O66841 (NUOF_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit F
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	aq_573

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	NADH-quinone oxidoreductase subunit F		PRO_0000118570
Regions
	Nucleotide binding	65 – 74	10	NAD By similarity
	Nucleotide binding	176 – 223	48	FMN By similarity
Sites
	Metal binding	347	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	350	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	353	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	393	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O66841 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: A876712CBFA2E122	FASTA	426	47,508
	10 20 30 40 50 60 MRSYPAIPRI YAETTLNMLL KRAKKPRVHS IDEYLKDGGY QALEKALNMS PEEIIDWVDK 70 80 90 100 110 120 STLRGRGGAG FPTGKKWKFA VQNPGPRYFI CNADESEPGT FKDRIIIERD PHLLIEGIII 130 140 150 160 170 180 SSYAIGANEA YIYIRGEYPA GYYILRDAIE EAKKKGFLGK NILGSGFDLE IYVARGAGAY 190 200 210 220 230 240 ICGEETALIE SLEGKRGHPR LKPPYPVQKG LWGKPTVVNN VETIANVPFI ISMGWEEYRY 250 260 270 280 290 300 IGPSDYAGPK LFPVSGKVKK PGVYELPMNT TLREVIFKYA GGTLGNKKVK AVFSGALDCF 310 320 330 340 350 360 SSEELDIPMD YSPLGFGGTG TVIVLTEEDD IVEAALKIAE FYEHETCGQC TPCRVGCYEQ 370 380 390 400 410 420 ANLLEKIYKG EATEQDWEGF DFVNRNIQPT SICGLGAVAG RLIRQTLEKF PEEWEKYRKK SASLPL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06800.1.
PIR	E70351.
RefSeq	NP_213401.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66841.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193259.
GenomeReviews	Gene locus aq_573 in contig AE000657_GR.
KEGG	aae:aq_573.
NMPDR	fig|224324.1.peg.416.
PATRIC	20958668. VBIAquAeo85532_0468.
Phylogenomic databases
HOGENOM	HBG669338.
OMA	WAGGPLE.
PhylomeDB	O66841.
ProtClustDB	CLSK2299434.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_573-MONOMER.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
KO	K00335.
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_AQUAE

Accession

Primary (citable) accession number: O66841

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program