Reviewed, UniProtKB/Swiss-Prot O66727 (CARA_AQUAE)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	aq_410

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112245
Regions
	Domain	189 – 371	183	Glutamine amidotransferase type-1
	Region	1 – 185	185	CPSase HAMAP MF_01209
Sites
	Active site	264	1	Nucleophile By similarity
	Active site	348	1	By similarity
	Active site	350	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66727-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 3EF695880690F582	FASTA	371	41,683
	10 20 30 40 50 60 MRKTAILALE DGSYFVGYSF GAEGETGGEL VFNTSMTGYQ EILTDPSYKG QIVVMTYTQI 70 80 90 100 110 120 GNYGVNDEDI ESKSVQVNGF VVKEAFFRYS NWRAKKSLDE YLKENNVVGI YGIDTRALVK 130 140 150 160 170 180 KIREKGSMKG VISTVEKDPK KLVQKARELP DISEQNLVEE VSAKDIYYWN QGDYDPRRGF 190 200 210 220 230 240 LYRENEKPLV AVIDFGVKFN ILRRLVSEGA KVVVVPPENA KEAIEKINPD AIFLSNGPGD 250 260 270 280 290 300 PERVISGIRL TREYMEKKPI MGICLGCQII GLALGGKTYK LKFGHHGGNH PVKDLRTGKI 310 320 330 340 350 360 EITAQNHNFA IDPESLPEDV EVTHLNLLDN TVEGIKHKHL PIFAVQYHPE NSPGPHDSYY 370 LFKEFVKMAQ G

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC06674.1.
PIR	C70337.
RefSeq	NP_213287.1.
3D structure databases
HSSP	HSSP built from PDB template 1CS0 based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1193053.
GenomeReviews	Gene locus aq_410 in contig AE000657_GR.
KEGG	aae:aq_410.
NMPDR	fig|224324.1.peg.302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O66727.
OMA	O66727. LFDGSNC.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_410-MON.
BRENDA	6.3.5.5. 189781.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_AQUAE

Accession

Primary (citable) accession number: O66727

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)