O66726 (PYRB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	aq_409

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 291

291

Aspartate carbamoyltransferase HAMAP MF_00001

PRO_0000113087

Secondary structure

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291

Helix Strand Turn

Details...

Helix

7 – 9

3

Helix

12 – 26

15

Beta strand

36 – 43

8

Helix

46 – 58

13

Beta strand

62 – 66

5

Helix

79 – 86

8

Beta strand

93 – 97

5

Helix

106 – 110

5

Beta strand

113 – 118

6

Helix

132 – 140

9

Beta strand

148 – 152

5

Helix

159 – 162

4

Helix

166 – 170

5

Helix

181 – 183

3

Helix

188 – 191

4

Beta strand

197 – 199

3

Beta strand

207 – 211

5

Helix

217 – 220

4

Beta strand

223 – 225

3

Helix

227 – 233

7

Helix

238 – 241

4

Turn

255 – 257

3

Helix

261 – 263

3

Beta strand

264 – 266

3

Helix

271 – 276

6

Helix

280 – 289

10

Sequences

	Sequence		Length	Mass (Da)
	O66726 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 93523D3DBDD01534	FASTA	291	33,558
	10 20 30 40 50 60 MRSLISSLDL TREEVEEILK YAKEFKEGKE ETIKASAVLF FSEPSTRTRL SFEKAARELG 70 80 90 100 110 120 IETYLVSGSE SSTVKGESFF DTLKTFEGLG FDYVVFRVPF VFFPYKEIVK SLNLRLVNAG 130 140 150 160 170 180 DGTHQHPSQG LIDFFTIKEH FGEVKDLRVL YVGDIKHSRV FRSGAPLLNM FGAKIGVCGP 190 200 210 220 230 240 KTLIPRDVEV FKVDVFDDVD KGIDWADVVI WLRLQKERQK ENYIPSESSY FKQFGLTKER 250 260 270 280 290 FEKVKLYMHP GPVNRNVDID HELVYTEKSL IQEQVKNGIP VRKAIYKFLW T

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC06675.1.

PIR

B70337.

RefSeq

NP_213286.1. NC_000918.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3D6N	X-ray	2.30	B	1-291	[»]

ProteinModelPortal

O66726.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1193052.

GenomeReviews

Gene locus aq_409 in contig AE000657_GR.

KEGG

aae:aq_409.

NMPDR

fig|224324.1.peg.301.

PATRIC

20958400. VBIAquAeo85532_0336.

Phylogenomic databases

HOGENOM

HBG579429.

OMA

LQRERMS.

PhylomeDB

O66726.

ProtClustDB

CLSK2299385.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_409-MONOMER.

Family and domain databases

HAMAP

MF_00001. Asp_carb_tr.
[Tree]

InterPro

IPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002082. Asp_carbamoyltransf_euk.
[Graphical view]

KO

K00609.

Pfam

PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]

PRINTS

PR00100. AOTCASE.
PR00101. ATCASE.

SUPFAM

SSF53671. Asp/Orn_carbamoyltranf. 1 hit.

TIGRFAMs

TIGR00670. Asp_carb_tr. 1 hit.

PROSITE

PS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PYRB_AQUAE

Accession

Primary (citable) accession number: O66726

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program