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O66839

- PNPH_AQUAE

UniProt

O66839 - PNPH_AQUAE

Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

aq_568

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81PhosphateUniRule annotation
    Sitei181 – 1811Important for substrate specificityUniRule annotation
    Binding sitei199 – 1991Substrate; via amide nitrogenUniRule annotation
    Binding sitei200 – 2001PhosphateUniRule annotation
    Sitei235 – 2351Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
    3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. purine ribonucleoside salvage Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-420-MONOMER.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
    Alternative name(s):
    Purine nucleoside phosphorylaseUniRule annotation
    Short name:
    PNPUniRule annotation
    Gene namesi
    Ordered Locus Names:aq_568
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 277277Probable 6-oxopurine nucleoside phosphorylasePRO_0000415080Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224324.aq_568.

    Structurei

    3D structure databases

    ProteinModelPortaliO66839.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 512Phosphate bindingUniRule annotation
    Regioni83 – 842Phosphate bindingUniRule annotation
    Regioni223 – 2253Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000228987.
    KOiK00772.
    OMAiHVVGMTA.
    OrthoDBiEOG6KHFXC.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O66839-1 [UniParc]FASTAAdd to Basket

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    MLGIIGGSGL YNLPGIKVKE EVQVKTPFGE PSSPVVIAEV EGKKVAFLAR    50
    HGRGHEYPPH LVPYRANLWA LREVGVKRVL GISAVGGINE LLMPGDFVVI 100
    HDYLDFTKTR RSTYYEGKFS VKVEGEDKVA KLLREGKVVH VDMSEAYCPE 150
    MRKVLIQILK EKNFRFHPKG VYACTEGPRF ETPSEIKMLK LLGADVVGMT 200
    GYPEVALARE LTMCYASLCV VANPAAGIAG YRLTSNEVIQ LMKRKEEEIK 250
    EVVLKFIKEL PEIRSCDCEK SLEGAEV 277
    Length:277
    Mass (Da):30,812
    Last modified:August 1, 1998 - v1
    Checksum:i1C90A95984B0B04E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06801.1.
    PIRiC70351.
    RefSeqiNP_213399.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC06801; AAC06801; aq_568.
    GeneIDi1193257.
    KEGGiaae:aq_568.
    PATRICi20958662. VBIAquAeo85532_0465.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC06801.1 .
    PIRi C70351.
    RefSeqi NP_213399.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O66839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_568.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC06801 ; AAC06801 ; aq_568 .
    GeneIDi 1193257.
    KEGGi aae:aq_568.
    PATRICi 20958662. VBIAquAeo85532_0465.

    Phylogenomic databases

    HOGENOMi HOG000228987.
    KOi K00772.
    OMAi HVVGMTA.
    OrthoDBi EOG6KHFXC.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .
    BioCyci AAEO224324:GJBH-420-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiPNPH_AQUAE
    AccessioniPrimary (citable) accession number: O66839
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3