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Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

aq_568

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81PhosphateUniRule annotation
Sitei181 – 1811Important for substrate specificityUniRule annotation
Binding sitei199 – 1991Substrate; via amide nitrogenUniRule annotation
Binding sitei200 – 2001PhosphateUniRule annotation
Sitei235 – 2351Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
  3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-420-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Alternative name(s):
Purine nucleoside phosphorylaseUniRule annotation
Short name:
PNPUniRule annotation
Gene namesi
Ordered Locus Names:aq_568
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277Probable 6-oxopurine nucleoside phosphorylasePRO_0000415080Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_568.

Structurei

3D structure databases

ProteinModelPortaliO66839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 512Phosphate bindingUniRule annotation
Regioni83 – 842Phosphate bindingUniRule annotation
Regioni223 – 2253Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228987.
InParanoidiO66839.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG6KHFXC.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66839-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGIIGGSGL YNLPGIKVKE EVQVKTPFGE PSSPVVIAEV EGKKVAFLAR
60 70 80 90 100
HGRGHEYPPH LVPYRANLWA LREVGVKRVL GISAVGGINE LLMPGDFVVI
110 120 130 140 150
HDYLDFTKTR RSTYYEGKFS VKVEGEDKVA KLLREGKVVH VDMSEAYCPE
160 170 180 190 200
MRKVLIQILK EKNFRFHPKG VYACTEGPRF ETPSEIKMLK LLGADVVGMT
210 220 230 240 250
GYPEVALARE LTMCYASLCV VANPAAGIAG YRLTSNEVIQ LMKRKEEEIK
260 270
EVVLKFIKEL PEIRSCDCEK SLEGAEV
Length:277
Mass (Da):30,812
Last modified:August 1, 1998 - v1
Checksum:i1C90A95984B0B04E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06801.1.
PIRiC70351.
RefSeqiNP_213399.1. NC_000918.1.
WP_010880337.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06801; AAC06801; aq_568.
GeneIDi1193257.
KEGGiaae:aq_568.
PATRICi20958662. VBIAquAeo85532_0465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06801.1.
PIRiC70351.
RefSeqiNP_213399.1. NC_000918.1.
WP_010880337.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO66839.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06801; AAC06801; aq_568.
GeneIDi1193257.
KEGGiaae:aq_568.
PATRICi20958662. VBIAquAeo85532_0465.

Phylogenomic databases

HOGENOMiHOG000228987.
InParanoidiO66839.
KOiK00772.
OMAiGCDIVGM.
OrthoDBiEOG6KHFXC.

Enzyme and pathway databases

UniPathwayiUPA00606.
BioCyciAAEO224324:GJBH-420-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPNPH_AQUAE
AccessioniPrimary (citable) accession number: O66839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: August 1, 1998
Last modified: February 4, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.