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O66806 (DDL_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:aq_521
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177780

Regions

Domain99 – 291193ATP-grasp
Nucleotide binding125 – 17955ATP By similarity

Sites

Metal binding2451Magnesium or manganese 1 By similarity
Metal binding2581Magnesium or manganese 1 By similarity
Metal binding2581Magnesium or manganese 2 By similarity
Metal binding2601Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
O66806 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: B0A892F15539C143

FASTA29132,797
        10         20         30         40         50         60 
MRVVVLMGGR SSEREISLKT GKAVAKALRE LGHEVYELDL DKELPCKLLE IKPDKVFIAL 

        70         80         90        100        110        120 
HGRYGEDGTV QGLLEILDIP YTGSDTIASA VSIDKDFTKR IVKSLGINTP DWETFISEGD 

       130        140        150        160        170        180 
VLNTEWNKFP AVVKPVREGS SVGLKIVESL EELKEYALDL LKKTERVMVE EFVEGRDMTV 

       190        200        210        220        230        240 
GILKGEALPV VEIIPKKGVY DYECKYTKGM SEYRILKDEK LSKKLQEISL KISKFLSLKD 

       250        260        270        280        290 
FARIDFRVTK EGKIFFLEVN TIPGMTELSL LPMAAKEKGM DFKKLISIII T 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC06765.1.
PIRB70347.
RefSeqNP_213366.1. NC_000918.1.

3D structure databases

ProteinModelPortalO66806.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC06765; AAC06765; aq_521.
GeneID1193224.
KEGGaae:aq_521.
PATRIC20958586. VBIAquAeo85532_0428.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBCLSK2299419.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-386-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_AQUAE
AccessionPrimary (citable) accession number: O66806
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways