Reviewed, UniProtKB/Swiss-Prot O66805 (MURB_AQUAE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	aq_520

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable HAMAP MF_00037.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179171
Regions
	Domain	16 – 182	167	FAD-binding PCMH-type
Sites
	Active site	162	1	By similarity
	Active site	210	1	Proton donor By similarity
	Active site	280	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66805-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 0E9BD1FDF938797B	FASTA	297	33,266
	10 20 30 40 50 60 MLFLKNVPLQ NLTTIKIGGR VSFYAEPSDL KEISLCIDFS KSRDIPLFVL GNGSNTIFGD 70 80 90 100 110 120 VRGLVVNLKN LKGFKVKEIK GKFFVEAFSG TPLKDLIRFS VKENVKSFYK LLGFPASVGG 130 140 150 160 170 180 AVSMNAGAFG VEISDFLKEV YFVDWEGKLQ KAKRDELNFS YRKSPFPKLG IVFKVVFEFE 190 200 210 220 230 240 RSKENILPKY EKIRRIRKEK QPINLPTSGS TFKNPEGNFA GKLLEKAGLK GFRLKNVGFS 250 260 270 280 290 EKHANFLVNY GGGTFSEVVD LINIAKERVY ENFGIVLEEE VKLIESSGSD GWKVLGA

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06766.1.
PIR	A70347.
RefSeq	NP_213365.1.
3D structure databases
SMR	O66805. Positions 4-285.
ModBase	Search...
Genome annotation databases
GeneID	1193223.
GenomeReviews	Gene locus aq_520 in contig AE000657_GR.
KEGG	aae:aq_520.
NMPDR	fig|224324.1.peg.380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686573.
OMA	EHEPLAG.
PhylomeDB	O66805.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_520-MONOMER.
BRENDA	1.1.1.158. 189781.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_AQUAE

Accession

Primary (citable) accession number: O66805

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)