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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, C-terminal section (carB2), Carbamoyl-phosphate synthase large chain, N-terminal section (carB1), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-305-MONOMER.
BRENDAi2.1.3.2. 396.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:aq_409
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Aspartate carbamoyltransferasePRO_0000113087Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_409.

Structurei

Secondary structure

1
291
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Helixi12 – 2615Combined sources
Beta strandi36 – 438Combined sources
Helixi47 – 5812Combined sources
Beta strandi62 – 676Combined sources
Helixi68 – 703Combined sources
Helixi72 – 754Combined sources
Helixi79 – 8810Combined sources
Beta strandi92 – 1009Combined sources
Helixi106 – 1105Combined sources
Beta strandi112 – 12110Combined sources
Turni122 – 1243Combined sources
Helixi127 – 14115Combined sources
Beta strandi148 – 1536Combined sources
Helixi159 – 17012Combined sources
Beta strandi174 – 1796Combined sources
Helixi181 – 1833Combined sources
Helixi188 – 1914Combined sources
Beta strandi193 – 1986Combined sources
Helixi199 – 2057Combined sources
Beta strandi207 – 2115Combined sources
Helixi216 – 2183Combined sources
Beta strandi223 – 2253Combined sources
Helixi227 – 2348Combined sources
Helixi238 – 2414Combined sources
Turni255 – 2573Combined sources
Helixi261 – 2633Combined sources
Beta strandi264 – 2663Combined sources
Helixi271 – 28919Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D6NX-ray2.30B1-291[»]
4BJHX-ray2.20B1-291[»]
ProteinModelPortaliO66726.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO66726.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiO66726.
KOiK00609.
OMAiWCDVANM.
OrthoDBiEOG61KBJZ.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O66726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLISSLDL TREEVEEILK YAKEFKEGKE ETIKASAVLF FSEPSTRTRL
60 70 80 90 100
SFEKAARELG IETYLVSGSE SSTVKGESFF DTLKTFEGLG FDYVVFRVPF
110 120 130 140 150
VFFPYKEIVK SLNLRLVNAG DGTHQHPSQG LIDFFTIKEH FGEVKDLRVL
160 170 180 190 200
YVGDIKHSRV FRSGAPLLNM FGAKIGVCGP KTLIPRDVEV FKVDVFDDVD
210 220 230 240 250
KGIDWADVVI WLRLQKERQK ENYIPSESSY FKQFGLTKER FEKVKLYMHP
260 270 280 290
GPVNRNVDID HELVYTEKSL IQEQVKNGIP VRKAIYKFLW T
Length:291
Mass (Da):33,558
Last modified:August 1, 1998 - v1
Checksum:i93523D3DBDD01534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06675.1.
PIRiB70337.
RefSeqiNP_213286.1. NC_000918.1.
WP_010880224.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC06675; AAC06675; aq_409.
GeneIDi1193052.
KEGGiaae:aq_409.
PATRICi20958400. VBIAquAeo85532_0336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC06675.1.
PIRiB70337.
RefSeqiNP_213286.1. NC_000918.1.
WP_010880224.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D6NX-ray2.30B1-291[»]
4BJHX-ray2.20B1-291[»]
ProteinModelPortaliO66726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06675; AAC06675; aq_409.
GeneIDi1193052.
KEGGiaae:aq_409.
PATRICi20958400. VBIAquAeo85532_0336.

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiO66726.
KOiK00609.
OMAiWCDVANM.
OrthoDBiEOG61KBJZ.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BioCyciAAEO224324:GJBH-305-MONOMER.
BRENDAi2.1.3.2. 396.

Miscellaneous databases

EvolutionaryTraceiO66726.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPYRB_AQUAE
AccessioniPrimary (citable) accession number: O66726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.