Reviewed, UniProtKB/Swiss-Prot O66862 (LPXA_AQUAE)

Names and origin

Protein names

Recommended name:
    Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
      Short name=UDP-N-acetylglucosamine acyltransferase
    EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	aq_604

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity.
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_0000188033

Sequences

	Sequence		Length	Mass (Da)
	O66862-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: E101ADD6B5122961	FASTA	261	28,416
	10 20 30 40 50 60 MKVHSSVLIE GEVEIPEDVE IGAYTVIQGN VKIGKGTKIG NRVTIKGNVT IGENCKIFDG 70 80 90 100 110 120 AVIGEAPQHL KYEGEETSVE IGNNVIIREY VTIHRGTKLD KGKTVVGDNV MLMAYSHVAH 130 140 150 160 170 180 DCVVGNNVIM ANCATLGGHV VVGDYALIGG LSAVHQWARV GEHAMVGGLT GVSLDIPPYT 190 200 210 220 230 240 VASGQHAKLY GINIIGLRRR GFPEEVIKAI SKAYRIIFRS PLPRQKAPEI VFQELGQYEE 250 260 VRKMVEFIKS SKRGVARHHK D

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06825.1.
PIR	B70354.
RefSeq	NP_213422.1.
3D structure databases
HSSP	HSSP built from PDB template 1LXA based on UniProtKB P10440.
ModBase	Search...
Genome annotation databases
GeneID	1193924.
GenomeReviews	Gene locus aq_604 in contig AE000657_GR.
KEGG	aae:aq_604.
NMPDR	fig|224324.1.peg.437.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66862.
OMA	DLKYQGE.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_604-MON.
BRENDA	2.3.1.129. 189781.
Family and domain databases
HAMAP	MF_00387. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010137. Lipid_A_lpxA. [Graphical view]
Pfam	PF00132. Hexapep. 7 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_AQUAE

Accession

Primary (citable) accession number: O66862

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)