Reviewed, UniProtKB/Swiss-Prot O66847 (DEF_AQUAE)

Names and origin

Protein names

Recommended name:
    Peptide deformylase
      Short name=PDF
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase

Gene names

Name:	def
Ordered Locus Names:	aq_579

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Peptide deformylase HAMAP MF_00163		PRO_0000082732
Sites
	Active site	136	1	By similarity
	Metal binding	93	1	Iron By similarity
	Metal binding	135	1	Iron By similarity
	Metal binding	139	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66847-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2C20014DFB199B6E	FASTA	169	19,270
	10 20 30 40 50 60 MVRDIVIYPN EILKKPTEKV DVIDKEVKNL IRDMFDTMYE AEGVGLAANQ IGVPLSVMVI 70 80 90 100 110 120 DTSPKEDAPP LKLVLINPEI KEGEGKIKYK EGCLSFPGLS VEVERFQKVK VNALNEHGEP 130 140 150 160 VELTLEGFPA IVFQHELDHL KGITFVDRLK GWRRRMALEK YQKLLKSRK

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06802.1.
PIR	C70352.
RefSeq	NP_213407.1.
3D structure databases
HSSP	HSSP built from PDB template 2DEF based on UniProtKB P27251.
ModBase	Search...
Genome annotation databases
GeneID	1193265.
GenomeReviews	Gene locus aq_579 in contig AE000657_GR.
KEGG	aae:aq_579.
NMPDR	fig|224324.1.peg.422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66847.
OMA	O66847. HEEASPN.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_579-MON.
BRENDA	3.5.1.88. 189781.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF_AQUAE

Accession

Primary (citable) accession number: O66847

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)