAKNLFLTR ERTLERKIKE ALLAIKIERT
160 170 180 190 200
FDKKKIMELY LNQIYLGSGA YGVEAAAQVY FGKHVWELSL DEAALLAALP
210 220 230 240 250
KAPAKYNPFY HPERALQRRN LVLKRMLEEG YITPEQYEEA VNKPLTVKKE
260 270 280 290 300
NKYKFSDYFL DMVKSYVFNK YGEIAYKGRL KIYTTIDLDY QKIAQKSLEE
310 320 330 340 350
GLKRVAKIIG LPFLPKSEED MELAYEKEAQ LKRLKRGKIY VAKILKYDGN
360 370 380 390 400
FMKVEIHGKK LKGEIKGLNT EGHKYVFVKY LGGNRAEIIP DLEGSLVSID
410 420 430 440 450
VKTGEIKAIV GGRSYAYSQF NRAVKALRQP GSAIKPVIYL SALLKGMTQI
460 470 480 490 500
STIDASSKPY YDPSKGEDWI PKNYDEKEYG NVTLRYALAH SINTAAVNLL
510 520 530 540 550
DKVGFELVLE VGKKVGLDNL KPYYSLALGT VEVTPLQLTA AYQVFANLGT
560 570 580 590 600
ECKPFFIKKI VDENGEVLEE NVPECEEVLP KPETRVPVDM LRAVVLEGTA
610 620 630 640 650
RRASVLDRIV AGKTGTTDDF QDAWFVGFSP YIVTGVWVGY DVKKSLGKHM
660 670 680 690 700
SGSRVALPIW IDYMKVVTRM YPNEDFELPP ENIVVNINPK DLVLADETCE
710 720
GVPMVFVKGT EPHITCSDLN AILGLR
Length:726
Mass (Da):81,824
Last modified:August 1, 1998 - v1
Checksum:i37F756397C9D7B38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06835.1
PIRiF70355
RefSeqiNP_213434.1, NC_000918.1
WP_010880372.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC06835; AAC06835; aq_624
GeneIDi1193936
KEGGiaae:aq_624
PATRICifig|224324.8.peg.507

Similar proteinsi

Entry informationi

Entry nameiPBPA_AQUAE
AccessioniPrimary (citable) accession number: O66874
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health