O66842 (NUOE_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit E
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit E
NDH-1 subunit E

Gene names

Name:	nuoE
Ordered Locus Names:	aq_574

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from sequence or structural similarity. Source: RefGenome
Cellular component	mitochondrial respiratory chain complex I Inferred from sequence or structural similarity. Source: RefGenome
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from sequence or structural similarity. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 160	160	NADH-quinone oxidoreductase subunit E		PRO_0000118687
Sites
	Metal binding	86	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	91	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	127	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	131	1	Iron-sulfur (2Fe-2S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O66842 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 425D81995A491B3B	FASTA	160	18,551
	10 20 30 40 50 60 MFKTEFEFPE ELKTKLQEHI NYFPKKRQAI LLCLHEIQNY YGYIPPESLK PLADMLELPL 70 80 90 100 110 120 NHVEGVVAFY DMFDREDKAK YRIRVCVSIV CHLMGTNKLL KALENILGIK PGEVTPDGKF 130 140 150 160 KIVPVQCLGA CSEAPVFMVN DDEYKFESEV QLNEILSRYT

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06799.1.
PIR	F70351.
RefSeq	NP_213402.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66842.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193260.
GenomeReviews	Gene locus aq_574 in contig AE000657_GR.
KEGG	aae:aq_574.
NMPDR	fig|224324.1.peg.417.
PATRIC	20958670. VBIAquAeo85532_0469.
Phylogenomic databases
HOGENOM	HBG616790.
OMA	RETECLA.
PhylomeDB	O66842.
ProtClustDB	CLSK2299435.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_574-MONOMER.
Family and domain databases
InterPro	IPR002023. NADH_UbQ_OxRdtase_24kDa_su. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00334.
PANTHER	PTHR10371. Cmplx1_24kDa. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
PIRSF	PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. NuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOE_AQUAE

Accession

Primary (citable) accession number: O66842

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program