O66832 (BIOD_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	aq_557

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187945
Regions
	Nucleotide binding	107 – 110	4	ATP By similarity
Sites
	Metal binding	15	1	Magnesium 2 By similarity
	Metal binding	48	1	Magnesium 2 By similarity
	Metal binding	107	1	Magnesium 2 By similarity
	Binding site	40	1	Substrate By similarity
	Binding site	48	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66832 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9F5D2C28C5A0B907	FASTA	220	24,503
	10 20 30 40 50 60 MRLLITGTDT GVGKTFITYN LAKELKERGY KVGCLKPVET YVREVPEDGS LLSKATGQSV 70 80 90 100 110 120 NEIVPVRFSL PLAPYAATLE EGRDFALEEL GKHYEELSKK YKFLLVEGAG GIAVPIKKNY 130 140 150 160 170 180 TYANLARDWK LKVLIVGRAG LGTINHTFLT WYYAKATGLE VIGIILNGFT GEDVSERTNP 190 200 210 220 QIIEEMTGIK PYKVPRIQDV ELPKDIRTGL ADYVLSRFTP

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06785.1.
PIR	D70350.
RefSeq	NP_213392.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66832.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193250.
GenomeReviews	Gene locus aq_557 in contig AE000657_GR.
KEGG	aae:aq_557.
NMPDR	fig|224324.1.peg.407.
PATRIC	20958646. VBIAquAeo85532_0458.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	ALAAYWQ.
PhylomeDB	O66832.
ProtClustDB	CLSK2748326.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_557-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_AQUAE

Accession

Primary (citable) accession number: O66832

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program