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O66839 (PNPH_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 6-oxopurine nucleoside phosphorylase

EC=2.4.2.1
Alternative name(s):
Purine nucleoside phosphorylase
Short name=PNP
Gene names
Ordered Locus Names:aq_568
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity. HAMAP-Rule MF_01963

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 277277Probable 6-oxopurine nucleoside phosphorylase HAMAP-Rule MF_01963
PRO_0000415080

Regions

Region50 – 512Phosphate binding By similarity
Region83 – 842Phosphate binding By similarity
Region223 – 2253Substrate binding By similarity

Sites

Binding site81Phosphate By similarity
Binding site1991Substrate; via amide nitrogen By similarity
Binding site2001Phosphate By similarity
Site1811Important for substrate specificity By similarity
Site2351Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
O66839 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 1C90A95984B0B04E

FASTA27730,812
        10         20         30         40         50         60 
MLGIIGGSGL YNLPGIKVKE EVQVKTPFGE PSSPVVIAEV EGKKVAFLAR HGRGHEYPPH 

        70         80         90        100        110        120 
LVPYRANLWA LREVGVKRVL GISAVGGINE LLMPGDFVVI HDYLDFTKTR RSTYYEGKFS 

       130        140        150        160        170        180 
VKVEGEDKVA KLLREGKVVH VDMSEAYCPE MRKVLIQILK EKNFRFHPKG VYACTEGPRF 

       190        200        210        220        230        240 
ETPSEIKMLK LLGADVVGMT GYPEVALARE LTMCYASLCV VANPAAGIAG YRLTSNEVIQ 

       250        260        270 
LMKRKEEEIK EVVLKFIKEL PEIRSCDCEK SLEGAEV 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC06801.1.
PIRC70351.
RefSeqNP_213399.1. NC_000918.1.

3D structure databases

ProteinModelPortalO66839.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_568.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC06801; AAC06801; aq_568.
GeneID1193257.
KEGGaae:aq_568.
PATRIC20958662. VBIAquAeo85532_0465.

Phylogenomic databases

HOGENOMHOG000228987.
KOK00772.
OMAHVVGMTA.
OrthoDBEOG6KHFXC.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-420-MONOMER.
UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01694. MTAP. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNPH_AQUAE
AccessionPrimary (citable) accession number: O66839
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways