Reviewed, UniProtKB/Swiss-Prot O66832 (BIOD_AQUAE)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	aq_557

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000187945
Regions
	Nucleotide binding	7 – 15	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66832-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9F5D2C28C5A0B907	FASTA	220	24,503
	10 20 30 40 50 60 MRLLITGTDT GVGKTFITYN LAKELKERGY KVGCLKPVET YVREVPEDGS LLSKATGQSV 70 80 90 100 110 120 NEIVPVRFSL PLAPYAATLE EGRDFALEEL GKHYEELSKK YKFLLVEGAG GIAVPIKKNY 130 140 150 160 170 180 TYANLARDWK LKVLIVGRAG LGTINHTFLT WYYAKATGLE VIGIILNGFT GEDVSERTNP 190 200 210 220 QIIEEMTGIK PYKVPRIQDV ELPKDIRTGL ADYVLSRFTP

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06785.1.
PIR	D70350.
RefSeq	NP_213392.1.
3D structure databases
HSSP	HSSP built from PDB template 1DAI based on UniProtKB P13000.
ModBase	Search...
Genome annotation databases
GeneID	1193250.
GenomeReviews	Gene locus aq_557 in contig AE000657_GR.
KEGG	aae:aq_557.
NMPDR	fig|224324.1.peg.407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66832.
OMA	VVIGSWP.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_557-MON.
BRENDA	6.3.3.3. 189781.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_AQUAE

Accession

Primary (citable) accession number: O66832

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)