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Protein

Threonine synthase

Gene

thrC2

Organism
Aquifex aeolicus (strain VF5)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.UniRule annotation

Catalytic activityi

O-phospho-L-homoserine + H2O = L-threonine + phosphate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-threonine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-threonine from L-aspartate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (thrA)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC2), Threonine synthase (thrC1)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891Pyridoxal phosphateUniRule annotation
Binding sitei319 – 3191Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesisUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-319-MONOMER.
UniPathwayiUPA00050; UER00065.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthaseUniRule annotation (EC:4.2.3.1UniRule annotation)
Gene namesi
Name:thrC2Imported
Ordered Locus Names:aq_425Imported
OrganismiAquifex aeolicus (strain VF5)Imported
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei63 – 631N6-(pyridoxal phosphate)lysineUniRule annotationCombined sources

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_425.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZSJX-ray1.80A/B/C/D1-352[»]
ProteinModelPortaliO66740.
SMRiO66740. Positions 4-331.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66740.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 320297PALPInterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1935Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the threonine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5I. Bacteria.
COG0498. LUCA.
HOGENOMiHOG000076503.
InParanoidiO66740.
KOiK01733.
OMAiSGGWFDE.
OrthoDBiEOG6HMX9M.

Family and domain databases

InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR004450. Thr_synthase-like.
IPR026260. Thr_Synthase_bac/arc.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF038945. Thr_synthase. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRWQGIIKQ YKKYLPVDEN TPIVTLYEGN TPLIEADNLA RAIGFKGKIY
60 70 80 90 100
LKYEGLNPTG SFKDRGMTLA ISKAVEAGKR AVICASTGNT SASAAAYAAR
110 120 130 140 150
AGLRAYVLLP KGAVAIGKLS QAMIYGAKVL AIQGTFDDAL NIVRKIGENF
160 170 180 190 200
PVEIVNSVNP YRIEGQKTAA FEICDTLGEA PDYHFIPVGN AGNITAYWKG
210 220 230 240 250
FKIYYEEGKI TKLPRMMGWQ AEGAAPIVKG YPIKNPQTIA TAIKIGNPYS
260 270 280 290 300
WKSALKAAQE SGGKIDAVSD SEILYAYKLI ASTEGVFCEP ASAASVAGLI
310 320 330 340 350
KLVREGFFKG GEVVTCTLTG NGLKDPDTAI KVCEEPITVP PDFDEVVKVL

GF
Length:352
Mass (Da):37,809
Last modified:August 1, 1998 - v1
Checksum:i3BF480E6E097ACD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06690.1.
PIRiH70338.
RefSeqiNP_213300.1. NC_000918.1.
WP_010880238.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06690; AAC06690; aq_425.
GeneIDi1193066.
KEGGiaae:aq_425.
PATRICi20958430. VBIAquAeo85532_0351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06690.1.
PIRiH70338.
RefSeqiNP_213300.1. NC_000918.1.
WP_010880238.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZSJX-ray1.80A/B/C/D1-352[»]
ProteinModelPortaliO66740.
SMRiO66740. Positions 4-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06690; AAC06690; aq_425.
GeneIDi1193066.
KEGGiaae:aq_425.
PATRICi20958430. VBIAquAeo85532_0351.

Phylogenomic databases

eggNOGiENOG4107R5I. Bacteria.
COG0498. LUCA.
HOGENOMiHOG000076503.
InParanoidiO66740.
KOiK01733.
OMAiSGGWFDE.
OrthoDBiEOG6HMX9M.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00065.
BioCyciAAEO224324:GJBH-319-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO66740.

Family and domain databases

InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR004450. Thr_synthase-like.
IPR026260. Thr_Synthase_bac/arc.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF038945. Thr_synthase. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5Imported.
  2. "Crystal structure of threonine synthase from Aquifex aeolicus VF5."
    Mizutani H., Kunishima N.
    Submitted (SEP-2008) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS), PYRIDOXAL PHOSPHATE AT LYS-63.

Entry informationi

Entry nameiO66740_AQUAE
AccessioniPrimary (citable) accession number: O66740
Entry historyi
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.