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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain 1 (trpB1), Tryptophan synthase beta chain 2 (trpB2)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52TryptophanBy similarity1
Metal bindingi336MagnesiumBy similarity1
Binding sitei424ChorismateBy similarity1
Binding sitei444ChorismateBy similarity1
Binding sitei460Chorismate; via amide nitrogenBy similarity1
Metal bindingi473MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:aq_582
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540741 – 494Anthranilate synthase component 1Add BLAST494

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Protein-protein interaction databases

STRINGi224324.aq_582.

Structurei

3D structure databases

ProteinModelPortaliO66849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 276Tryptophan bindingBy similarity3
Regioni309 – 310Chorismate bindingBy similarity2
Regioni458 – 460Chorismate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
HOGENOMiHOG000025142.
InParanoidiO66849.
KOiK01657.
OMAiCEELAVI.
OrthoDBiPOG091H01VQ.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.

Sequencei

Sequence statusi: Complete.

O66849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLNLSLEEV RKLSENYNVI PLYTELLVDT ETPLSIFLKL KEKGQFNILL
60 70 80 90 100
ESAEGGEKWG RYSFIITGSS FYLRTRKDIG EIYERGKVNF FETKDPLSKI
110 120 130 140 150
KEVVKKFIPY HDERLPRFWG GLVGYFAYDV VKFYEPVEDK NPDPIHTYDI
160 170 180 190 200
YLVLTDVVVI HDNLTGKIKV VVPIFAQNGI EEEYERAKNL IRDTVKKLKE
210 220 230 240 250
RGTTFLNVVE KEPDFKNWRS NFTKEEFEDI VKKAKEYIAQ GDVIQVVLSQ
260 270 280 290 300
RFRKRFKGNP DNIYRVLRFL NPSPYMYYLD FDQLKVIGSS PEILVRLEEG
310 320 330 340 350
RIETRPIAGT RKRGRTEEED KRLEEDLLSD EKERAEHLML VDLARNDIGR
360 370 380 390 400
VAKTGSVRVE NFMRIERYSH VMHIVSDVVG ELREGYDALD VLKATFPAGT
410 420 430 440 450
VSGAPKVRAM QIIEELENER RGIYAGSVGY ISFQGNMDMA IAIRTAVYRD
460 470 480 490
RDIFVQAGAG IVADSVPEKE WEETVNKAKA LMKAIEIAEE SQEE
Length:494
Mass (Da):57,087
Last modified:August 1, 1998 - v1
Checksum:iAC20BBB260867CEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06796.1.
PIRiE70352.
RefSeqiNP_213409.1. NC_000918.1.
WP_010880347.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06796; AAC06796; aq_582.
GeneIDi1193267.
KEGGiaae:aq_582.
PATRICi20958684. VBIAquAeo85532_0476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06796.1.
PIRiE70352.
RefSeqiNP_213409.1. NC_000918.1.
WP_010880347.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06796; AAC06796; aq_582.
GeneIDi1193267.
KEGGiaae:aq_582.
PATRICi20958684. VBIAquAeo85532_0476.

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
HOGENOMiHOG000025142.
InParanoidiO66849.
KOiK01657.
OMAiCEELAVI.
OrthoDBiPOG091H01VQ.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPE_AQUAE
AccessioniPrimary (citable) accession number: O66849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.