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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, C-terminal section (carB2), Carbamoyl-phosphate synthase large chain, N-terminal section (carB1), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BRENDAi2.1.3.2. 396.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:aq_409
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001130871 – 291Aspartate carbamoyltransferaseAdd BLAST291

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_409.

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi12 – 26Combined sources15
Beta strandi36 – 43Combined sources8
Helixi47 – 58Combined sources12
Beta strandi62 – 67Combined sources6
Helixi68 – 70Combined sources3
Helixi72 – 75Combined sources4
Helixi79 – 88Combined sources10
Beta strandi92 – 100Combined sources9
Helixi106 – 110Combined sources5
Beta strandi112 – 121Combined sources10
Turni122 – 124Combined sources3
Helixi127 – 141Combined sources15
Beta strandi148 – 153Combined sources6
Helixi159 – 170Combined sources12
Beta strandi174 – 179Combined sources6
Helixi181 – 183Combined sources3
Helixi188 – 191Combined sources4
Beta strandi193 – 198Combined sources6
Helixi199 – 205Combined sources7
Beta strandi207 – 211Combined sources5
Helixi216 – 218Combined sources3
Beta strandi223 – 225Combined sources3
Helixi227 – 234Combined sources8
Helixi238 – 241Combined sources4
Turni255 – 257Combined sources3
Helixi261 – 263Combined sources3
Beta strandi264 – 266Combined sources3
Helixi271 – 289Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D6NX-ray2.30B1-291[»]
4BJHX-ray2.20B1-291[»]
ProteinModelPortaliO66726.
SMRiO66726.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66726.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiO66726.
KOiK00609.
OMAiGPINREI.
OrthoDBiPOG091H00PQ.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLISSLDL TREEVEEILK YAKEFKEGKE ETIKASAVLF FSEPSTRTRL
60 70 80 90 100
SFEKAARELG IETYLVSGSE SSTVKGESFF DTLKTFEGLG FDYVVFRVPF
110 120 130 140 150
VFFPYKEIVK SLNLRLVNAG DGTHQHPSQG LIDFFTIKEH FGEVKDLRVL
160 170 180 190 200
YVGDIKHSRV FRSGAPLLNM FGAKIGVCGP KTLIPRDVEV FKVDVFDDVD
210 220 230 240 250
KGIDWADVVI WLRLQKERQK ENYIPSESSY FKQFGLTKER FEKVKLYMHP
260 270 280 290
GPVNRNVDID HELVYTEKSL IQEQVKNGIP VRKAIYKFLW T
Length:291
Mass (Da):33,558
Last modified:August 1, 1998 - v1
Checksum:i93523D3DBDD01534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06675.1.
PIRiB70337.
RefSeqiNP_213286.1. NC_000918.1.
WP_010880224.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06675; AAC06675; aq_409.
GeneIDi1193052.
KEGGiaae:aq_409.
PATRICi20958400. VBIAquAeo85532_0336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06675.1.
PIRiB70337.
RefSeqiNP_213286.1. NC_000918.1.
WP_010880224.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D6NX-ray2.30B1-291[»]
4BJHX-ray2.20B1-291[»]
ProteinModelPortaliO66726.
SMRiO66726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06675; AAC06675; aq_409.
GeneIDi1193052.
KEGGiaae:aq_409.
PATRICi20958400. VBIAquAeo85532_0336.

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiO66726.
KOiK00609.
OMAiGPINREI.
OrthoDBiPOG091H00PQ.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BRENDAi2.1.3.2. 396.

Miscellaneous databases

EvolutionaryTraceiO66726.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRB_AQUAE
AccessioniPrimary (citable) accession number: O66726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.