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Protein

Penicillin-binding protein 1A

Gene

mrcA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei432Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BRENDAi2.4.1.129. 396.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP-1a
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcA
Synonyms:ponA
Ordered Locus Names:aq_624
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini25 – 726PeriplasmicSequence analysisAdd BLAST702

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831781 – 726Penicillin-binding protein 1AAdd BLAST726

Interactioni

Protein-protein interaction databases

DIPiDIP-60897N.
STRINGi224324.aq_624.

Structurei

Secondary structure

1726
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi60 – 62Combined sources3
Helixi68 – 70Combined sources3
Helixi73 – 83Combined sources11
Turni85 – 89Combined sources5
Beta strandi90 – 93Combined sources4
Helixi95 – 102Combined sources8
Helixi118 – 124Combined sources7
Beta strandi127 – 129Combined sources3
Helixi134 – 150Combined sources17
Helixi153 – 163Combined sources11
Helixi173 – 181Combined sources9
Helixi185 – 187Combined sources3
Helixi190 – 198Combined sources9
Helixi203 – 206Combined sources4
Turni208 – 210Combined sources3
Helixi212 – 228Combined sources17
Helixi234 – 241Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQOX-ray2.10A51-243[»]
3D3HX-ray2.31A51-243[»]
3NB6X-ray2.70A51-243[»]
3NB7X-ray2.65A51-243[»]
ProteinModelPortaliO66874.
SMRiO66874.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66874.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 213TransglycosylaseAdd BLAST169
Regioni379 – 662TranspeptidaseAdd BLAST284

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
HOGENOMiHOG000041137.
InParanoidiO66874.
KOiK05366.
OMAiAMSENAR.
OrthoDBiPOG091H01NC.

Family and domain databases

Gene3Di3.40.710.10. 2 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 3 hits.

Sequencei

Sequence statusi: Complete.

O66874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLVIGILG IVIALFVGLL VFLIPIYKNL PDPKLLESWT PPQASEVYDA
60 70 80 90 100
KGRLYGTIGI QKRFYVSIDK IPEHVINAFV ATEDRNFWHH FGIDPVAIVR
110 120 130 140 150
AAIVNYRAGR IVQGGSTITQ QLAKNLFLTR ERTLERKIKE ALLAIKIERT
160 170 180 190 200
FDKKKIMELY LNQIYLGSGA YGVEAAAQVY FGKHVWELSL DEAALLAALP
210 220 230 240 250
KAPAKYNPFY HPERALQRRN LVLKRMLEEG YITPEQYEEA VNKPLTVKKE
260 270 280 290 300
NKYKFSDYFL DMVKSYVFNK YGEIAYKGRL KIYTTIDLDY QKIAQKSLEE
310 320 330 340 350
GLKRVAKIIG LPFLPKSEED MELAYEKEAQ LKRLKRGKIY VAKILKYDGN
360 370 380 390 400
FMKVEIHGKK LKGEIKGLNT EGHKYVFVKY LGGNRAEIIP DLEGSLVSID
410 420 430 440 450
VKTGEIKAIV GGRSYAYSQF NRAVKALRQP GSAIKPVIYL SALLKGMTQI
460 470 480 490 500
STIDASSKPY YDPSKGEDWI PKNYDEKEYG NVTLRYALAH SINTAAVNLL
510 520 530 540 550
DKVGFELVLE VGKKVGLDNL KPYYSLALGT VEVTPLQLTA AYQVFANLGT
560 570 580 590 600
ECKPFFIKKI VDENGEVLEE NVPECEEVLP KPETRVPVDM LRAVVLEGTA
610 620 630 640 650
RRASVLDRIV AGKTGTTDDF QDAWFVGFSP YIVTGVWVGY DVKKSLGKHM
660 670 680 690 700
SGSRVALPIW IDYMKVVTRM YPNEDFELPP ENIVVNINPK DLVLADETCE
710 720
GVPMVFVKGT EPHITCSDLN AILGLR
Length:726
Mass (Da):81,824
Last modified:August 1, 1998 - v1
Checksum:i37F756397C9D7B38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06835.1.
PIRiF70355.
RefSeqiNP_213434.1. NC_000918.1.
WP_010880372.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06835; AAC06835; aq_624.
GeneIDi1193936.
KEGGiaae:aq_624.
PATRICi20958748. VBIAquAeo85532_0507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06835.1.
PIRiF70355.
RefSeqiNP_213434.1. NC_000918.1.
WP_010880372.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQOX-ray2.10A51-243[»]
3D3HX-ray2.31A51-243[»]
3NB6X-ray2.70A51-243[»]
3NB7X-ray2.65A51-243[»]
ProteinModelPortaliO66874.
SMRiO66874.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60897N.
STRINGi224324.aq_624.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06835; AAC06835; aq_624.
GeneIDi1193936.
KEGGiaae:aq_624.
PATRICi20958748. VBIAquAeo85532_0507.

Phylogenomic databases

eggNOGiENOG4108JQC. Bacteria.
COG5009. LUCA.
HOGENOMiHOG000041137.
InParanoidiO66874.
KOiK05366.
OMAiAMSENAR.
OrthoDBiPOG091H01NC.

Enzyme and pathway databases

UniPathwayiUPA00219.
BRENDAi2.4.1.129. 396.

Miscellaneous databases

EvolutionaryTraceiO66874.

Family and domain databases

Gene3Di3.40.710.10. 2 hits.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPBPA_AQUAE
AccessioniPrimary (citable) accession number: O66874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.