O66686 (TPIS_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Synonyms:	timA
Ordered Locus Names:	aq_360

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Triosephosphate isomerase HAMAP MF_00147_B		PRO_0000090173
Sites
	Active site	94	1	Electrophile By similarity
	Active site	165	1	Proton acceptor By similarity
	Binding site	8	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O66686 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B89DD88F1EF5C068	FASTA	247	27,765
	10 20 30 40 50 60 MRRLIAANWK MNKTVKETEE YINTFLKFVE HPESREILIC PPFTSLYVAG KMLQGTGVKL 70 80 90 100 110 120 GAQNCHYEKR GAFTGEISIP MLQEVGCEYV IVGHSERRHI FGESDELIHK KIVACLEMGI 130 140 150 160 170 180 RPILCVGEKK EEREAGMTFK VIETQIKLAL TGVEEHTDKI DIAYEPVWAI GTGTPATPED 190 200 210 220 230 240 AVEVHTFIRN LINQLNPKNE GKTRILYGGS VNPQNAKEFM KHEEINGLLV GTASLDPESF AKIVYSF

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC06639.1.
PIR	B70332.
RefSeq	NP_213246.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O66686.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193012.
GenomeReviews	Gene locus aq_360 in contig AE000657_GR.
KEGG	aae:aq_360.
NMPDR	fig|224324.1.peg.261.
PATRIC	20958308. VBIAquAeo85532_0290.
Phylogenomic databases
HOGENOM	HBG708281.
OMA	ANTVIAY.
PhylomeDB	O66686.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_360-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_AQUAE

Accession

Primary (citable) accession number: O66686

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program