O66776 (GLYA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	aq_479

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051 Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Pyridoxal-phosphate-dependent serine hydroxymethyltransferase		PRO_0000113522
Regions
	Region	121 – 123	3	Substrate binding By similarity
Sites
	Binding site	31	1	Pyridoxal phosphate By similarity
	Binding site	51	1	Pyridoxal phosphate By similarity
	Binding site	53	1	Substrate By similarity
	Binding site	60	1	Substrate binding By similarity
	Binding site	61	1	Pyridoxal phosphate By similarity
	Binding site	95	1	Pyridoxal phosphate By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	172	1	Pyridoxal phosphate By similarity
	Binding site	200	1	Pyridoxal phosphate By similarity
	Binding site	225	1	Pyridoxal phosphate By similarity
	Binding site	232	1	Pyridoxal phosphate By similarity
	Binding site	257	1	Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	357	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	226	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66776 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 883E1196CF472EE5	FASTA	428	47,454
	10 20 30 40 50 60 MEHLLKTDPE VFDAVVKEYE RQFYNLEMIA SENFTSLAVM EATGSVLTNK YAEGLPGKRY 70 80 90 100 110 120 YGGCEYVDVV ENLAIERAKK LFGAEHANVQ PHSGSQANMA VYFAVLNPGD TIMGMDLAHG 130 140 150 160 170 180 GHLTHGAKVN FSGKLYNVIH YGVNPETELI DYDQLYKLAK EHKPKLIVGG ASAYPRVFDW 190 200 210 220 230 240 AKMREIADEV GALFMVDMAH YAGLIAGGVY PNPVPYAQFV TSTTHKTLRG PRSGFILTTK 250 260 270 280 290 300 EYAKAVDKSV FPGIQGGPLM HVIAAKAVAF KEAMSEEFKE YAKQVVENAR VLAEELKKYG 310 320 330 340 350 360 FKIVTGGTDS HIVLVDLRNK NIIGKDAEKA LEKAGITVNK NAVPFDPLPP TKTSGIRIGT 370 380 390 400 410 420 AALTTRGMKE DEMRKIAGWI NEVLSNMDDE KTIQRVRQEV RELCETFPLY PELKRRIDEL RSGKATDL

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06734.1.
PIR	D70343.
RefSeq	NP_213336.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66776.
SMR	O66776. Positions 1-405.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193194.
GenomeReviews	Gene locus aq_479 in contig AE000657_GR.
KEGG	aae:aq_479.
NMPDR	fig|224324.1.peg.351.
PATRIC	20958520. VBIAquAeo85532_0395.
Phylogenomic databases
HOGENOM	HBG301263.
OMA	FETFPYR.
PhylomeDB	O66776.
ProtClustDB	PRK00011.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_479-MONOMER.
Family and domain databases
HAMAP	MF_00051. SHMT. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00600.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_AQUAE

Accession

Primary (citable) accession number: O66776

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program