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O66839

- PNPH_AQUAE

UniProt

O66839 - PNPH_AQUAE

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Protein
Probable 6-oxopurine nucleoside phosphorylase
Gene
aq_568
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81Phosphate By similarity
Sitei181 – 1811Important for substrate specificity By similarity
Binding sitei199 – 1991Substrate; via amide nitrogen By similarity
Binding sitei200 – 2001Phosphate By similarity
Sitei235 – 2351Important for substrate specificity By similarity

GO - Molecular functioni

  1. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro
  2. phosphorylase activity Source: InterPro
  3. purine-nucleoside phosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-420-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylase (EC:2.4.2.1)
Alternative name(s):
Purine nucleoside phosphorylase
Short name:
PNP
Gene namesi
Ordered Locus Names:aq_568
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation
PRO_0000415080Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_568.

Structurei

3D structure databases

ProteinModelPortaliO66839.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 512Phosphate binding By similarity
Regioni83 – 842Phosphate binding By similarity
Regioni223 – 2253Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000228987.
KOiK00772.
OMAiHVVGMTA.
OrthoDBiEOG6KHFXC.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66839-1 [UniParc]FASTAAdd to Basket

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MLGIIGGSGL YNLPGIKVKE EVQVKTPFGE PSSPVVIAEV EGKKVAFLAR    50
HGRGHEYPPH LVPYRANLWA LREVGVKRVL GISAVGGINE LLMPGDFVVI 100
HDYLDFTKTR RSTYYEGKFS VKVEGEDKVA KLLREGKVVH VDMSEAYCPE 150
MRKVLIQILK EKNFRFHPKG VYACTEGPRF ETPSEIKMLK LLGADVVGMT 200
GYPEVALARE LTMCYASLCV VANPAAGIAG YRLTSNEVIQ LMKRKEEEIK 250
EVVLKFIKEL PEIRSCDCEK SLEGAEV 277
Length:277
Mass (Da):30,812
Last modified:August 1, 1998 - v1
Checksum:i1C90A95984B0B04E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06801.1.
PIRiC70351.
RefSeqiNP_213399.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06801; AAC06801; aq_568.
GeneIDi1193257.
KEGGiaae:aq_568.
PATRICi20958662. VBIAquAeo85532_0465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC06801.1 .
PIRi C70351.
RefSeqi NP_213399.1. NC_000918.1.

3D structure databases

ProteinModelPortali O66839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_568.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC06801 ; AAC06801 ; aq_568 .
GeneIDi 1193257.
KEGGi aae:aq_568.
PATRICi 20958662. VBIAquAeo85532_0465.

Phylogenomic databases

HOGENOMi HOG000228987.
KOi K00772.
OMAi HVVGMTA.
OrthoDBi EOG6KHFXC.

Enzyme and pathway databases

UniPathwayi UPA00606 .
BioCyci AAEO224324:GJBH-420-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
PROSITEi PS01240. PNP_MTAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPNPH_AQUAE
AccessioniPrimary (citable) accession number: O66839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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