O66728 (O66728_AQUAE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Poly A polymerase EMBL AAC06692.1

Gene names

Name:	pcnB1 EMBL AAC06692.1
Ordered Locus Names:	aq_411

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate. SAAS SAAS002646
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. RuleBase RU003953

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS002646 Nucleotide-binding RNA-binding RuleBase RU003953 SAAS SAAS002646
Molecular function	Nucleotidyltransferase SAAS SAAS002646 Transferase
Technical term	3D-structure PDB 1VFG Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O66728 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: A5B627695C2DAF73	FASTA	824	94,658
	10 20 30 40 50 60 MVCPKVVILS EGADLDSLSA AYGVLKLYPD AYLLKPKHLS KKAGEVFKKY RDKFRVIEDL 70 80 90 100 110 120 PDCFELVLVD THFLPEGLPR ERIKRIIVYD HHPIGDVKEF EGKIEKVGAA TTLVVEEIKE 130 140 150 160 170 180 KGIDINPRDA TLLAFGIYED TGNFTYEGTT PRDALALAFL LEKGANLREI REVVMETYTP 190 200 210 220 230 240 EQIEAVGKIV QSIEKVFING RQISFATAVL ERYQPDINTL LYEIKDLKES DAFFVIIEAE 250 260 270 280 290 300 GKTYVFGRSQ SEDVDVGEIL SHFGGGGHRE AGAVKLENVS AERIKELIKA FLKRKYVKLK 310 320 330 340 350 360 VRDIMNTPPF VLEEHVSVKD ALTELSERGI ANAPVINREG KLVGIISKKA LLKLVKLYPD 370 380 390 400 410 420 EPIELFVNRD FYTLSPDAPV WEAEEILTKF GQKLIPVVED GTVVGVVTRL DILQAVKEDL 430 440 450 460 470 480 EKLKEKRRKI KVPENIEEIA REVGQIAKEM GLRAYIVGGV VRDILLGKEV WDVDFVVEGN 490 500 510 520 530 540 AIELAKELAR RHGVNVHPFP EFGTAHLKIG KLKLEFATAR RETYPRPGAY PKVEPASLKE 550 560 570 580 590 600 DLIRRDFTIN AMAISVNLED YGTLIDYFGG LRDLKDKVIR VLHPVSFIED PVRILRALRF 610 620 630 640 650 660 AGRLNFKLSR STEKLLKQAV NLGLLKEAPR GRLINEIKLA LREDRFLEIL ELYRKYRVLE 670 680 690 700 710 720 EIIEGFQWNE KVLQKLYALR KVVDWHALEF SEERIDYGWL YLLILISNLD YERGKHFLEE 730 740 750 760 770 780 MSAPSWVRET YKFMKFKLGS LKEELKKAKE NYEVYRLLKP LHTSVLLLLM LEEELKEKIK 790 800 810 820 LYLEKLRKVK LPKEKIEELK KQGLKGKELG ERIEELKREI MNKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
[2]	"Structural basis for template-independent RNA polymerization." Tomita K., Fukai S., Ishitani R., Ueda T., Takeuchi N., Vassylyev D.G., Nureki O. Nature 430:700-704(2004) [PubMed: 15295603] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 443-824.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC06692.1.

PIR

D70337.

RefSeq

NP_213288.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VFG	X-ray	2.80	A/B	443-824	[»]

ProteinModelPortal

O66728.

SMR

O66728. Positions 443-824.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1193054.

GenomeReviews

Gene locus aq_411 in contig AE000657_GR.

KEGG

aae:aq_411.

NMPDR

fig|224324.1.peg.303.

PATRIC

20958404. VBIAquAeo85532_0338.

Phylogenomic databases

HOGENOM

HBG616788.

OMA

PLNGHDL.

PhylomeDB

O66728.

ProtClustDB

CLSK2748308.

Family and domain databases

InterPro

IPR000644. Cysta_beta_synth_core.
IPR003156. Pesterase_DHHA1.
IPR001667. Pesterase_RecJ.
IPR002646. PolA_pol_head_dom.
[Graphical view]

KO

K00970.

Pfam

PF00571. CBS. 2 hits.
PF01368. DHH. 1 hit.
PF02272. DHHA1. 1 hit.
PF01743. PolyA_pol. 1 hit.
[Graphical view]

SMART

SM00116. CBS. 2 hits.
[Graphical view]

PROSITE

PS51371. CBS. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O66728_AQUAE

Accession

Primary (citable) accession number: O66728

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)