Reviewed, UniProtKB/Swiss-Prot O66790 (TRXB_AQUAE)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
      Short name=TRXR
    EC=1.8.1.9

Gene names

Name:	trxB
Ordered Locus Names:	aq_500

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Thioredoxin reductase		PRO_0000166718
Regions
	Nucleotide binding	42 – 49	8	FAD By similarity
	Nucleotide binding	286 – 295	10	FAD By similarity
Amino acid modifications
	Disulfide bond	143 ↔ 146		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66790-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 363FC62EFDD03328	FASTA	323	35,101
	10 20 30 40 50 60 MAVSLMQQPD KVYDVIIIGA GPAGTTAAIY TARAGWKTLV LYRAEADGAL GVTQKIENYP 70 80 90 100 110 120 GVPGPLSGYE LLKIMREQAK SFGAEFVRGK VIATDLNSDP KKVYTIDGRE FRGKTIIVAS 130 140 150 160 170 180 GAMERANKFK GEEEFLGRGV SYCGVCDAAF FKDQPVAVIG DDDYAIEEAE FIARFANKVF 190 200 210 220 230 240 FVVPGSKIKA PPEVIEHFEK LPNVEILLRH RPIEIVGDQV VKGIKLKDLE KKEEKLLEVN 250 260 270 280 290 300 GVFIFLGGTK PSVDFLMGQV EMTEGDCIVV NEEMMTSVPG VFAAGDVLCN EVKQAVVAAA 310 320 MGCKAALAVD KFLSGKKKIV PQW

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06756.1.
PIR	B70345.
RefSeq	NP_213350.1.
3D structure databases
HSSP	HSSP built from PDB template 1TDE based on UniProtKB P09625.
ModBase	Search...
Genome annotation databases
GeneID	1193208.
GenomeReviews	Gene locus aq_500 in contig AE000657_GR.
KEGG	aae:aq_500.
NMPDR	fig|224324.1.peg.365.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66790.
OMA	IGDIRNT.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_500-MON.
BRENDA	1.8.1.9. 189781.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_AQUAE

Accession

Primary (citable) accession number: O66790

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)