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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-343-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:aq_461
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001487571 – 478Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST478

Proteomic databases

PRIDEiO66766

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

STRINGi224324.aq_461

Structurei

Secondary structure

1478
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 16Combined sources12
Beta strandi23 – 26Combined sources4
Turni41 – 45Combined sources5
Helixi55 – 67Combined sources13
Beta strandi74 – 76Combined sources3
Beta strandi78 – 82Combined sources5
Beta strandi91 – 99Combined sources9
Beta strandi101 – 110Combined sources10
Beta strandi112 – 114Combined sources3
Beta strandi116 – 128Combined sources13
Beta strandi132 – 136Combined sources5
Beta strandi139 – 143Combined sources5
Beta strandi150 – 156Combined sources7
Helixi163 – 180Combined sources18
Helixi187 – 189Combined sources3
Beta strandi191 – 202Combined sources12
Beta strandi212 – 215Combined sources4
Helixi220 – 239Combined sources20
Beta strandi248 – 252Combined sources5
Turni253 – 256Combined sources4
Beta strandi257 – 259Combined sources3
Helixi285 – 293Combined sources9
Helixi299 – 309Combined sources11
Helixi314 – 322Combined sources9
Helixi324 – 336Combined sources13
Helixi340 – 349Combined sources10
Helixi351 – 358Combined sources8
Helixi362 – 364Combined sources3
Helixi369 – 380Combined sources12
Helixi386 – 399Combined sources14
Helixi403 – 410Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H0LX-ray2.30B/E/H/K/N/Q/T/W1-478[»]
3H0MX-ray2.80B/E/H/K/N/Q/T/W1-478[»]
3H0RX-ray3.00B/E/H/K/N/Q/T/W1-478[»]
ProteinModelPortaliO66766
SMRiO66766
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66766

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CHT Bacteria
COG0064 LUCA
HOGENOMiHOG000223742
InParanoidiO66766
KOiK02434
OMAiSDGNMQE
OrthoDBiPOG091H01A7

Family and domain databases

Gene3Di1.10.10.410, 1 hit
HAMAPiMF_00121 GatB, 1 hit
InterProiView protein in InterPro
IPR004413 Apn/Gln-ADT_bsu
IPR017959 Asn/Gln-tRNA_amidoTrfase_suB/E
IPR006075 Asn/Gln-tRNA_Trfase_suB/E_cat
IPR018027 Asn/Gln_amidotransferase
IPR003789 Asn/Gln_tRNA_amidoTrase-B-like
IPR023168 GatB_Yqey_C
IPR017958 Gln-tRNA_amidoTrfase_suB_CS
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11659 PTHR11659, 1 hit
PfamiView protein in Pfam
PF02934 GatB_N, 1 hit
PF02637 GatB_Yqey, 1 hit
SMARTiView protein in SMART
SM00845 GatB_Yqey, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
SSF89095 SSF89095, 1 hit
TIGRFAMsiTIGR00133 gatB, 1 hit
PROSITEiView protein in PROSITE
PS01234 GATB, 1 hit

Sequencei

Sequence statusi: Complete.

O66766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEKYEAVIG LEIHVQMDTK TKMFCGCKVE FGAEPNTNVC PVCLGMPGAL
60 70 80 90 100
PIVNKRAVEY AIRASLALNC EVHEESVFAR KHYFYPDLPK GYQISQYEKP
110 120 130 140 150
LATNGWVELN LPNGEKKKVR IRRLHIEEDA GKNIHEGDKT LVDLNRAGTP
160 170 180 190 200
LMEIVTEPDI RTPEEARLFL EKLRNIMRYA GVSKADMEKG QLRCDINVSI
210 220 230 240 250
RPKGSKEFGT RVEIKNVNSF RFVQKALEYE IERQINVVEE GGEVVQETRT
260 270 280 290 300
FDPQTGKTYP MRTKEEAEDY RYFPDPDLVP LKVKKEWIEE IKKNMPELPD
310 320 330 340 350
QRFERLIKEY GLSEYEAGIL VNHKEVGDFF EEAVRHFKEP KGIVNWLIND
360 370 380 390 400
LLGLLRDKGI SIEESPVKPE HLAELVKLIK EKVISTKIGK EVIKEMVETG
410 420 430 440 450
KTPSQIVEEK GLKQITDENQ IKELVKKIFE KHPKEVERLK QGEEKLIGFF
460 470
VGQVMRETRG KANPQVVNKV IRELVKEV
Length:478
Mass (Da):55,041
Last modified:August 1, 1998 - v1
Checksum:iF725AE78944BD79A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06727.1
PIRiB70342
RefSeqiNP_213326.1, NC_000918.1
WP_010880264.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC06727; AAC06727; aq_461
GeneIDi1193092
KEGGiaae:aq_461
PATRICifig|224324.8.peg.382

Similar proteinsi

Entry informationi

Entry nameiGATB_AQUAE
AccessioniPrimary (citable) accession number: O66766
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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