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Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.UniRule annotation

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).UniRule annotation
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylaseUniRule annotation (EC:3.1.1.96UniRule annotation)
Short name:
DTDUniRule annotation
Alternative name(s):
D-tyrosyl-tRNA(Tyr) deacylaseCurated
Gly-tRNA(Ala) deacylaseUniRule annotation (EC:3.1.1.-UniRule annotation)
Gene namesi
Name:dtdUniRule annotation
Ordered Locus Names:aq_428
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001645131 – 148D-aminoacyl-tRNA deacylaseAdd BLAST148

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_428.

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 15Combined sources14
Beta strandi18 – 32Combined sources15
Helixi39 – 51Combined sources13
Turni66 – 70Combined sources5
Beta strandi72 – 77Combined sources6
Helixi79 – 82Combined sources4
Beta strandi86 – 90Combined sources5
Helixi99 – 114Combined sources16
Beta strandi120 – 122Combined sources3
Beta strandi125 – 127Combined sources3
Beta strandi130 – 144Combined sources15
Helixi145 – 147Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DBOX-ray2.76A1-148[»]
ProteinModelPortaliO66742.
SMRiO66742.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66742.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 138Gly-cisPro motif, important for rejection of L-amino acidsUniRule annotation2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.UniRule annotation

Sequence similaritiesi

Belongs to the DTD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYA. Bacteria.
COG1490. LUCA.
HOGENOMiHOG000113981.
InParanoidiO66742.
KOiK07560.
OMAiDGPVTIW.
OrthoDBiPOG091H02D0.

Family and domain databases

CDDicd00563. Dtyr_deacylase. 1 hit.
Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiView protein in InterPro
IPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom.
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiView protein in Pfam
PF02580. Tyr_Deacylase. 1 hit.
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequencei

Sequence statusi: Complete.

O66742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVIQRVKK SWVEVDGKVV GSINEGLNVF LGVRKGDTEE DIEKLVNKIL
60 70 80 90 100
NLRIFEDERG KFQYSVLDIK GEILVVSQFT LYANVKKGRR PSFEEAEEPK
110 120 130 140
RAKELYEKFV DKIKESGLKV ETGIFGAMMD VFIENWGPVT IIIDSREI
Length:148
Mass (Da):16,968
Last modified:August 1, 1998 - v1
Checksum:i24A3324C96C21234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06704.1.
PIRiB70339.
RefSeqiNP_213302.1. NC_000918.1.
WP_010880240.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06704; AAC06704; aq_428.
GeneIDi1193068.
KEGGiaae:aq_428.
PATRICifig|224324.8.peg.353.

Similar proteinsi

Entry informationi

Entry nameiDTD_AQUAE
AccessioniPrimary (citable) accession number: O66742
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: August 1, 1998
Last modified: September 27, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families