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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-346-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:aq_461
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000148757Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

STRINGi224324.aq_461.

Structurei

Secondary structure

1
478
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1612Combined sources
Beta strandi23 – 264Combined sources
Turni41 – 455Combined sources
Helixi55 – 6713Combined sources
Beta strandi74 – 763Combined sources
Beta strandi78 – 825Combined sources
Beta strandi91 – 999Combined sources
Beta strandi101 – 11010Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi116 – 12813Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi139 – 1435Combined sources
Beta strandi150 – 1567Combined sources
Helixi163 – 18018Combined sources
Helixi187 – 1893Combined sources
Beta strandi191 – 20212Combined sources
Beta strandi212 – 2154Combined sources
Helixi220 – 23920Combined sources
Beta strandi248 – 2525Combined sources
Turni253 – 2564Combined sources
Beta strandi257 – 2593Combined sources
Helixi285 – 2939Combined sources
Helixi299 – 30911Combined sources
Helixi314 – 3229Combined sources
Helixi324 – 33613Combined sources
Helixi340 – 34910Combined sources
Helixi351 – 3588Combined sources
Helixi362 – 3643Combined sources
Helixi369 – 38012Combined sources
Helixi386 – 39914Combined sources
Helixi403 – 4108Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0LX-ray2.30B/E/H/K/N/Q/T/W1-478[»]
3H0MX-ray2.80B/E/H/K/N/Q/T/W1-478[»]
3H0RX-ray3.00B/E/H/K/N/Q/T/W1-478[»]
ProteinModelPortaliO66766.
SMRiO66766. Positions 4-402.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66766.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
InParanoidiO66766.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEKYEAVIG LEIHVQMDTK TKMFCGCKVE FGAEPNTNVC PVCLGMPGAL
60 70 80 90 100
PIVNKRAVEY AIRASLALNC EVHEESVFAR KHYFYPDLPK GYQISQYEKP
110 120 130 140 150
LATNGWVELN LPNGEKKKVR IRRLHIEEDA GKNIHEGDKT LVDLNRAGTP
160 170 180 190 200
LMEIVTEPDI RTPEEARLFL EKLRNIMRYA GVSKADMEKG QLRCDINVSI
210 220 230 240 250
RPKGSKEFGT RVEIKNVNSF RFVQKALEYE IERQINVVEE GGEVVQETRT
260 270 280 290 300
FDPQTGKTYP MRTKEEAEDY RYFPDPDLVP LKVKKEWIEE IKKNMPELPD
310 320 330 340 350
QRFERLIKEY GLSEYEAGIL VNHKEVGDFF EEAVRHFKEP KGIVNWLIND
360 370 380 390 400
LLGLLRDKGI SIEESPVKPE HLAELVKLIK EKVISTKIGK EVIKEMVETG
410 420 430 440 450
KTPSQIVEEK GLKQITDENQ IKELVKKIFE KHPKEVERLK QGEEKLIGFF
460 470
VGQVMRETRG KANPQVVNKV IRELVKEV
Length:478
Mass (Da):55,041
Last modified:August 1, 1998 - v1
Checksum:iF725AE78944BD79A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06727.1.
PIRiB70342.
RefSeqiNP_213326.1. NC_000918.1.
WP_010880264.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06727; AAC06727; aq_461.
GeneIDi1193092.
KEGGiaae:aq_461.
PATRICi20958494. VBIAquAeo85532_0382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06727.1.
PIRiB70342.
RefSeqiNP_213326.1. NC_000918.1.
WP_010880264.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0LX-ray2.30B/E/H/K/N/Q/T/W1-478[»]
3H0MX-ray2.80B/E/H/K/N/Q/T/W1-478[»]
3H0RX-ray3.00B/E/H/K/N/Q/T/W1-478[»]
ProteinModelPortaliO66766.
SMRiO66766. Positions 4-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06727; AAC06727; aq_461.
GeneIDi1193092.
KEGGiaae:aq_461.
PATRICi20958494. VBIAquAeo85532_0382.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
InParanoidiO66766.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-346-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO66766.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiGATB_AQUAE
AccessioniPrimary (citable) accession number: O66766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.