O66806 (DDL_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Synonyms:	ddlA
Ordered Locus Names:	aq_521

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity HAMAP MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	D-alanine--D-alanine ligase HAMAP MF_00047		PRO_0000177780
Regions
	Domain	99 – 291	193	ATP-grasp
	Nucleotide binding	125 – 179	55	ATP By similarity
Sites
	Metal binding	245	1	Magnesium or manganese 1 By similarity
	Metal binding	258	1	Magnesium or manganese 1 By similarity
	Metal binding	258	1	Magnesium or manganese 2 By similarity
	Metal binding	260	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66806 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B0A892F15539C143	FASTA	291	32,797
	10 20 30 40 50 60 MRVVVLMGGR SSEREISLKT GKAVAKALRE LGHEVYELDL DKELPCKLLE IKPDKVFIAL 70 80 90 100 110 120 HGRYGEDGTV QGLLEILDIP YTGSDTIASA VSIDKDFTKR IVKSLGINTP DWETFISEGD 130 140 150 160 170 180 VLNTEWNKFP AVVKPVREGS SVGLKIVESL EELKEYALDL LKKTERVMVE EFVEGRDMTV 190 200 210 220 230 240 GILKGEALPV VEIIPKKGVY DYECKYTKGM SEYRILKDEK LSKKLQEISL KISKFLSLKD 250 260 270 280 290 FARIDFRVTK EGKIFFLEVN TIPGMTELSL LPMAAKEKGM DFKKLISIII T

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06765.1.
PIR	B70347.
RefSeq	NP_213366.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66806.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193224.
GenomeReviews	Gene locus aq_521 in contig AE000657_GR.
KEGG	aae:aq_521.
NMPDR	fig|224324.1.peg.381.
PATRIC	20958586. VBIAquAeo85532_0428.
Phylogenomic databases
HOGENOM	HBG427092.
OMA	TDGWYDY.
PhylomeDB	O66806.
ProtClustDB	CLSK2299419.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_521-MONOMER.
Family and domain databases
HAMAP	MF_00047. Dala_Dala_lig. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 2 hits. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01921.
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_AQUAE

Accession

Primary (citable) accession number: O66806

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program