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O66746 (GLPK_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycerol kinase
EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:aq_434
OrganismAquifex aeolicus (strain VF5)
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186

Sequence similarities

Belongs to the FGGY kinase family.

Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glycerol kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Glycerol kinase HAMAP MF_00186
PRO_0000059430

Regions

Nucleotide binding403 – 4075ATP By similarity

Sites

Binding site111Substrate By similarity
Binding site151ATP By similarity
Binding site791Substrate By similarity
Binding site1291Substrate By similarity
Binding site2381Substrate By similarity
Binding site2601ATP By similarity
Binding site3021ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
O66746 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: CE95F0E8FF593B37

FASTA49255,298
        10         20         30         40         50         60 
MSVVISIDVG TTRVKVIAFS KNGKIVAISD REVSQIYPEP GWVEQDPLEL WEAVRKSLSE 

        70         80         90        100        110        120 
VIQQVGLKEI NSIGITNQRE TVILWDKETG RPVYNAILWQ DLRTEDICRK LSEYSEYIKE 

       130        140        150        160        170        180 
NTGLLLHPYF SASKVNWIIE NVNGVKKDIE RGKVIFGTVD TWILWNLTGG KVHKTEPSNA 

       190        200        210        220        230        240 
SRTLLFNIKR LEYDDELLKI FRIPKNILPE VNESSSLFGY TDKSITGVEI PITGILGDQQ 

       250        260        270        280        290        300 
ASLFAHGIRE LEEVKNTYGT GLFLMLLTGS KPIIPERLLG TVAWVINGKV NYAIEGSVLT 

       310        320        330        340        350        360 
GGACIKWLMD RLKLIKQAAD TEYLAKSISS NEGVYFVPAF SGLGAPYWDA SARGIIIGIT 

       370        380        390        400        410        420 
GRTRIEHIAR AALEAIAYQT RDVIEEMEKE TGVKIKILKA DGGASQNNFL MQFQADILGI 

       430        440        450        460        470        480 
PVERPRHVEL TALGAAGIAG IYSGMWKSKE EFIEDTREVE LTFSPEMKKE ERELYYSKWK 

       490 
DAVKRAMKWS ML

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC06710.1.
PIRF70339.
RefSeqNP_213306.1. NC_000918.1.

3D structure databases

ProteinModelPortalO66746.
SMRO66746. Positions 2-491.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1193072.
GenomeReviewsGene locus aq_434 in contig AE000657_GR.
KEGGaae:aq_434.
NMPDRfig|224324.1.peg.321.
PATRIC20958446. VBIAquAeo85532_0359.

Phylogenomic databases

HOGENOMHBG511469.
OMAQATESET.
PhylomeDBO66746.

Enzyme and pathway databases

BioCycAAEO224324:AQ_434-MONOMER.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
[Tree]
InterProIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
KOK00864.
PANTHERPTHR10196. FGGY_kin. 1 hit.
PTHR10196:SF9. Glycerol_kin. 1 hit.
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. Glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_AQUAE
AccessionPrimary (citable) accession number: O66746
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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